The pH-sensitive switchable behavior based on the layer-by-layer films of hemoglobin and Ag nanoparticles

Yu, Chunmei; Wan-yu, JI; Gou, Lili; Bao, Ning; Gu, Hongchen

doi:10.1016/j.elecom.2011.10.012

Cited by 15 publications

(14 citation statements)

References 16 publications

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“…Polyelectrolyte multilayer membranes, such as poly (acrylic acid) [3] and branched poly (ethyleneimine) [4], have been modified on electrodes to electrochemically oxidize glucose due to their unique pH-sensitive characteristics. Recently, we also reported that Hb, as an important redox respiratory protein in red cells, was coupled with nanomaterials to modify electrodes with remarkable response to different pH solution [12]. Our study suggested that the -COOH and -NH 2 groups was critical for pH-sensitive behavior of Hb because of their different responses under varied pH environments.…”

Section: Introductionmentioning

confidence: 73%

“…As the pH-switchable properties of Hb was ascribed to its pI (7.1) [12], the pH-controllable performance of chitosan was attributed to its pKa between 6.2 and 6.6 [13,14]. At the molecular level, amino groups and carboxyl groups in Hb and chitosan are responsible for opposite behaviors toward Fe(CN) 6 3− between pH value of 4.0 and 8.0, (chitosan, Hb).…”

Section: A Study On the Mechanismmentioning

confidence: 97%

“…As a matter of fact, chitosan has been used to provide biocompatible Electronic supplementary material The online version of this article (doi:10.1007/s00604-015-1597-2) contains supplementary material, which is available to authorized users. environment for proteins, such as Hb, for fabrication of biosensors [15,16] because of its biological activity and no toxicity [12][13][14][15][16][17][18][19]. It is believed that the combination of chitosan and Hb would allow the fabrication of pH controllable materials.…”

Section: Introductionmentioning

confidence: 99%

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Glassy carbon electrode modified with gold nanoparticles and hemoglobin in a chitosan matrix for improved pH-switchable sensing of hydrogen peroxide

et al. 2015

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Hemoglobin (Hb) has been demonstrated to endow electrochemical sensors with pH-switchable response because of the presence of carboxyl and amino groups. Hb was deposited in a chitosan matrix on a glassy carbon electrode (GCE) that was previously coated with clustered gold nanoparticles (Au-NPs) by electrodeposition. The switching behavior is active ("on") to the negatively charged probe [Fe(CN) 63− ] at pH 4.0, but inactive ("off") to the probe at pH 8.0. This switch is fully reversible by simply changing the pH value of the solution and can be applied for pH-controlled reversible electrochemical reduction of H 2 O 2 catalyzed by Hb. The modified electrode was tested for its response to the different electroactive probes. The response to these species strongly depends on pH which was cycled between 4 and 8. The effect is also attributed to the presence of pH dependent charges on the surface of the electrode which resulted in either electrostatic attraction or repulsion of the electroactive probes. The presence of Hb, in turn, enhances the pH-controllable response, and the electrodeposited Au-NPs improve the capability of switching. This study reveals the potential of protein based pH-switchable materials and also provides a simple and effective strategy for fabrication of switchable chemical sensors as exemplified in a pH-controllable electrode for hydrogen peroxide.

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Section: Introductionmentioning

confidence: 73%

Section: A Study On the Mechanismmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 99%

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Glassy carbon electrode modified with gold nanoparticles and hemoglobin in a chitosan matrix for improved pH-switchable sensing of hydrogen peroxide

et al. 2015

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“…Nanoparticles were used in a recent study [103] to create pH-sensitive switchable interfaces based on a layer-by-layer film formation of haemoglobin. Smart interfaces were created which are switchable between the state 'on' and 'off' controlled by the pH value of the solution.…”

Section: Microgels Hydrogels and Other Carrier Materialsmentioning

confidence: 99%

Smart and green interfaces: From single bubbles/drops to industrial environmental and biomedical applications

Dutschk

Karapantsios

Liggieri

et al. 2014

Advances in Colloid and Interface Science

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Interfaces can be called Smart and Green (S&G) when tailored such that the required technologies can be implemented with high efficiency, adaptability and selectivity. At the same time they have also to be eco-friendly, i.e. products must be biodegradable, reusable or simply more durable. Bubble and drop interfaces are in many of these smart technologies the fundamental entities and help develop smart products of the everyday life.Significant improvements of these processes and products can be achieved by implementing and manipulating specific properties of these interfaces in a simple and smart way, in order to accomplish specific tasks. The severe environmental issues require in addition attributing ecofriendly features to these interfaces, by incorporating innovative , or, sometime, recycle materials and conceiving new production processes which minimize the use of natural resources and energy. Such concept can be extended to include important societal challenges related to support a sustainable development and a healthy population.The achievement of such ambitious targets requires the technology research to be supported by a robust development of theoretical and experimental tools, needed to understand in more details the behavior of complex interfaces. A wide but not exhaustive review of recent work concerned with green and smart interfaces is presented, addressing different scientific and technological fields. The presented approaches reveal a huge potential in relation to various technological fields, such as nanotechnologies, biotechnologies, medical diagnostics, new or improved materials.

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“…Proteins, which are inherently biocompatible in a biological body, have been used for fabricating various electrochemical biosensors [15,16]. Especially, redox proteins including hemoglobin, cytochrome c, and myoglobin have been extensively applied in the construction of electrochemical biosensors, which is ascribed to the electroactivity of redox proteins [10,16].…”

Section: Introductionmentioning

confidence: 99%

pH-Controllable Bioelectrocatalysis Based on Surface Charge and Conformation Switching of Nonelectroactive Protein

Wu¹

2018

International Journal of Electrochemical Science

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Lysozyme (Lys), a nonelectroactive protein, was covalently bonded with L-cysteine (L-Cys) selfassembled monolayers (SAMs) on Au electrode to obtain the pH-switchable interface (Lys/L-Cys). Based on the surface charge and conformational switching of Lys at pH 6.19 and 12.13, the Lys/LCys/Au electrode exhibited a pH-sensitive onoff behavior toward the electroactive probe of Fe(CN) 6 3 . As pH was lower or higher than the isoelectronic point (pI 11.0) of Lys, Lys on electrode was positively or negatively charged by the protonation or deprotonation of amino acid residues. Furthermore, protein conformation was reversibly folded or unfolded to the native state and unfolded intermediate accordingly. Consequently, the electrostatic force between Lys and probe of Fe(CN) 6 3 and reversible conformational change of Lys are responsible for the pH-sensitive onoff behavior of Lys/L-Cys/Au interface. Moreover, the switchable properties of Lys/L-Cys/Au electrode were used to realize pH-controlled electrochemical reduction of H 2 O 2 and exhibited high sensitivity for the bioelectrocatalytic reduction of H 2 O 2 . In the linear range of 0.211.2 mM, the response sensitivity was calculated to be 379 A mM 1 cm 2 . The investigations in the mechanism for pH-sensitive behavior and electrocatalysis of the interface suggest that the nonelectroactive Lys/L-Cys film provides potential for fabricating novel switchable electrochemical biosensors and bioelectronic devices.

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The pH-sensitive switchable behavior based on the layer-by-layer films of hemoglobin and Ag nanoparticles

Cited by 15 publications

References 16 publications

Glassy carbon electrode modified with gold nanoparticles and hemoglobin in a chitosan matrix for improved pH-switchable sensing of hydrogen peroxide

Glassy carbon electrode modified with gold nanoparticles and hemoglobin in a chitosan matrix for improved pH-switchable sensing of hydrogen peroxide

Smart and green interfaces: From single bubbles/drops to industrial environmental and biomedical applications

pH-Controllable Bioelectrocatalysis Based on Surface Charge and Conformation Switching of Nonelectroactive Protein

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