The PhoP/Q two-component regulatory system is essential for Xylella fastidiosa survival in Vitis vinifera grapevines

Pierce, Brittany; Kirkpatrick, Bruce C.

doi:10.1016/j.pmpp.2014.12.003

Cited by 9 publications

(3 citation statements)

References 36 publications

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“…No PD symptom was observed in the grapevines inoculated with popP deletion strain in compared with severe PD symptom developed by grapevines infected with wild type X. fastidiosa and complementary strain XfΔpopP-C consistently observed in repeated greenhouse inoculation tests. Similarity results were recently reported on the pathogenicity of Xf popP [37]. Our results demonstrate that popP is an important virulence factors and plays critical role for the survivals and the pathogenicity of X. fastidiosa.…”

Section: Discussionsupporting

confidence: 91%

Characterization of Xylella fastidiosa popP Gene Required for Pathogenicitya

Lin¹

2015

J Plant Pathol Microbiol

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Xylella fastidiosa (Xf) possess a two component regulatory system (TCS) PopP-PopQ which differentially regulates genes in response to environmental stimuli. To elucidate the role of popP in the pathogenicity of X. fastidiosa causing Pierce's disease (PD) of grapevine, a site-directed deletion method and chromosome-based genetic complementation strategy were employed to create popP deletion mutant XfΔpopP and its complementary strain XfΔpopP-C. In vitro studies showed that while all strains had similar growth curves, XfΔpopP showed significant reduction in cell-cell aggregation and cell-matrix adherence. Biofilm production of XfΔpopP was about 42% less than that of wild type X. fastidiosa and XfΔpopP-C. No symptoms were observed in grapevines inoculated with XfΔpopP, whereas grapevines inoculated with wild type X. fastidiosa and XfΔpopP-C showed typical PD symptoms. Several biofilm-related genes and genes involving protein secretary systems were down regulated in XfΔpopP in compared with wild type X. fastidiosa and XfΔpopP-C. These in vitro and in planta assay results provide strong evidence that the role of PopP is required for pathogenicity of X. fastidiosa on grapevine.

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Section: Discussionsupporting

confidence: 91%

Characterization of Xylella fastidiosa popP Gene Required for Pathogenicitya

Lin¹

2015

J Plant Pathol Microbiol

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“…Interestingly, genes involved in production (rpfF) and sensing (rpfC and rpfG) of the quorumsensing molecule diffusible signal factor (DSF), which is critical for acquisition of X. fastidiosa by insects from plants [62], and regulates plant host specificity [69], were detected in high frequencies. Other regulatory genes detected here, have been shown to be important for virulence of X. fastidiosa; interestingly, phoP/phoQ [70] were shown to be needed for survival in plants.…”

Section: Recombinant Regions Shared Among Subspecies Inform Eco-evolumentioning

confidence: 67%

Patterns of inter- and intrasubspecific homologous recombination inform eco-evolutionary dynamics of Xylella fastidiosa

et al. 2019

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High rates of homologous recombination (HR) in the bacterial plant pathogen Xylella fastidiosa have been previously detected. This study aimed to determine the extent and explore the ecological significance of HR in the genomes of recombinants experimentally generated by natural transformation and wild-type isolates. Both sets of strains displayed widespread HR and similar average size of recombined fragments consisting of random events (2-10 kb) of inter-and intrasubspecific recombination. A significantly higher proportion and greater lengths (>10 kb, maximum 31.5 kb) of recombined fragments were observed in subsp. morus and in strains isolated in Europe from intercepted coffee plants shipped from the Americas. Such highly recombinant strains pose a serious risk of emergence of novel variants, as genetically distinct and formerly geographically isolated genotypes are brought in close proximity by global trade. Recently recombined regions in wild-type strains included genes involved in regulation and signaling, host colonization, nutrient acquisition, and host evasion, all fundamental traits for X. fastidiosa ecology. Identification of four recombinant loci shared between wild-type and experimentally generated recombinants suggests potential hotspots of recombination in this naturally competent pathogen. These findings provide insights into evolutionary forces possibly affecting the adaptive potential to colonize the host environments of X. fastidiosa.

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“…PhoQ, a transmembrane histidine kinase, is autophosphorylated upon sensing physiological signals and subsequently phosphorylates the corresponding response regulator PhoP [40][41][42][43][44]. The PhoP/PhoQ TCS is important for Xylella fastidiosa survival in planta as well as biofilm formation and cell-cell aggregation [45]. PmrB is another sensor kinase responding to environmental stimuli, and PmrA is the corresponding response regulator.…”

Section: Introductionmentioning

confidence: 99%

Remodeling of Lipid A in Pseudomonas syringae pv. phaseolicola In Vitro

Gerster

Wröbel

Hofstaedter

et al. 2022

IJMS

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Pseudomonas species infect a variety of organisms, including mammals and plants. Mammalian pathogens of the Pseudomonas family modify their lipid A during host entry to evade immune responses and to create an effective barrier against different environments, for example by removal of primary acyl chains, addition of phosphoethanolamine (P-EtN) to primary phosphates, and hydroxylation of secondary acyl chains. For Pseudomonas syringae pv. phaseolicola (Pph) 1448A, an economically important pathogen of beans, we observed similar lipid A modifications by mass spectrometric analysis. Therefore, we investigated predicted proteomes of various plant-associated Pseudomonas spp. for putative lipid A-modifying proteins using the well-studied mammalian pathogen Pseudomonas aeruginosa as a reference. We generated isogenic mutant strains of candidate genes and analyzed their lipid A. We show that the function of PagL, LpxO, and EptA is generally conserved in Pph 1448A. PagL-mediated de-acylation occurs at the distal glucosamine, whereas LpxO hydroxylates the secondary acyl chain on the distal glucosamine. The addition of P-EtN catalyzed by EptA occurs at both phosphates of lipid A. Our study characterizes lipid A modifications in vitro and provides a useful set of mutant strains relevant for further functional studies on lipid A modifications in Pph 1448A.

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The PhoP/Q two-component regulatory system is essential for Xylella fastidiosa survival in Vitis vinifera grapevines

Cited by 9 publications

References 36 publications

Characterization of Xylella fastidiosa popP Gene Required for Pathogenicitya

Characterization of Xylella fastidiosa popP Gene Required for Pathogenicitya

Patterns of inter- and intrasubspecific homologous recombination inform eco-evolutionary dynamics of Xylella fastidiosa

Remodeling of Lipid A in Pseudomonas syringae pv. phaseolicola In Vitro

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