Sphagneticola calendulacea (L.) Pruski. Is a crawling evergreen weed. It is been commonly known as Creeping Daisy, Bhringaraja, etc. The plant is been used for the treatment of inflammations, including abscesses, sore throat, coughs and elephantiasis. The leaf extract is also in alopecia. For standardization of this herbal plant, Pharmacognosy is carried out. The leaves of the said plant are studied for the parameters like macroscopy, microscopy, and histochemistry and powder study. It was also investigated for physicochemical, fluorescence and phytochemical analysis. The powder study revealed the presence of anisocytic stomata, palisade tissue, tannin filled cell, starch grains, calcium oxalate crystals, oil globules and different types of trichomes. These results go concurrent with microscopy of leaves. The physicochemical parameters also showed significant results. The phytochemical and histochemical analysis showed the presence phytoconstituents like flavonoids, saponins, anthroquinone glycosides, etc. Thus, these parameters will be useful in authenticating the said plant.