The phosphatase reaction as an aid to the identification of micro‐organisms using phenolphthalein phosphate as substrate

Abstract

REACTIONS depending on bacterial phosphatases have not hitherto been used for the identification of micro-organisms, principally because of difKculties in technique. Gordon and Cooper ( 1932) established the presence of glycerophosphatase in Bact. coli and staphylococci. Boivin and Mesrobeanu (1933) showed that Bact. wli and Staph. awe= have two phosphatase systems active a t pH 7 and 10. Heard and Wynne (1933) and Pett and Wynne (1933), using ground up organisms and glycerophosphate, hexosediphosphate and pyrophosphate as substrates, studied the phospbatase systems of CZ. acetobutyliczcm and Propioniboct, jensenii, and Pett and Wynne (1938) of Aerobacter aerogenes, Alkaligenes fmcalis and B. subtilis. Leahy, Sandholzer and Woodside (1939) demonstrated phosphatase in the " lyophilised " cells of various Cramnegative bacilli (Serratia, Pseudomonas, Escherichia, Aerobacter, Chromobacter, Proteus, Salmonella, Shigella, Eberthella and Bact. alkaligenes) using disodium phenyl phosphate as a substrate. Leahy, Stokinger and Carpenter (1940) showed that phosphatase was present in Neisseria. In all these investigations the cells employed were either non-viable or not multiplying.

Síntese e avaliação de azoderivado de 2-fenilbenzoxazol como substrato fluorogênico na diferenciação de Candida spp.

“…A partir de 1940 ensaios biológicos começaram a ser explorados para a diferenciação de microorganismos com base na metabolização de corantes. Os primeiros trabalhos foram realizados em cultura de células bacterianas crescendo sobre meio sólido, ao qual era incorporada a fenolftaleína (BRAY;KING, 1943).…”

Bacteriology of Erysipeloid

“…A trace of acid but no clot in litmus milk, Methyl red, Voges-Proskauer, and tests for indole and ammonia, for nitrate and methylene-blue reduction, for hydrolysis of sodium hippurate, and for urease and catalase were negative. A moderate degree of phosphatase activity was detected (Bray and King, 1943). No growth in Koser's citrate.…”

Detection of bacterial phosphatase activity by means of an original and simple test.

“…Phosphatase production has also been studied in many other microorganisms, including streptococci (Taketo and Taketo, 1974), corynebacteria (Bray and King, 1943), enterobacteria (Bayliss et al, 1948;Wolf et al, 1972;Bhatti and Done, 1974), anaerobic bacteria (Porschen and Spaulding, 1974), and yeasts (Bayliss et al, 1948;Smith et al, 1973).…”