The Photobiomodulation of Vital Parameters of the Cancer Cell Culture by Low Dose of Near-IR Laser Irradiation

Abstract

The mechanisms underlining the cell adaptive and/or activating oxidative stress called low level light (LLLT) or photobiomodulation therapies (PBMT) still remain unclear for near-infrared (NIR) spectrum range (750-3000 nm) especially for 1265-1270 nm range (highest absorption by molecular oxygen). It is most probably that the mitochondria may also appear to be the main target for these wavelengths. It is known that mitochondria can generate ROS under visible and 800-1060 nm spectrum range irradiation which in turn control voltage-dependent anion channels (VDAC). Here we investigated cellular damage caused by low doses of 1265-70 nm laser radiation regarding to VDAC activity, the level of oxidative stress, malondialdehyde (MDA) content, cell viability, mitochondrial potential and mass, GSH level, mitochondrial and nuclear DNA damage in the cancer cell culture exposed to low-level laser irradiation at 1265 nm. We used continuous wave laser with output power 4 mW, the energy 

High-throughput single-cell live imaging of photobiomodulation with multispectral near-infrared lasers in cultured T cells

“…15,19 Interestingly, recent studies demonstrated that a brief exposure of the skin with NIR laser ranging from 1061 to 1301 nm at an irradiance of 0.5 to 5 W∕cm 2 augmented the immune response to intradermal vaccination and conferred protection. [20][21][22][23][24][25] In these studies, the activation of innate responses, subsequent migration of skinresident dendritic cells, and augmentation of adaptive response were dependent on the generation of ROS. 21 It has also been reported that broadband NIR light (760 to 1440 nm) induces the formation of mitochondria-derived ROS and subsequent increase in redox potential in cultured human dermal fibroblasts.…”

Воздействие Лазерного Излучения С Длиной Волны 1265 Нм На Культуру Клеток Аденокарциномы Человека

“…Параметры лазерного излучения. В качестве источника низкоинтенсивного лазерного излучения мы использовали полупроводниковый узкополосный (высококогерентный) лазер с шириной линии излучения ~ 0,001 нм (Yenista Optics, OSICS T100 Tunable Laser Module T100 1310) [14].…”

“…Nowadays laser therapy is widely used in dentistry [7,8], photodynamic therapy [9,10], skindisease and cosmetic defects treatment [11,12] with the use of photosensitizers and without their use. It is known that the various range of wavelength can cause diametrically opposite cellular effects from damage [13,14] up to regeneration [15,16], which have great perspectives both for treatment and rehabilitation after it.…”

“…На сегодняшний день лазерная терапия широко используется в стоматологии [7,8], фотодинамической терапии [9,10], лечении кожных заболеваний и косметических недостатков [11,12] с использованием фотосенсибилизаторов и без него. Известно, что различные диапазоны длин волн могут вызывать диаметрально противоположные клеточные эффекты -от повреждения [13,14] до регенерации [15,16], что имеет большие перспективы как для лечения, так и для реабилитации после него.…”

Anti-inflammatory and cell proliferative effect of the 1270 nm laser irradiation on the BALB/c nude mouse model involves activation of the cell antioxidant system

“…The strongest resonance absorption band of oxygen is in the range of 1265-1270 nm, where no absorption of known chromophores occurs in the living cell. The light-oxygen effect (1265-1270 nm ranges) can activate or damage (depending on the energy density) the biosystems through a direct photoexcitation of molecular triplet oxygen into the singlet oxygen [4][5][6][7][8][9].…”