The phenotypic and functional characteristics of umbilical cord blood and peripheral blood natural killer cells

Verneris, Michael R.; Miller, Jeffrey S.

doi:10.1111/j.1365-2141.2009.07768.x

Cited by 86 publications

(72 citation statements)

References 52 publications

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“…by guest www.bloodjournal.org From UCBT (eg, patient 1, Figure 2A). Notably, CD56 Ϫ CD16 ϩ NK cells were poorly represented in adult controls (2% Ϯ 2%; n ϭ 60), whereas, as previously described, they were consistently detectable in CB ( Figure 2B), 29,30 but their percentage was considerably lower than in patients 1, 4, 5, and 6 (Figure 2B-C).…”

Section: Maturation Of Nk-cell Subsets During Nk-cell Reconstitutionsupporting

confidence: 72%

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Phenotypic and functional heterogeneity of human NK cells developing after umbilical cord blood transplantation: a role for human cytomegalovirus?

Chiesa

Falco

Podestà

et al. 2012

Blood

231

190

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Natural killer (NK) cells play a crucial role in early immunity after hematopoietic stem cell transplantation because they are the first lymphocyte subset recovering after the allograft. In this study, we analyzed the development of NK cells after intrabone umbilical cord blood (CB) transplantation in 18 adult patients with hematologic malignancies. Our data indicate that, also in this transplantation setting, NK cells are the first lymphoid population detectable in peripheral blood.

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Section: Maturation Of Nk-cell Subsets During Nk-cell Reconstitutionsupporting

confidence: 72%

“…As shown in Figure 6, both these subsets had a limited functional capability, although rhIL-15 could increase degranulation in agreement with previous studies on CB-NK cell-mediated cytotoxicity. 29,30 …”

Section: Org Frommentioning

confidence: 99%

Phenotypic and functional heterogeneity of human NK cells developing after umbilical cord blood transplantation: a role for human cytomegalovirus?

Chiesa

Falco

Podestà

et al. 2012

Blood

231

190

View full text Add to dashboard Cite

show abstract

“…In the present study CB the NK (CD16 + /CD56 + ) cell ratio was similar to the MPB NK cell ratio, as was previously reported for NK cells in APB and CB [36]. The nature of CB NK cells remains controversial; despite the similar frequency of NK cells in CB and APB, CB NK cell dysfunction, as compared to APB and MPB was reported.…”

Section: Akdeniz Et Al Differences In Cord Bloodsupporting

confidence: 47%

Differences in lymphocyte subpopulation count and function in cord, maternal and adult blood

Akdeniz¹,

Aktaş²,

Erten³

et al. 2011

Turk J Hematol

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Objective: Phenotypical characterization and functional activity of lymphocytes and natural killer (NK) cells in cord blood (CB) were investigated, and maternal peripheral blood (MPB) values were compared to those of adult peripheral blood (APB) (control). Materials and Methods: To determine cytotoxic activity target cells (K562) were labeled with carboxyfluorescein diacetate (CFDA) or fluorescein isothiocyanate (FITC), and propidium iodide (PI) was used to label dead cells. Cell surface expression in CB, APB, and MPB cells were analyzed using flow cytometry. Results: CB and MPB mononuclear cells had similar CD45, CD34, CD4, and surface molecule for T helper cell expression, but had low-level expression of total T-lymphocyte surface molecules CD3 and CD8. CD19 and HLA-DR expression was higher in CB than in MPB. The same high-level of expression for CD19 and HLA-DR was observed in APB, as compared to MPB. All other cell surface expressions were similar in APB and MPB samples. NK (CD16 + and CD56 + ) cells in CB was similar to that in MPB and APB, and the level of inhibitory KIR receptors in NK cells was higher in venous CB than in MPB and APB. The only difference between MPB and APB was that the CD158a level was higher in MPB. No difference was observed in NK cells in CB and MPB, in terms of cytotoxicity. Conclusion: The present results show that there was numerical and proportional variability of lymphocytes and their subgroups in CB and APB, but no cytological difference. (Turk J Hematol 2011; 28: 33-41) Key words: NK activity, cord blood lymphocytes, flow cytometry

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“…26 Because MuHV-4 infection in mice shares important properties with Epstein-Barr virus infection in humans, we speculate that this mode of NK-cell arming may occur in healthy people. Of note, CD56 dim NK cells with cytotoxic capacity have been identified in umbilical cord blood, 27 suggesting that multiple pathways may lead to functionally competent human NK cells. Thus, some of the differences between human and SPF laboratory mouse NK-cell function may reflect a disparity in immune activation induced by chronic virus infection, as opposed to an intrinsic difference in the NK cells themselves.…”

Section: Discussionmentioning

confidence: 99%

Latent herpesvirus infection arms NK cells

White

Keppel²,

Schneider³

et al. 2010

Blood

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The phenotypic and functional characteristics of umbilical cord blood and peripheral blood natural killer cells

Cited by 86 publications

References 52 publications

Phenotypic and functional heterogeneity of human NK cells developing after umbilical cord blood transplantation: a role for human cytomegalovirus?

Phenotypic and functional heterogeneity of human NK cells developing after umbilical cord blood transplantation: a role for human cytomegalovirus?

Differences in lymphocyte subpopulation count and function in cord, maternal and adult blood

Latent herpesvirus infection arms NK cells

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