The phototoxicity of photofrin was enhanced by PEGylated liposome in vitro

Sadzuka, Yasuyuki; Tokutomi, Koji; Iwasaki, Fumiaki; Sugiyama, Ikumi; Hirano, Tôru; Konno, Hiroyuki; Oku, Naoto; Sonobe, Takashi

doi:10.1016/j.canlet.2005.10.024

Cited by 40 publications

(20 citation statements)

References 21 publications

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“…4,5 Photosensitizers, light, and oxygen are three basic components. Although many photosensitizers have been reported for their potential of application in PDT, such as photofrin, hematoporphyrin derivatives, 5-aminolevulinic acid (ALA) and silicon phthalocyanine (Pc4), [6][7][8] so far there are only a few photosensitizers under clinical applications. It might be the results of hydrophobia and poor photophysical and physicochemical properties of these photosensitizers.…”

Section: Introductionmentioning

confidence: 99%

Spectroscopic analysis of the interaction between tetra-(p-sulfoazophenyl-4-aminosulfonyl)-substituted aluminum (III) phthalocyanines and serum albumins

Zheng

Lin

et al. 2017

J. Innov. Opt. Health Sci.

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The binding interaction between tetra-(p-sulfoazophenyl-4-aminosulfonyl)-substituted aluminum (III) phthalocyanine (AlPc), and two-serum albumins (bovine serum albumin (BSA) and human serum albumin (HSA)) has been investigated. AlPc could quench the intrinsic°uorescence of BSA and HSA through a static quenching process. The primary and secondary binding sites of AlPc on BSA were domain I and III of BSA. The primary binding site of AlPc on HSA was domain I, and the secondary binding sites of AlPc on HSA were found at domains I and II. Our results suggest that AlPc readily interact with BSA and HSA implying that the amphiphilic substituents AlPc may contribute to their transportation in the blood.

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Section: Introductionmentioning

confidence: 99%

Spectroscopic analysis of the interaction between tetra-(p-sulfoazophenyl-4-aminosulfonyl)-substituted aluminum (III) phthalocyanines and serum albumins

Zheng

Lin

et al. 2017

J. Innov. Opt. Health Sci.

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“…It is obvious that liposomalization may enhance phototoxicity, especially by PEGmodification, and this was significantly higher than that of photofrin in solution or photofrin in liposomes. However, photofrin-PEG-liposomes inhibited the uptake of photofrin into tumor cells [36]. In our studies, we also assessed the effectiveness of ethosomal formulation of 5-ALA, as the previous results showed that the average particle sizes of the ethosomes were less than those of liposomes, and indicated that the penetration ability of ethosomes was greater than that of liposomes [22].…”

Section: Discussionmentioning

confidence: 97%

“…Egg yolk phosphatidyl choline (PC), phosphatidic acid (PA) and phosphatidyl glycerol (PG) were employed in the preparation of these liposomes. In our study, in preparing precursors for in vitro and in vivo studies, we used similar substrates as other authors did with photofrin [36]. It is obvious that liposomalization may enhance phototoxicity, especially by PEGmodification, and this was significantly higher than that of photofrin in solution or photofrin in liposomes.…”

Section: Discussionmentioning

confidence: 99%

In vitro and in vivo matrix metalloproteinase expression after photodynamic therapy with a liposomal formulation of aminolevulinic acid and its methyl ester

Osiecka

Jurczyszyn

Symonowicz

et al. 2010

Cellular and Molecular Biology Letters

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colony stimulating factor; HNSCC -head and neck squamous cell carcinoma; IL-1 -interleukin-1; met-ALA -methyl ester of aminolevulinic acid; MMPs -matrix metalloproteinases; m-THPC -meso-tetra(hydroxyphenyl)chlorine; PDT -photodynamic therapy; PPIX -protoporphyrin IX; ROS -reactive oxygen species, TIMPs -tissue inhibitors of matrix metalloproteinases; VEGF -vascular endothelial growth factor Abstract: Photodynamic therapy (PDT) is a well-known method for the treatment of malignant tumors, and its principles have been well established over the past 30 years. This therapy involves the application of a chemical called a photosensitizer and its subsequent excitation with light at the appropriate wavelength and energy. Topical photodynamic therapy with aminolevulinic acid (5-ALA) is an alternative therapy for many malignant processes, including nonmelanoma skin cancers such as basal-cell carcinoma (BCC). Our novel approach for this study was to use a liposomal formulation of 5-ALA and its methyl ester (commercially available as metvix) both in vitro and in vivo, and to check whether the liposome-entrapped precursors of photosensitizers can induce the expression of metalloproteinases (MMPs) in animal tumor cells and in other tissues from tumor-bearing rats and in selected cell lines in vitro. We also checked whether the application of tissue inhibitors of matrix metalloproteinases Unauthenticated Download Date | 5/11/18 3:12 AM CELLULAR & MOLECULAR BIOLOGY LETTERS 631(TIMPs) has any effect on MMPs in the above-mentioned experimental models, and if they can cause complete inhibition of MMP expression. Immunohistochemical studies revealed that after the PDT, the intensity of expression of MMPs in healthy animals was very low and seen in single cells only. After the PDT in tumor-bearing rats, MMP-3 was expressed in the tumor cells with the highest intensity of staining in the tissues directly adjacent to the tumors, while MMP-2 and -9 were not found. In the control groups, there was no observed expression of MMPs. In vitro studies showed that MMP-3 was expressed in MCF-7 cells after PDT, but MMP-9 was not observed and MMP-2 was only seen in single cases. Our studies confirmed that the application of an MMP-3 inhibitor may block an induction of MMP-3 expression which had previously been initiated by PDT. The preliminary data obtained from cancer patients revealed that new precursors are effective in terms of PDT, and that using MMP inhibitors should be considered as a potential enhancing factor in clinical PDT.

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“…As demonstrated in this article, it resulted in PDT treatment with T DL of r10 min. Liposomal formulation also enhanced the phototoxicity of Photofrin [Sadzuka et al, 2006]. Previously, we had shown that human amelanotic melanomas implanted in nude mice disintegrated when subjected to PDT with i.v.…”

Section: Advantage Of Hydrophobic Photosensitizersmentioning

confidence: 94%

“…The LDL pathway may be facilitated by liposomal formulations of photosensitizers [Polo et al, 2002]. Various types of delivery systems have been proposed as carriers for improving the selectivity of antitumor drugs [Konan et al, 2002], especially porphyrin-based photosensitizers, including monoclonal antibodies [Rahimipour et al, 2003], liposomes [Cuomo et al, 1990;Love et al, 1996;Ježek et al, 2003;Konan et al, 2002;Derycke et al, 2004;Kepczynski et al, 2006;Sadzuka et al, 2006], microspheres and nanoparticles [Yan and Kopelman, 2003;Roy et al, 2003;Ricci and Marchetti, 2006], growth factors, hormones, and LDLs [Polo et al, 2002].…”

mentioning

confidence: 99%

Optimum modality for photodynamic therapy of tumors: gels containing liposomes with hydrophobic photosensitizers

Nekvasil

Zadinová

Tahotná

et al. 2007

Drug Development Research

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Photodynamic therapy (PDT) has entered an era of clinical acceptance; however, for its wider use, including drug formulations for topical applications, it must have a rapid effect and be free of skinphotosensitization effects. Preclinical studies of two prototype formulations exhibiting successful therapeutic rates based on translucent gels containing liposomes with hydrophobic photosensitizers are presented. Experimental PDT of human tumors implanted in nude mice with liposomal gels containing meso-tetrakisphenylporphyrin (TPP) or hydroxy-aluminum phthalocyanine (PC) caused significant dystrophy of human basal cell carcinoma, amelanotic melanoma, breast carcinoma, and colorectal carcinoma. Whereas the drug-to-light time interval for TPP was 12 h, much less than that of most commercially available approved photosensitizers, 10 min was sufficient to disintegrate the effects of the PC liposomal gel. Liposomes containing hydrophobic photosensitizers were prepared either by extrusion through 100-nm filters or using a microfluidizer. Histology of tumors in remission showed existence of overall necrosis and the presence of only small islets of tumorous cells in incomplete remission. Fibrinoid necrosis of the tumors was a visible sign of the therapeutic effect. TPP and PC from the gel were distributed by circulation to the organs and tissues in a different and more favorable pattern than by intravenous injection. We conclude that hydrophobic photosensitizers are preferable and more efficient for PDT (Ježek et al., Int J Cancer 103:693-702, 2003) and their liposomal gel formulations are predicted to be optimum preparations enabling wide ranging use of topical PDT for numerous cancer indications, initially for nonmelanoma skin cancer. Drug Dev Res 68: [235][236][237][238][239][240][241][242][243][244][245][246][247][248][249][250][251][252] 2007 r 2007 Wiley-Liss, Inc.

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The phototoxicity of photofrin was enhanced by PEGylated liposome in vitro

Cited by 40 publications

References 21 publications

Spectroscopic analysis of the interaction between tetra-(p-sulfoazophenyl-4-aminosulfonyl)-substituted aluminum (III) phthalocyanines and serum albumins

Spectroscopic analysis of the interaction between tetra-(p-sulfoazophenyl-4-aminosulfonyl)-substituted aluminum (III) phthalocyanines and serum albumins

In vitro and in vivo matrix metalloproteinase expression after photodynamic therapy with a liposomal formulation of aminolevulinic acid and its methyl ester

Optimum modality for photodynamic therapy of tumors: gels containing liposomes with hydrophobic photosensitizers

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