The Perspectives of New Methods in Microcirculation Research*

Messmer, K.; Funk, W.; Endrich, B; Zeintl, H.

doi:10.1159/000410708

Cited by 18 publications

(13 citation statements)

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“…In spite of the fact that the use of the intravital videomicroscope has become increasingly popular for studying organ microcirculation [3,5,6,7,8,9,10,11,12] very few reports in the literature deal with the intravital videomicroscopic evaluation of urinary bladder microcirculation. Our aim was to develop a reproducible rat model to precisely characterize the microvascular perfusion parameters of the urinary bladder in a standardized basic state, using a high quality fluorescence videomicroscope and the well established epi-illumination technique.…”

Section: Discussionmentioning

confidence: 99%

“…A large amount of data exists on the microcirculation of other organs in various species [3,5,6,7,8,9,10,11,12]. Among these, the microcirculatory parameters of the intestine and the mesentery are most suitable for a comparison to our results.…”

Section: Discussionmentioning

confidence: 99%

“…Over the last decade, and in particular over the last 5-7 years, considerable data have been accumulated in this area, primarily from various fields of animal research, using, for instance, fluorescence videomicroscopy [11,12]. The microcirculation of many organs has been observed in vivo under physiological and pathophysiological conditions [5,6,7,8,9,10,11,12], new perspectives for tumor and tissue transplantation research have been opened using fluorescence microscopy [12] and microcirculatory observations of conscious animals has become possible [3,12]. Most of the characteristic details of the microcirculatory response to various pathophysiological stimuli of the urogenital tract, however, remain to be elucidated.…”

Section: Introductionmentioning

confidence: 99%

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New method: the intravital videomicroscopic characteristics of the microcirculation of the urinary bladder in rats

Bajory

Hutter

Krombach

et al. 2002

Urological Research

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Intravital fluorescence microscopy (IVM) is a widely used method to study the microcirculation in several organs. Our aim was to develop a standard rat model to evaluate the microcirculatory characteristics of the urinary bladder under physiological pressure conditions using the most advanced fluorescence videomicroscopic techniques. Spraque-Dawley rats were used after filling their bladders with a constant volume of saline solution. The intravesical pressure was continuously monitored. The bladder was positioned on a specially designed stage and IVM measurements were made at the beginning, the 90th, 120th and 180th min. Arteriolar and venular diameters, functional capillary density, venular red blood cell velocity, arteriolar and venular macromolecular leakage and leukocyte-endothelial cell interactions (observation of rolling and firmly adherent leukocytes) were quantitatively assessed by a computer assisted analysis system. Neither microcirculatory parameters nor the intravesical pressure changed significantly during the observation period of 180 min using constant filling volume. We successfully established a new, well functioning and reproducible model to study the microcirculation of the rat bladder using intravital fluorescent microscopy.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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New method: the intravital videomicroscopic characteristics of the microcirculation of the urinary bladder in rats

Bajory

Hutter

Krombach

et al. 2002

Urological Research

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“…The microscopic images were recorded by a charge-coupled device video camera (FK 6990, Pieper GmbH, Schwerte, Germany) attached to an S-VHS video recorder (BR-S920E, JVC, Tokyo, Japan). 9,10 …”

Section: Intravital Videomicroscopymentioning

confidence: 99%

“…11,12 The venular and arteriolar diameters, venular red blood cell velocity, functional capillary density (FCD) or length of erythrocyte-perfused capillaries per observation area, and macromolecular leakage (extravascular/intravascular fluorescence intensity ratio) were determined within five observation fields. 10 The numbers of rolling and adherent leukocytes were evaluated as a measure of the leukocyte-endothelial cell interaction 12 in five venular segments per animal. Rolling leukocytes were defined as cells moving significantly slower than red blood cells in the centerline of the vessel and are given as the number of rolling leukocytes per vessel diameter per second.…”

Section: Video Analysismentioning

confidence: 99%

Microcirculation of the urinary bladder in a rat model of ischemia-reperfusion-induced cystitis

Bajory

Hutter

Krombach

et al. 2002

Urology

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Objectives. To determine the microcirculatory disturbances in a rat model of ischemia-reperfusion-induced cystitis using intravital fluorescence videomicroscopy. Methods. Twenty male Sprague-Dawley rats were used for the experiments. In 10 animals, warm ischemia of the bladder was induced for 60 minutes. After 30 minutes of reperfusion, microvascular macromolecular leakage, leukocyte-endothelial cell interactions, venular red blood cell velocity, functional capillary density, and the arteriolar and venular diameters were determined by intravital videomicroscopy. In addition, the intravesical pressure and macrohemodynamic parameters were assessed during the experiments. Shamoperated animals served as the controls (n ϭ 10). Results. After ischemia-reperfusion, the numbers of rolling and firmly adherent leukocytes in the postcapillary venules were significantly increased. Venular red blood cell velocity and functional capillary density, as well as the arteriolar and venular diameters, were significantly decreased. The macromolecular leakage had increased in both arterioles and venules. Conclusions. After ischemia-reperfusion, inflammatory reactions and microcirculatory failure were observed in the urinary bladder. This study targeted the microcirculatory consequences of cystitis using intravital videomicroscopy. Because the parameters investigated are relevant not only for ischemia-reperfusion of the urinary bladder but also for cystitis caused by other stimuli, this model represents a novel tool in the field of inflammation research in urology.

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Transport of Macromolecules In Tumor Microcirculation

Jain

1985

Biotechnology Progress

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The Perspectives of New Methods in Microcirculation Research*

Cited by 18 publications

References 0 publications

New method: the intravital videomicroscopic characteristics of the microcirculation of the urinary bladder in rats

New method: the intravital videomicroscopic characteristics of the microcirculation of the urinary bladder in rats

Microcirculation of the urinary bladder in a rat model of ischemia-reperfusion-induced cystitis

Transport of Macromolecules In Tumor Microcirculation

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