The phosphorylation and characterisation of soybean isolate and its ?-conglycinin component by casein kinase II

Burghoffer, Chantal; Chardot, Thierry; Meunier, Jean‐Claude

doi:10.1002/(sici)1097-0010(19990701)79:9<1179::aid-jsfa345>3.0.co;2-q

Cited by 1 publication

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References 20 publications

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“…Phosphorylation of the proteins would lower the pI, with the extent of the decrease dependent on the number of phosphates added. Whether seed LOXs are phosphorylated in vivo is not known to us; however, Burghoffer et al (1999) have reported the in vitro phosphorylation of soybean seed LOX using casein kinase. Analysis of pea LOX sequences using algorithms of Blom et al (1999), available online (www.cbs.dtu.dk/services/NetPhos/), suggests the existence of multiple putative phosphorylation sites, lending credence to this possibility.…”

Section: Discussionmentioning

confidence: 99%

Changes in lipoxygenase isoforms during germination and early seedling growth of Pisum sativum L.

Koster

2006

Seed Sci. Res.

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Investigations of physiological functions of seed lipoxygenase during germination and early seedling growth are complicated by the presence of multiple isoforms with different product specificity. The combination of isoelectric focusing, lipoxygenase in-gel activity staining and mass spectrometry showed that only two seed lipoxygenases, LOX-2 and LOX-3, were active at neutral pH in protein extracts from dry and germinating pea seeds (Pisum sativum L.), with LOX-3 more abundant than LOX-2. Each active seed LOX appeared at multiple pIs, probably resulting from post-translational modifications. The abundance of pea seed lipoxygenases was lower in embryonic shoots than in other parts of the axis. After radicle emergence, seed LOXs were degraded, as revealed by Western blotting using antibodies specific to pea seed LOXs. Two new vegetative isoforms, LOX-N3 and LOX-g, appeared in the shoot and gradually replaced the pea seed lipoxygenases as the principal contributors to LOX enzyme activity during early seedling growth. Activities of LOX-N3 and LOX-g were abundant in shoot tips, but not root tips, in pea seedlings. Thus, during germination, there is a shift of LOX activity from radicles to shoots that accompanies the transition from seed LOXs to vegetative LOXs. These results confirmed that identification of pea LOXs based solely on pI may be misleading; however, mass spectrometry enabled more accurate identification of these highly homologous proteins to be made.

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Section: Discussionmentioning

confidence: 99%

Changes in lipoxygenase isoforms during germination and early seedling growth of Pisum sativum L.

Koster

2006

Seed Sci. Res.

View full text Add to dashboard Cite

show abstract

The phosphorylation and characterisation of soybean isolate and its ?-conglycinin component by casein kinase II

Cited by 1 publication

References 20 publications

Changes in lipoxygenase isoforms during germination and early seedling growth of Pisum sativum L.

Changes in lipoxygenase isoforms during germination and early seedling growth of Pisum sativum L.

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