The Peroxisomal Membrane Protein Inp2p Is the Peroxisome-Specific Receptor for the Myosin V Motor Myo2p of Saccharomyces cerevisiae

Fagarasanu, Andrei; Fagarasanu, Monica; Eitzen, Gary; Aitchison, John D.; Rachubinski, Richard A.

doi:10.1016/j.devcel.2006.04.012

Cited by 127 publications

(158 citation statements)

References 36 publications

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“…In the inheritance of organelles, sporulation is distinctly different from mitotic divisions. During mitosis, polarized actin cables and myosin motors (Pruyne et al 2004) are used to transport into the bud multiple organelles including vacuolar precursors, cortical ER elements, some Golgi elements, peroxisomes, and mitochondria (Hill et al 1996;Simon et al 1997;Rossanese et al 2001;Fehrenbacher et al 2002;Estrada et al 2003;Fagarasanu et al 2006). Because sporulation produces four daughter cells simultaneously rather than a single bud, mechanisms besides vectoral transport of organelles are required.…”

Section: Membrane-cytoskeletal Interactionsmentioning

confidence: 99%

Sporulation in the Budding Yeast Saccharomyces cerevisiae

2011

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In response to nitrogen starvation in the presence of a poor carbon source, diploid cells of the yeast Saccharomyces cerevisiae undergo meiosis and package the haploid nuclei produced in meiosis into spores. The formation of spores requires an unusual cell division event in which daughter cells are formed within the cytoplasm of the mother cell. This process involves the de novo generation of two different cellular structures: novel membrane compartments within the cell cytoplasm that give rise to the spore plasma membrane and an extensive spore wall that protects the spore from environmental insults. This article summarizes what is known about the molecular mechanisms controlling spore assembly with particular attention to how constitutive cellular functions are modified to create novel behaviors during this developmental process. Key regulatory points on the sporulation pathway are also discussed as well as the possible role of sporulation in the natural ecology of S. cerevisiae. TABLE OF CONTENTS Abstract 737Introduction 738

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Section: Membrane-cytoskeletal Interactionsmentioning

confidence: 99%

Sporulation in the Budding Yeast Saccharomyces cerevisiae

2011

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“…This article was published online ahead of print in MBC in Press (http://www.molbiolcell.org/cgi/doi/10.1091/mbc.E08 -09 -0954) on December 30, 2008. Peroxisomes are moved by a complex of Myo2p and the peroxisomal membrane protein, Inp2p (Fagarasanu et al, 2006a). Late-Golgi elements are moved by Myo2p with Ypt11p, a Rab GTPase, and Ret2p, a subunit of the coatomer complex (Rossanese et al, 2001;Arai et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

PTC1Is Required for Vacuole Inheritance and Promotes the Association of the Myosin-V Vacuole-specific Receptor Complex

Jin

Eves

Tang

et al. 2009

MBoC

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Organelle inheritance occurs during cell division. In Saccharomyces cerevisiae, inheritance of the vacuole, and the distribution of mitochondria and cortical endoplasmic reticulum are regulated by Ptc1p, a type 2C protein phosphatase. Here we show that PTC1/VAC10 controls the distribution of additional cargoes moved by a myosin-V motor. These include peroxisomes, secretory vesicles, cargoes of Myo2p, and ASH1 mRNA, a cargo of Myo4p. We find that Ptc1p is required for the proper distribution of both Myo2p and Myo4p. Surprisingly, PTC1 is also required to maintain the steady-state levels of organelle-specific receptors, including Vac17p, Inp2p, and Mmr1p, which attach Myo2p to the vacuole, peroxisomes, and mitochondria, respectively. Furthermore, Vac17p fused to the cargo-binding domain of Myo2p suppressed the vacuole inheritance defect in ptc1⌬ cells. These findings suggest that PTC1 promotes the association of myosin-V with its organelle-specific adaptor proteins. Moreover, these observations suggest that despite the existence of organelle-specific receptors, there is a higher order regulation that coordinates the movement of diverse cellular components. INTRODUCTIONDuring each cell cycle, cytoplasmic organelles are actively distributed between dividing cells to maintain organelle copy number and volume. The yeast Saccharomyces cerevisiae is an excellent model system for studying the spatial and temporal control of organelle inheritance. In yeast, several organelles are transmitted from mother to daughter cells. These include the vacuole, mitochondria, the endoplasmic reticulum (ER), late-Golgi elements and peroxisomes (reviewed in Weisman, 2006).Early in the cell cycle, a portion of each organelle is transported into the emerging bud. The polarized transport of most organelles from the mother to the bud is an active process that requires the actin cytoskeleton, myosin-V motors, and receptor proteins, which physically connect the motor to organelle cargoes (Beach et al., 2000;Yin et al., 2000;Boldogh et al., 2001;Barr, 2002;Bretscher, 2003;Du et al., 2004;Fagarasanu et al., 2006bFagarasanu et al., , 2007Weisman, 2006). Thus, formation of a complex between the motor and receptor protein is important for polarized organelle transport.Similar to yeast, in vertebrates, myosin-V motors move cargoes along the actin cytoskeleton. The best studied cargo of vertebrate myosin-V are melanosomes, which are moved in melanocytes by myosin-Va. Melanosomes attach to myosin-Va through Rab27a and melanophilin (Fukuda and Kuroda, 2002;Wu et al., 2002). Rab27a, through geranylgeranylation, attaches to the melanosome membrane, and melanophilin connects myosin-Va and Rab27a. Myosin-V-based intracellular movement has been analyzed in many other eukaryotes, including frogs, fish, mammals, and plants; plant myosin-XI is the functional homologue of yeast and vertebrate myosin-V ( Kinkema and Schiefelbein, 1994;Provance and Mercer, 1999;Tuma and Gelfand, 1999;Desnos et al., 2007;Li and Nebenfuhr, 2007;Sheets et al., 2007;Shimmen, 2007).Ge...

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“…Numerous functions have been identified for Myo2p. Myo2p plays a role in membrane transport; myo2 mutants have defects in several organelle-trafficking pathways including polarized vesicle secretion (Johnston et al 1991), inheritance of vacuoles (Hill et al 1996;Catlett and Weisman 1998), peroxisomes (Hoepfner et al 2001;Fagarasanu et al 2006), late Golgi (Rossanese et al 2001), mitochondria (Itoh et al 2002;Boldogh et al 2004;Itoh et al 2004), and spindle pole orientation and resultant nuclear segregation (Gundersen and Bretscher 2003).…”

Section: Introductionmentioning

confidence: 99%

Myo2p, a class V myosin in budding yeast, associates with a large ribonucleic acid–protein complex that contains mRNAs and subunits of the RNA-processing body

Chang¹,

Zaarour²,

Reck-Peterson³

et al. 2008

RNA

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Myo2p is an essential class V myosin in budding yeast with several identified functions in organelle trafficking and spindle orientation. The present study demonstrates that Myo2p is a component of a large RNA-containing complex (Myo2p-RNP) that is distinct from polysomes based on sedimentation analysis and lack of ribosomal subunits in the Myo2p-RNP. Microarray analysis of RNAs that coimmunoprecipitate with Myo2p revealed the presence of a large number of mRNAs in this complex. The Myo2p-RNA complex is in part composed of the RNA processing body (P-body) based on coprecipitation with P-body protein subunits and partial colocalization of Myo2p with P-bodies. P-body disassembly is delayed in the motor mutant, myo2-66, indicating that Myo2p may facilitate the release of mRNAs from the P-body.

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The Peroxisomal Membrane Protein Inp2p Is the Peroxisome-Specific Receptor for the Myosin V Motor Myo2p of Saccharomyces cerevisiae

Cited by 127 publications

References 36 publications

Sporulation in the Budding Yeast Saccharomyces cerevisiae

Sporulation in the Budding Yeast Saccharomyces cerevisiae

PTC1Is Required for Vacuole Inheritance and Promotes the Association of the Myosin-V Vacuole-specific Receptor Complex

Myo2p, a class V myosin in budding yeast, associates with a large ribonucleic acid–protein complex that contains mRNAs and subunits of the RNA-processing body

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