The peroxin Pex34p functions with the Pex11 family of peroxisomal divisional proteins to regulate the peroxisome population in yeast

Tower, Robert J.; Fagarasanu, Andrei; Aitchison, John D.; Rachubinski, Richard A.

doi:10.1091/mbc.e11-01-0084

Cited by 55 publications

(59 citation statements)

References 47 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…All of these proteins are known to influence peroxisome abundance (Lay et al, 2006;Thoms and Erdmann, 2005;Tower et al, 2011), so it is attractive to speculate the Pex35 is part of a regulatory unit that controls peroxisome fission, and therefore numbers, in the cell. The effect on Arf1 was particularly intriguing, because ARFs have previously been implicated in peroxisome formation, but their contribution has been poorly established.…”

Section: Discussionmentioning

confidence: 99%

“…Nearly all of the human PEX genes have orthologs in yeasts; however, yeasts have additional PEX genes without obvious homologues in mammals. PEX34 from the yeast Saccharomyces cerevisiae, a recent addition to the growing list of PEX genes, encodes a peroxisomal membrane protein that functions together with the Pex11 family to control peroxisome number (Tower et al, 2011). The Pex11 family in yeast encompasses the orthologous proteins Pex11, Pex25 and Pex27, and is involved in peroxisome division and proliferation (Thoms and Erdmann, 2005).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Pex35 is a regulator of peroxisome abundance

Yofe

Soliman

Chuartzman

et al. 2017

Journal of Cell Science

View full text Add to dashboard Cite

Peroxisomes are cellular organelles with vital functions in lipid, amino acid and redox metabolism. The cellular formation and dynamics of peroxisomes are governed by PEX genes; however, the regulation of peroxisome abundance is still poorly understood. Here, we use a high-content microscopy screen in Saccharomyces cerevisiae to identify new regulators of peroxisome size and abundance. Our screen led to the identification of a previously uncharacterized gene, which we term PEX35, which affects peroxisome abundance. PEX35 encodes a peroxisomal membrane protein, a remote homolog to several curvature-generating human proteins. We systematically characterized the genetic and physical interactome as well as the metabolome of mutants in PEX35, and we found that Pex35 functionally interacts with the vesicle-budding-inducer Arf1. Our results highlight the functional interaction between peroxisomes and the secretory pathway.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Pex35 is a regulator of peroxisome abundance

Yofe

Soliman

Chuartzman

et al. 2017

Journal of Cell Science

View full text Add to dashboard Cite

show abstract

“…One key reason why Y. lipolytica is such a tractable model to study peroxisome biogenesis is because of its ability to efficiently utilize hydrophobic substrates such as oleic acid, which is accompanied by extensive proliferation of peroxisomes. However, growth of S. cerevisiae in oleic acid medium has been shown to result primarily in increased peroxisomal size rather than increased peroxisomal number (Tower et al, 2011). In contrast, Y. lipolytica cells contain greatly enlarged and much more numerous peroxisomes when grown in oleic acid medium as compared to growth in glucose medium (Smith and Rachubinski, 2001;Chang et al, 2009;.…”

Section: Discussionmentioning

confidence: 99%

“…Since then, new PEX genes have been identified (Managadze et al, 2010;Tower et al, 2011), and the number of eukaryotic genome sequencing projects has increased dramatically, allowing for a more complete picture of peroxisomal protein evolution. We therefore revisited the evolution and distribution of the Pex11 protein family by completing a comparative genomics survey using 125 genomes (supplementary material Table S3) spanning the six eukaryotic supergroups (Walker et al, 2011).…”

Section: A Comparative Genomics Survey Of the Pex11 Protein Familymentioning

confidence: 99%

See 1 more Smart Citation

An ancestral role in peroxisome assembly is retained by the divisional peroxin Pex11 in the yeast Yarrowia lipolytica

Chang

Klute

Tower

et al. 2015

Journal of Cell Science

Self Cite

View full text Add to dashboard Cite

The peroxin Pex11 has a recognized role in peroxisome division. Pex11p remodels and elongates peroxisomal membranes prior to the recruitment of dynamin-related GTPases that act in membrane scission to divide peroxisomes. We performed a comprehensive comparative genomics survey to understand the significance of the evolution of the Pex11 protein family in yeast and other eukaryotes. Pex11p is highly conserved and ancestral, and has undergone numerous lineage-specific duplications, whereas other Pex11 protein family members are fungal-specific innovations. Functional characterization of the in-silico-predicted Pex11 protein family members of the yeast Yarrowia lipolytica, i.e. Pex11p, Pex11Cp and Pex11/25p, demonstrated that Pex11Cp and Pex11/25p have a role in the regulation of peroxisome size and number characteristic of Pex11 protein family members. Unexpectedly, deletion of PEX11 in Y. lipolytica produces cells that lack morphologically identifiable peroxisomes, mislocalize peroxisomal matrix proteins and preferentially degrade peroxisomal membrane proteins, i.e. they exhibit the classical pex mutant phenotype, which has not been observed previously in cells deleted for the PEX11 gene. Our results are consistent with an unprecedented role for Pex11p in de novo peroxisome assembly.

show abstract

The role of peroxisomes in xylose alcoholic fermentation in the engineered Saccharomyces cerevisiae

Dzanaeva

Kruk

Ruchała

et al. 2020

Cell Biology International

View full text Add to dashboard Cite

Xylose is a second‐most abounded sugar after glucose in lignocellulosic hydrolysates and should be efficiently fermented for economically viable second‐generation ethanol production. Despite significant progress in metabolic and evolutionary engineering, xylose fermentation rate of recombinant Saccharomyces cerevisiae remains lower than that for glucose. Our recent study demonstrated that peroxisome‐deficient cells of yeast Ogataea polymorpha showed a decrease in ethanol production from xylose. In this work, we have studied the role of peroxisomes in xylose alcoholic fermentation in the engineered xylose‐utilizing strain of S. cerevisiae. It was shown that peroxisome‐less pex3Δ mutant possessed 1.5‐fold decrease of ethanol production from xylose. We hypothesized that peroxisomal catalase Cta1 may have importance for hydrogen peroxide, the important component of reactive oxygen species, detoxification during xylose alcoholic fermentation. It was clearly shown that CTA1 deletion impaired ethanol production from xylose. It was found that enhancing the peroxisome population by modulation the peroxisomal biogenesis by overexpression of PEX34 activates xylose alcoholic fermentation.

show abstract

The peroxin Pex34p functions with the Pex11 family of peroxisomal divisional proteins to regulate the peroxisome population in yeast

Abstract: Pex34p is a novel peroxisomal protein involved in controlling peroxisome abundance in Saccharomyces cerevisiae. Pex34p acts to control peroxisome numbers both alone and in cooperation with the Pex11 protein family of peroxisome divisional proteins.

Cited by 55 publications

References 47 publications

Pex35 is a regulator of peroxisome abundance

Pex35 is a regulator of peroxisome abundance

An ancestral role in peroxisome assembly is retained by the divisional peroxin Pex11 in the yeast Yarrowia lipolytica

The role of peroxisomes in xylose alcoholic fermentation in the engineered Saccharomyces cerevisiae

Contact Info

Product

Resources

About