The Phenotype and Function of Monocytes in Patients With Pulmonary Tuberculosis

Сахно, Л. В.; Тихонова, М. А.; Kozhevnikov, V. S.; Сенюков, В. В.; Пронкина, Н. В.; Никонов, С. Д.; Жданов, О. А.; Останин, А. А.; Черных, Е. Р.

doi:10.15789/1563-0625-2005-1-49-56

Cited by 2 publications

(2 citation statements)

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“…Nevertheless, we found an inverse correlation ( r S = −0.62, P < 0.01) between the number of CD14 + CD16 + cells and a percentage of monocytes with an intracellular TNF- α expression. Earlier we showed that the number of IL-10 + cells within CD16 + population is significantly higher in TB patients than in healthy subjects [ 15 ]. These data together with our present results showing that the most pronounced increase of CD14 + CD16 + cells in PPD-anergic patients was associated with the highest increase of IL-10 can testify to a high monocyte suppressive activity and its crucial role in suppressing an antigen specific response.…”

Section: Discussionmentioning

confidence: 99%

“…Importantly, blood monocytes represent an important source of APCs capable of migrating to the infected site and differentiating into macrophages and DCs. Despite the absence of direct infection of circulating monocytes with Mtb many studies reported an altered phenotype and functions of monocytes in pulmonary tuberculosis [ 15 – 17 ]. Given an important role of monocytes as precursors of DCs and macrophages, their dysfunctions can result in pronounced impairments of monocyte-derived DCs (MDDC) and monocyte-derived macrophages (MDM).…”

Section: Introductionmentioning

confidence: 99%

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Impairments of Antigen-Presenting Cells in Pulmonary Tuberculosis

Сахно¹,

Shevela²,

Тихонова³

et al. 2015

Journal of Immunology Research

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The phenotype and functional properties of antigen-presenting cells (APC), that is, circulating monocytes and generated in vitro macrophages and dendritic cells, were investigated in the patients with pulmonary tuberculosis (TB) differing in lymphocyte reactivity to M. tuberculosis antigens (PPD-reactive versus PPD-anergic patients). We revealed the distinct impairments in patient APC functions. For example, the monocyte dysfunctions were displayed by low CD86 and HLA-DR expression, 2-fold increase in CD14+CD16+ expression, the high numbers of IL-10-producing cells, and enhanced IL-10 and IL-6 production upon LPS-stimulation. The macrophages which were in vitro generated from peripheral blood monocytes under GM-CSF were characterized by Th1/Th2-balance shifting (downproduction of IFN-γ coupled with upproduction of IL-10) and by reducing of allostimulatory activity in mixed lymphocyte culture. The dendritic cells (generated in vitro from peripheral blood monocytes upon GM-CSF + IFN-α) were characterized by impaired maturation/activation, a lower level of IFN-γ production in conjunction with an enhanced capacity to produce IL-10 and IL-6, and a profound reduction of allostimulatory activity. The APC dysfunctions were found to be most prominent in PPD-anergic patients. The possible role of APC impairments in reducing the antigen-specific T-cell response to M. tuberculosis was discussed.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%