Separately living with parents causes adolescent girls who live in orphanages to have lower levels of positive affect. This study aimed to determine the effect of yoga training to increase positive affect adolescent girls in several orphanage in city of Makassar. The subject of the study (N = 10, age 15-19 years) is adolescent girls who lived in the orphanage in Makassar. This study used a quasi-experiment design with an experimental group and a control group. Both groups have a pre-test and post-test in 4th session, 8th session, and 12th session of yoga treatment. Three professional judgments validated the instrument of this study (Positive and Negative Affect Schedule-Expanded Form), with Aiken's V coefficient moves between 0.83 to 1.00. Scale's reliability measured by calculating the Cronbach alpha coefficient with a score of 0.81 for affective positive and 0.89 for negative. This study used data analysis techniques Friedman test with SPSS 22.0 for Windows. The results showed a positive Affect significance value of 0.002 (p <0.05), which means there is an effect of yoga training to increase positive affect of adolescent girls in the orphanage Makassar. Yoga can be an alternative training program that can be implemented in the orphanage to increase positive affect adolescent girls in the orphanage.