The pharmacology, pharmacokinetics, and toxicity of spinosin: A mini review

Kuang, Xiaolan; She, Ganshu; Ma, Tao; Cai, Wanna; Zhao, Jianmin; Liu, Bo; Xu, Fangfang

doi:10.3389/fphar.2022.938395

Cited by 3 publications

(1 citation statement)

References 55 publications

(56 reference statements)

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“…It was reported that total salvianolic acid improved brain function in rats by inhibiting apoptosis of CA1 neurons to improve global functional network topology [19]. Furthermore, spinosin has anxiolytic, sedative, and hypnotic effects [20]. The results indicated that these constituents may play important roles in the effects of JNNm.…”

Section: Discussionmentioning

confidence: 99%

Simultaneous determination of 19 constituents in the Jian‐Nao‐Ning mixture using high‐performance liquid chromatography‐tandem mass spectrometry

FU,

Ding,

Wang

et al. 2024

Separation Science Plus

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Jin‐Nao‐Ning mixture (JNNm) has been clinically used to treat insomnia in China. However, the major constituents of JNNm were not revealed. In this study, an ultra‐high‐performance liquid chromatography‐tandem mass spectrometry was established and validated for simultaneous quantitation of 19 representative constituents of JNNm. The method had good specificity and adequate sensitivity. The calibration curves of the constituents displayed good linearity (r > 0.990) in appropriate ranges. The intra‐ and inter‐day precision at three quality control (QC) levels ranged from 0.68% to 5.17% and 0.42% to 5.37%, respectively. The recovery ranged from 91.76% to 110.06%, with a precision of less than 5.07%. In addition, the method had good repeatability (relative standard deviation [RSD] < 4.93%). The analytes were all stable for 24 h (RSD < 7.98%) at room temperature. The results of the quantitative analysis showed that salvianolic acid B (495.7–606.3 μg/mL) is the most abundant constituent, followed by betaine (411.3–468.3 μg/mL), salvianic acid A (10.7–34.9 μg/mL), lobetyolin (11.6–14.6 μg/mL), spinosin (4.0–7.6 μg/mL), schisandrol A (1.5–4.1 μg/mL), and schisandrol B (1.2–1.5 μg/mL). Concentrations of other constituents were below 1 μg/mL. This study would be helpful for the QC of JNNm and the revelation of its effective constituents.

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Section: Discussionmentioning

confidence: 99%

Simultaneous determination of 19 constituents in the Jian‐Nao‐Ning mixture using high‐performance liquid chromatography‐tandem mass spectrometry

FU,

Ding,

Wang

et al. 2024

Separation Science Plus

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Spinosin inhibits activated hepatic stellate cell to attenuate liver fibrosis by targeting Nur77/ASK1/p38 MAPK signaling pathway

Lin,

Li,

Hong

et al. 2024

European Journal of Pharmacology

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Validation of the Ultra-Performance Liquid Chromatography with Tandem Mass Spectrometry Method for Simultaneous Analysis of Eighteen Compounds in the Traditional Herbal Prescription, Sanjoin-Tang

Seo

Shin

2023

Separations

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Sanjoin-tang (SJIT) is an ancient oriental medicine prescription listed in the Jinguiyaolue that is mainly used for the treatment of primary insomnia. This study was conducted to develop and validate an ultra-performance liquid chromatography with tandem mass spectrometry (UPLC–MS/MS) simultaneous analysis method for the quality control of SJIT using 18 target compounds. The 18 analytes were separated on an Acquity UPLC BEH C18 column maintained at 45 °C using a mobile phase composed of distilled water and acetonitrile. The MS system was used to simultaneously detect all analytes using the multiple reaction monitoring (MRM) method of Xevo TQ-XS coupled with an electrospray ionization source. The concentrations of the 18 analytes investigated in the SJIT samples ranged from below the limit of detection to 9.553 mg/g. In conclusion, the validated UPLC–MS/MS MRM analysis method can be used to obtain basic data to establish chemical-nonclinical linkage efficacy and for the clinical research and quality evaluation of SJIT.

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The pharmacology, pharmacokinetics, and toxicity of spinosin: A mini review

Cited by 3 publications

References 55 publications

Simultaneous determination of 19 constituents in the Jian‐Nao‐Ning mixture using high‐performance liquid chromatography‐tandem mass spectrometry

Simultaneous determination of 19 constituents in the Jian‐Nao‐Ning mixture using high‐performance liquid chromatography‐tandem mass spectrometry

Spinosin inhibits activated hepatic stellate cell to attenuate liver fibrosis by targeting Nur77/ASK1/p38 MAPK signaling pathway

Validation of the Ultra-Performance Liquid Chromatography with Tandem Mass Spectrometry Method for Simultaneous Analysis of Eighteen Compounds in the Traditional Herbal Prescription, Sanjoin-Tang

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