The phosphoinositide signature guides the final step of plant cytokinesis

Lebecq, Alexis; Goldy, Camila; Fangain, Aurélie; Gascon, Elsa; Belcram, Katia; Pastuglia, Martine; Bouchez, David; Caillaud, Marie‐Cécile

doi:10.1126/sciadv.adf7532

Cited by 7 publications

(3 citation statements)

References 57 publications

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“…During the late stages of phragmoplast expansion, actin is needed for the completion of cell plate fusion (van Oostende-Triplet et al ., 2017), potentially in part dependent on recruitment of TAN1 and other division site localized proteins. Recruitment of other division site proteins (e.g POK2) to de novo cell plate fusion sites have also been observed in mutants which generate additional ectopic cell plates, suggesting that de novo localization may be a common feature during cytokinesis (Lebecq et al ., 2023).…”

Section: Resultsmentioning

confidence: 99%

De NovoTANGLED1 Recruitment to Aberrant Cell Plate Fusion Sites in Maize

Uyehara,

Diep,

Allsman

et al. 2024

Preprint

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Division plane positioning is critical for proper growth and development in many organisms. In plants, the division plane is established before mitosis, by accumulation of a cytoskeletal structure called the preprophase band (PPB). The PPB is thought to be essential for recruitment of division site localized proteins, which remain at the division site after the PPB disassembles. Here, we show that a division site localized protein, TANGLED1 (TAN1), is recruited independently of the PPB to the cell cortex at sites, by the plant cytokinetic machinery, the phragmoplast. TAN1 recruitment to de novo sites on the cortex is partially dependent on intact actin filaments and the myosin XI motor protein OPAQUE1 (O1). These data imply a yet unknown role for TAN1 and possibly other division site localized proteins during the last stages of cell division when the phragmoplast touches the cell cortex to complete cytokinesis.

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Section: Resultsmentioning

confidence: 99%

De NovoTANGLED1 Recruitment to Aberrant Cell Plate Fusion Sites in Maize

Uyehara,

Diep,

Allsman

et al. 2024

Preprint

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show abstract

“…Recruitment of other division site proteins (e.g. POK1) to de novo cell plate fusion sites have also been observed in mutants that generate additional ectopic cell plates, suggesting that de novo localization might be a common feature during cytokinesis ( Lebecq et al, 2023 ).…”

Section: Resultsmentioning

confidence: 99%

De novo TANGLED1 recruitment from the phragmoplast to aberrant cell plate fusion sites in maize

Uyehara,

Diep,

Allsman

et al. 2024

Journal of Cell Science

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Division plane positioning is critical for proper growth and development in many organisms. In plants, the division plane is established before mitosis, by accumulation of a cytoskeletal structure called the preprophase band (PPB). The PPB is thought to be essential for recruitment of division site localized proteins, which remain at the division site after the PPB disassembles. Here, we show that a division site localized protein, TANGLED1 (TAN1), is recruited independently of the PPB to the cell cortex by the plant cytokinetic machinery, the phragmoplast, using the PPB-defective mutant discordia1 and chemical treatments that disrupt the phragmoplast. TAN1 recruitment to de novo sites on the cortex is partially dependent on intact actin filaments and the myosin XI motor protein OPAQUE1 (O1). These data imply a yet unknown role for TAN1 and possibly other division site localized proteins during the last stages of cell division when the phragmoplast touches the cell cortex to complete cytokinesis.

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“…Defective regulation of proteins controlling membrane trafficking/remodeling provides an alternative explanation for abnormal cell plate shapes in the α-Aurora mutant. Recently, loss of the phosphoinositide phosphatase SAC9 was found to cause aberrant enrichment of MAP65-3 at the inner edge of mature cell plates and formation of branched cell plates 46 . Given the similar branched cell plate phenotype in both mutants, exploring the interplay between α-Aurora signaling and phosphoinositide pathways presents an opportunity to delineate their coordinated functions in precisely controlling cell plate expansion and maturation.…”

Section: Discussionmentioning

confidence: 99%

Arabidopsis α-Aurora kinase plays a role in cytokinesis through regulating MAP65-3 association with microtubules at phragmoplast midzone

Deng,

Xiao,

Tang

et al. 2024

Nat Commun

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The α-Aurora kinase is a crucial regulator of spindle microtubule organization during mitosis in plants. Here, we report a post-mitotic role for α-Aurora in reorganizing the phragmoplast microtubule array. In Arabidopsis thaliana, α-Aurora relocated from spindle poles to the phragmoplast midzone, where it interacted with the microtubule cross-linker MAP65-3. In a hypomorphic α-Aurora mutant, MAP65-3 was detected on spindle microtubules, followed by a diffuse association pattern across the phragmoplast midzone. Simultaneously, phragmoplast microtubules remained belatedly in a solid disk array before transitioning to a ring shape. Microtubules at the leading edge of the matured phragmoplast were often disengaged, accompanied by conspicuous retentions of MAP65-3 at the phragmoplast interior edge. Specifically, α-Aurora phosphorylated two residues towards the C-terminus of MAP65-3. Mutation of these residues to alanines resulted in an increased association of MAP65-3 with microtubules within the phragmoplast. Consequently, the expansion of the phragmoplast was notably slower compared to wild-type cells or cells expressing a phospho-mimetic variant of MAP65-3. Moreover, mimicking phosphorylation reinstated disrupted MAP65-3 behaviors in plants with compromised α-Aurora function. Overall, our findings reveal a mechanism in which α-Aurora facilitates cytokinesis progression through phosphorylation-dependent restriction of MAP65-3 associating with microtubules at the phragmoplast midzone.

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The phosphoinositide signature guides the final step of plant cytokinesis

Cited by 7 publications

References 57 publications

De NovoTANGLED1 Recruitment to Aberrant Cell Plate Fusion Sites in Maize

De NovoTANGLED1 Recruitment to Aberrant Cell Plate Fusion Sites in Maize

De novo TANGLED1 recruitment from the phragmoplast to aberrant cell plate fusion sites in maize

Arabidopsis α-Aurora kinase plays a role in cytokinesis through regulating MAP65-3 association with microtubules at phragmoplast midzone

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