The pharmacological profile of ovalbumin‐induced paw oedema in rats

Feitosa, Regina Fátima Gonçalves; Melcíades, G B; Assreuy, Ana Maria Sampaio; Rocha, Marcos Fábio Gadelha; Ribeiro, Ronaldo A.; Lima, Aldo A. M.

doi:10.1080/09622935020138000

Cited by 24 publications

(20 citation statements)

References 30 publications

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“…In fact, there is substantial evidence that histamine and serotonin play important roles in the pathogenesis of allergic disorders [38,39] . For instance, histamine and serotonin signaling through H 1 and 5-HT 2 receptors, respectively, are known to be important mediators of edema formation during the immediate phase of allergic responses [40,41] . Interestingly, our data showed that although effectively precluding the increase in vascular permeability seen in the PCA, treatment with a combination of promethazine and methysergide, which block H 1 and 5-HT 2 receptors, respectively, was unable to modify the fluid consumption in OVA-immunized rats ( fig.…”

Section: Discussionmentioning

confidence: 99%

Pharmacological Manipulation of Immune-Induced Food Aversion in Rats

Zarzana

Basso²,

Costa-Pinto³

et al. 2008

Neuroimmunomodulation

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Background: Mice allergic to ovalbumin (OVA) avoid drinking a solution containing this antigen. This was interpreted as related to IgE-dependent mast cell degranulation and sensory C fiber activation. Methods: We employed pharmacological manipulation to further investigate the mediators involved in immune-induced food aversion. Results: While nonimmunized rats preferred a sweetened OVA solution, immunized rats avoided it. We also employed a paradigm in which rats are conditioned to drink water for two 10-min sessions a day. Tolerant rats presented lower IgE titers, and this manipulation abrogated food aversion. Dexamethasone (1.0 mg/kg) prevented the aversion of OVA-immunized rats to the antigen-containing solution. Combined blockade of H₁ and 5-hydroxytryptamine (5-HT)₂ receptors by promethazine (3.0 mg/kg) plus methysergide (5.0 mg/kg) was unable to alter food aversion. Blockade of 5-HT₃ receptors by ondansetron (1.0 mg/kg) caused a twofold increase in the ingestion of the sweetened OVA solution by immunized rats, suggesting the involvement of 5-HT₃ receptors in food aversion. Finally, we showed that dexamethasone or promethazine plus methysergide, but not ondansetron, effectively prevented the IgE-dependent mast-cell-degranulation-induced increase in vascular permeability in rats. Conclusion: We suggest that regardless of whether or not they cause edema, IgE-mediated mast cell degranulation and consequent 5-HT₃ signaling are involved in the process that triggers avoidance to the source of the allergen in allergic rats.

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Section: Discussionmentioning

confidence: 99%

Pharmacological Manipulation of Immune-Induced Food Aversion in Rats

Zarzana

Basso²,

Costa-Pinto³

et al. 2008

Neuroimmunomodulation

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“…; 1 h). 16,17) 2. Peritonitis Model: Pro-inflammatory Activity: C. feldmannii (0.9 mg/kg) or saline were injected intraperitoneally (1 ml; i.p.)…”

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confidence: 99%

Biological Effects of a Sulfated-Polysaccharide Isolated from the Marine Red Algae Champia feldmannii

Assreuy

Gomes

Silva

et al. 2008

Biological & Pharmaceutical Bulletin

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Sulfated-polysaccharides are complex macromolecular constituents of the extracellular matrix of marine algae that play an important role in mechanical, osmotic and ionic regulation.1) In Biomedicine the anticoagulant and antithrombotic properties have been most widely exploited and, at least for galactans, appear to be dependent on the sulfatation content and/or position of the sulfate groups.2) A natural sulfated polymer of L-fucose (fucoidan) showed a dose-dependent inhibition of leukocyte migration in the in vivo peritonitis model, 3) and sulfated fucans isolated from brown algae showed potent in vitro and in vivo inhibition of the human complement system. 4,5) It was demonstrated that a sulfatedpolysaccharide fraction extracted from the brown algae Porphyra haitanesis presents an in vivo antioxidant property, causing an increase in the spleen and thymus size, suggestive of an immunostimulant action. 6) Additionally, sulfated galactans of the red marine algae Bryothamnion seaforthii presented antinociceptive activity in mice 7) and of the red micro algae Pophyridium sp. anti-inflammatory action inhibiting eritema formation after topical use in humans.8) However, studies of sulfated galactans role in models of inflammation in vivo are rare in the literature. Here we isolated and investigated the effects of a sulfated galactan from the red marine algae Champia feldmannii, widely encountered along the South East and North East Brazilian sea coast, in experimental models of acute inflammation, coagulation and nociception. MATERIALS AND METHODS AnimalsMale Wistar rats (150-250 g) and Swiss mice (25-35 g) were maintained with a controlled 12/12 h light/dark cycle, at a temperature of 25°C with free access to food and water. The experimental protocols used in this study were approved by the Institutional Animal Care and Use Committee of the State University of Ceará (UECE), Fortaleza-CE, Brazil, in accordance with international guidelines (NIH publication No. 85-23, revised 1985).Algae Champia feldmannii belongs to the order Rhodymeniales, family Lomentariaceae and was collected in the Pacheco beach of Caucaia, Ceará, Brazil. Algae were classified by Wladimir R. L. Farias, Department of Fishery Engeneering of Federal University of Ceará-Brazil.Erythrocytes Human erythrocytes were obtained from healthy donors at the Hematology Center of the Federal University of Ceará-Brazil.Drugs and Reagents Dextran sulphate, L-carrageenan, Evans blue, zymosan, morphine, cethylpiridinium chloride, sodium acetate, calcium chloride, ketamine, sulphuric acid, papain, 1-9-di-metylene blue (DMB), ethylenodiaminotetracetic acid (EDTA), indomethacin, dexamethasone, pentoxifylline, L-N-nitro-arginine methyl ester (L-NAME), meclyzine and formamide, N-acetyl-N,N,N-trimethylammonium bromide, 1,3-diaminopropane, toluidine blue, agarose gel (Sigma Chemical Co., St. Louis, MO, U.S.A. or SigmaAldrich Chemie, Steinheim, Germany); absolute ethanol, sodium chloride, sodium dodecyl sulfate (SDS) and 2-mer- 907, 60.455-970, Fortaleza-Ceará-Brasil. R...

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“…In active paw anaphylaxis, the edematogenic reaction is manifested early after OVA-administration and the peak effect is maintained during 30 min-1 h which decreases thereafter. It involves IgE antibodies and also biogenic amines [24,25]. Active paw anaphylaxis model has widely been utilized for screening of putative anti-allergic drugs [26].…”

Section: Discussionmentioning

confidence: 99%