The permeability transition pore as a pathway for the release of mitochondrial DNA

Protective behavior of tamoxifen against Hg2+-induced toxicity on kidney mitochondria: In vitro and in vivo experiments

“…The tissue was homogenized in 0. 25 were isolated using the conventional centrifugation procedure. Briefly, the homogenate was centrifuged at 1000 × g for 10 min.…”

“…The accumulation of DCF was measured on the basis of an increase in fluorescence at 488 excitation and 530 emission. Mitochondrial DNA was isolated as described by García et al [25]. The genetic material was analyzed in 0.8% agarose gel and visualized by adding ethidium bromide.…”

Mitochondrial DNA fragments released through the permeability transition pore correspond to specific gene size

“…Zorov (1996) proposed the possible transport of nucleic acid through the permeability transition pore, representing one of the functional states of the mitochondrial benzodiazepine receptor. Recently, the mitochondrial transition pore has been proposed as a route for the import of genetic material into plant mitochondria (Koulintchenko et al, 2003) and for mitochondrial DNA release in mammals (Patrushev et al, 2004;García et al, 2005).…”

“…This nonspecific pore with a diameter of 2-3 nm allows the efflux of molecules of a size up to 1500 Da (Zoratti and Szabò, 1995). Recently, we demonstrated that the induction of permeability transition due to oxidative stress and calcium overload, in vitro, promoted the release of mitochondrial DNA fragments through the permeability transition pore (García et al, 2005). The processes of mitochondrial DNA fragmentation can occur due to oxidative damage induced by free radicals, such as superoxide radical (O 2 U− ), contrary to that which occurs with nuclear DNA.…”

On the Opening of an Insensitive Cyclosporin A Non-specific Pore by Phenylarsine Plus Mersalyl

“…The onset of increased permeability is favored by massive Ca 2+ accumulation and the influence of an inducing agent [1,2]. With regard to the latter, several dissimilar reagents have been used to open the pore, i.e., inorganic phosphate [3], heavy metals [4][5][6], thiol selective reagents [7][8][9], oxidants, such as reactive oxygen-derived species [10][11][12], and organic hydroperoxides [13][14][15][16].…”