The peroxisomal SspA protein is redundant for purine utilization but essential for peroxisome localization in septal pores in Aspergillus nidulans

Dimou, Sofia; Kourkoulou, Anezia; Amillis, Sotiris; Percudani, Riccardo; Diallinas, George

doi:10.1016/j.fgb.2019.103259

Cited by 3 publications

(5 citation statements)

References 42 publications

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“…The formation of Woronin bodies in A. nidulans is largely similar to that of N. crassa , as they also mature from peroxisomes following the import of HexA and SspA ( Figure 1A ) ( Beck and Ebel, 2013 ; Dimou et al. , 2019 ).…”

Section: Resultsmentioning

confidence: 73%

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Woronin body hitchhiking on early endosomes is dispensable for septal localization in Aspergillus nidulans

Songster

Bhuyan

Christensen

et al. 2023

MBoC

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The proper functioning of organelles depends on their intracellular localization, mediated by motor protein-dependent transport on cytoskeletal tracks. Rather than directly associating with a motor protein, peroxisomes move by hitchhiking on motile early endosomes in the filamentous fungus Aspergillus nidulans. However, the physiological role of peroxisome hitchhiking is unclear. Peroxisome hitchhiking requires the protein PxdA, which is conserved within the fungal subphylum Pezizomycotina, but absent from other fungal clades. Woronin bodies are specialized peroxisomes that are also unique to the Pezizomycotina. In these fungi, multinucleate hyphal segments are separated by incomplete cell walls called septa that possess a central pore enabling cytoplasmic exchange. Upon damage to a hyphal segment, Woronin bodies plug septal pores to prevent widespread leakage. Here, we tested if peroxisome hitchhiking is important for Woronin body motility, distribution, and function in A. nidulans. We show that Woronin body proteins are present within all motile peroxisomes and hitchhike on PxdA-labeled early endosomes during bidirectional, long-distance movements. Loss of peroxisome hitchhiking significantly affected Woronin body distribution and motility in the cytoplasm, but Woronin body hitchhiking is ultimately dispensable for septal localization and plugging. [Media: see text] [Media: see text]

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Section: Resultsmentioning

confidence: 73%

“…Mutants that lack the ability to optimally localize Woronin bodies at septa, or at cortical tethers in species like N. crassa , are unable to immediately occlude septal pores following injury ( Liu et al. , 2008 ; Dimou et al. , 2019 ).…”

Section: Resultsmentioning

confidence: 99%

Woronin body hitchhiking on early endosomes is dispensable for septal localization in Aspergillus nidulans

Songster

Bhuyan

Christensen

et al. 2023

MBoC

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“…The formation of Woronin bodies in A. nidulans is largely similar to that of N. crassa, as they also mature from peroxisomes following the import of HexA and SspA (Fig. 2A) 25,26 . To characterize the colocalization of Woronin bodies with peroxisomes in A. nidulans, we quantified the degree of overlap between endogenously tagged HexA, SspA, and the peroxisome marker PTS1 via fluorescence microscopy in germlings.…”

Section: Characterization Of Woronin Body Biogenesis and Distribution...mentioning

confidence: 72%

“…This study shows that Woronin bodies move dynamically and bidirectionally in the hyphal tip by hitchhiking on PxdA-labeled early endosomes in A. nidulans . Though Woronin bodies have been described to move in some fungi ( Z. tritici 33 ; A. fumigatus 19 ; A. nidulans 26 ), their motility has not been well characterized and the mechanism by which they move was previously unknown. We showed that Woronin bodies and peroxisomes often coexist as connected organelles in the cytoplasm, where they hitchhike together on early endosomes via PxdA with no directional bias (Fig.…”

Section: Concluding Thoughtsmentioning

confidence: 99%

Woronin bodies move dynamically and bidirectionally by hitchhiking on early endosomes inAspergillus nidulans

Songster¹,

Bhuyan

Christensen

et al. 2023

Preprint

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The proper functioning of organelles depends on their intracellular localization, mediated by motor protein-dependent transport on cytoskeletal tracks. Rather than directly associating with a motor protein, peroxisomes move by hitchhiking on motile early endosomes in the filamentous fungusAspergillus nidulans. However, the cellular function of peroxisome hitchhiking is unclear. Peroxisome hitchhiking requires the protein PxdA, which is conserved within the fungal subphylum Pezizomycotina, but absent from other fungal clades. Woronin bodies are specialized peroxisomes that are also unique to the Pezizomycotina. In these fungi, multinucleate hyphal segments are separated by incomplete cell walls called septa that possess a central pore enabling cytoplasmic exchange. Upon damage to a hyphal segment, Woronin bodies plug septal pores to prevent widespread leakage. Here, we tested if peroxisome hitchhiking is important for Woronin body motility, distribution, and function inA. nidulans. We show that Woronin body proteins are present within all motile peroxisomes and hitchhike on PxdA-labeled early endosomes during bidirectional, long-distance movements. Loss of peroxisome hitchhiking by knocking out pxdA significantly affected Woronin body distribution and motility in the cytoplasm, but Woronin body hitchhiking is ultimately dispensable for septal localization and plugging.

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“…Within this genus, A. nidulans has received widespread recognition as a model eukaryote. Recognized by Pontecorvo in the late 1940s as an excellent candidate for genetic experiments (Pontecorvo et al, 1953), A. nidulans has been the subject of thousands of research papers (>5000 publications in Pubmed), significantly advancing our understanding of cellular physiology and development, enzymology, chromatin structure, DNA repair, recombination, cell biology, primary metabolism and metabolic pathway regulation (Chemudupati et al, 2019;Dimou et al, 2019;Horio et al, 2019;Marcos et al, 2020;Németh et al, 2019). This organism is also the premier model for exploring fungal secondary metabolism.…”

Section: Introductionmentioning

confidence: 99%

In the fungus where it happens: History and future propelling Aspergillus nidulans as the archetype of natural products research

Caesar

Kelleher

Keller

2020

Fungal Genetics and Biology

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The peroxisomal SspA protein is redundant for purine utilization but essential for peroxisome localization in septal pores in Aspergillus nidulans

Cited by 3 publications

References 42 publications

Woronin body hitchhiking on early endosomes is dispensable for septal localization in Aspergillus nidulans

Woronin body hitchhiking on early endosomes is dispensable for septal localization in Aspergillus nidulans

Woronin bodies move dynamically and bidirectionally by hitchhiking on early endosomes inAspergillus nidulans

In the fungus where it happens: History and future propelling Aspergillus nidulans as the archetype of natural products research

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