The phosphoesterase of pea plants (<i>Pisum sativum</i> L.)

Pierpoint, W. S.

doi:10.1042/bj0670466

Cited by 9 publications

(1 citation statement)

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“…The function of a-glycerophosphate is connected with lipid synthesis. The curves of the activity of glncose-6-phosphatase and a-glycerophosphatase in different phases of the growth curve of sycamore cells and their pH optima (about 6) are so similar that it is apparent that there are only a few unspecific phosphafases in this plant (Figures 4, 5 and 9 A), as in several other species studied (Pierpoint 1957, Ikawa et al 1964, Shaw 1966. Therefore it is not sure which are the most important real substrates in the cells.…”

Section: Resultssupporting

confidence: 59%

Changes in the Activity of Certain Enzymes of Acer pseudoplatanus L. Cells at four Stages of Growth in Suspension Culture

Simola

Sopanen

1970

Physiologia Plantarum

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The activities of twelve enzymes were studied in Acer psendoplatanum L. cells grown in suspension cultures for 6, 12, 19 and 22 days. The enzyme activities of cells (6 and 12 days) in the phase of rapid cell division were rather different from that of cells in the stationary phase (19, 22 days). The activity of aldolasc was highest at the first two stages of growth, when the amount of RNA and protein per cell was highest, and after this decreased steeply. The curves of specific activities of several phosphatases (glucosc‐6‐phosphatasc, α‐glycerophosphatase, acid phosphatase and ATPase) and ribonuclease were almost the reverse of the curve of aldolase, showing a minimum in 12‐day‐old cells and a maximum in cells 19 and 22 days old. The activity of peroxidase was also high in ageing cells. Glutamate: oxaloacetate transaminase and aminopeptidases (leucine and alanine) had synchronous maxima (6 and 19 days) of specific activity but the activity of aminopeptidases decreased gradually during ageing of the suspension, if the enzyme activities are calculated per 107 cells. Glutamate: pyruvate transaminasc activity was very low and no dcoxyribonuclease activity could be detected.

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Section: Resultssupporting

confidence: 59%