The pharmacodynamics of enoxaparin in percutaneous coronary intervention with precise rapid enoxaparin loading (PEPCI-PRE study)

Abstract

This regimen is well suited for ACS treatment with an invasive strategy, including the rapid transition to early and rescue PCI.

A development of LC–MS method combining ultrafiltration and lyophilization for determination of r-RGD-Hirudin in human serum

“…Among these methods, aPPT and CSA related to 1570-0232/$ -see front matter © 2008 Elsevier B.V. All rights reserved. doi:10.1016/j.jchromb.2008.05.032 Table 1 Quantitative parameter in LC-MS method compared with reported methods to determine Hirudin in vivo LC-MS method ELISA [8] ECT [6] aPTT [4,5] Linearity (r) 0.99 0.86 0.9 Not linear, but hyperbolic LLOQ (ng mL −1 ) 2 5 1 0 0 5 0 Precision (RSD,%) All below 15% Mostly above 15% Specificity Good Poor Fair Analytical period Rapid Tedious level of Thrombin or Prothrombin, are widely used in determination of different kinds of Thrombin inhibitors like heparin, so they lack specificity; the snake venom enzyme Ecarin converts Prothrombin to Meizothrombin which can be rapidly neutralized by Hirudin resulting in a dose-dependent prolongation of the clotting times, but it was also reported that the ECT method can be strongly influenced by the Prothrombin level [11,12]. While ELISA and RIA require a monoclonal antibody, which is of higher cost and hard to prepare, the isotope labeling tracer required cannot be used in human body.…”