The pesticide Lindane induces dose-dependent damage to granulosa cells in an in vitro culture

Palmerini, Maria Grazia; Zhurabekova, Gulmira; Balmagambetova, Aru; Nottola, Stefania Annarita; Miglietta, Selenia; Belli, Manuel; Bianchi, Serena; Cecconi, Sandra; Nisio, Valentina Di; Familiari, Giuseppe; Macchiarelli, Guido

doi:10.1016/j.repbio.2017.09.008

Cited by 20 publications

(16 citation statements)

References 47 publications

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“…The anatomy course represents for this type of student population the first daunting challenge in their degree curricula. Indeed, anatomical and more widely the morphological sciences provide a great amount of information on the ultrastructure of tissues and organs, and on their topographical and functional relationship [17,18]. This suggests a double challenge: the professor has to wisely select the topics and the quantity of information to deliver; the nursing students have to struggle in the new learning process with a consequently high rate of exams failure.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of Effectiveness of Digital Technologies During Anatomy Learning in Nursing School

et al. 2020

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The disciplines of biosciences included in the curricula of a nursing degree represent a daunting but crucial type of knowledge that a well-prepared nurse should acquire. Given the importance and the objective difficulties of these courses, nursing students experience anxiety, especially for the anatomy course. This anxiety and the related rate of exam failures lead professors to analyze their teaching approach, by diversifying the lecturing methods. The aim of our study was to test the use of a virtual dissection table (DT) during the anatomy lectures of a nursing course, evaluating the anxiety level before the exam and evaluating the exam score. The feedback of the evaluated student population was positive overall. The integration of the DT in anatomy lectures improved the learning performance and mostly enhanced the self-confidence of the first year nursing students.

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Section: Discussionmentioning

confidence: 99%

Evaluation of Effectiveness of Digital Technologies During Anatomy Learning in Nursing School

et al. 2020

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“…GCs were then transferred into culture dishes for IVC. To assess the reproductive toxicity of mancozeb, GCs were exposed or not (control) to increasing concentrations (0.001, 0.01, 0.1, and 1 µg/ml), as previously reported [ 10 , 12 ].…”

Section: Methodsmentioning

confidence: 99%

“…GCs were cultured in DMEM (Dulbecco’s modified Eagle’s medium, GE Healthcare, Little Chalfont, Buckinghamshire, UK) containing 5% FBS (fetal bovine serum) supplemented with 2 mM L-glutamine and antibiotics (100 mM penicillin and 100 µg/ml streptomycin) in 35-mm tissue culture-treated culture dishes for 12 h to allow adhesion [ 10 , 12 ]. Mancozeb (PESTANAL ® , 45553) was dissolved in DMEM to obtain the desired concentrations.…”

Section: Methodsmentioning

confidence: 99%

“…We therefore aimed to ascertain whether the biomolecular alterations and cell reshaping observed following mancozeb exposure were connected to ultrastructural damages and cell-to-cell uncoupling, with subsequent isolation and cell death. We used a model of reproductive toxicity previously described in a study of the hexachlorocyclohexane lindane [ 12 ], comprising mouse GCs cultured in vitro in the presence or absence of increasing concentrations of mancozeb. Ultrastructural analysis of GCs was performed by transmission (TEM) and scanning (SEM) electron microscopy.…”

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Mancozeb impairs the ultrastructure of mouse granulosa cells in a dose-dependent manner

Palmerini

Belli

Nottola

et al. 2018

Journal of Reproduction and Development

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Mancozeb, an ethylene bis-dithiocarbamate, is widely used as a fungicide and exerts reproductive toxicity in vivo and in vitro in mouse oocytes by altering spindle morphology and impairing the ability to fertilize. Mancozeb also induces a premalignant status in mouse granulosa cells (GCs) cultured in vitro, as indicated by decreased p53 expression and tenuous oxidative stress. However, the presence and extent of ultrastructural alterations induced by mancozeb on GCs in vitro have not yet been reported. Using an in vitro model of reproductive toxicity, comprising parietal GCs from mouse antral follicles cultured with increasing concentrations of mancozeb (0.001–1 µg/ml), we sought to ascertain the in vitro ultrastructural cell toxicity by means of transmission (TEM) and scanning (SEM) electron microscopy. The results showed a dose-dependent toxicity of mancozeb on mouse GCs. Ultrastructural data showed intercellular contact alterations, nuclear membrane irregularities, and chromatin marginalization at lower concentrations, and showed chromatin condensation, membrane blebbing, and cytoplasmic vacuolization at higher concentrations. Morphometric analysis evidenced a reduction of mitochondrial length in GCs exposed to mancozeb 0.01−1 µg/ml and a dose-dependent increase of vacuole dimension. In conclusion, mancozeb induced dose-dependent toxicity against GCs in vitro, including ultrastructural signs of cell degeneration compatible with apoptosis, likely due to the toxic breakdown product ethylenethiourea. These alterations may represent a major cause of reduced/delayed/missed oocyte maturation in cases of infertility associated with exposure to pesticides.

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“…Pesticides may also exert a pivotal role in compromising female infertility. For example, mancozeb and lindane, two endocrine disruptors widely used in agriculture, are able to dramatically jeopardize the production of fertilizable oocytes [19][20][21][22][23].…”

Section: Environment-related Infertilitymentioning

confidence: 99%

Technologies for the Production of Fertilizable Mammalian Oocytes

et al. 2019

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Women affected by ovarian pathologies or with cancer can usually preserve fertility by egg/embryo freezing. When oocyte retrieval is not feasible, the only option available is ovarian tissue cryopreservation and transplantation. The culture of follicles isolated from fresh or cryopreserved ovaries is considered still experimental, although this procedure is considered safer, because the risk of unintentional spreading of cancer cells eventually present in cryopreserved tissue is avoided. Animal and human small follicles can be cultured in vitro, but standardized protocols able to produce in vitro grown oocytes with the same developmental capacity of in vivo grown oocytes are not available yet. In fact, the different sizes of follicles and oocytes, the hormonal differences existing between mono- (e.g., human, goat, cow, and sheep) and poly-ovulatory (rodents and pig) species, and the incomplete identification of the mechanisms regulating the oocyte–follicle and follicle–ovary interrelationships affect the outcome of in vitro culture. From all these attempts, however, new ideas arise, and the goal of assuring the preservation of female reproductive potential appears a more realistic possibility. This review surveys and discusses advances and challenges of these technologies that, starting from a simple attempt, are now approaching the biosynthesis of a functional engineered ovary.

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The pesticide Lindane induces dose-dependent damage to granulosa cells in an in vitro culture

Cited by 20 publications

References 47 publications

Evaluation of Effectiveness of Digital Technologies During Anatomy Learning in Nursing School

Evaluation of Effectiveness of Digital Technologies During Anatomy Learning in Nursing School

Mancozeb impairs the ultrastructure of mouse granulosa cells in a dose-dependent manner

Technologies for the Production of Fertilizable Mammalian Oocytes

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