The peripheral blood transcriptome reflects variations in immunity traits in swine: towards the identification of biomarkers

Mach, Núria; Gao, Yu Tang; Lemonnier, Gaëtan; Lecardonnel, Jérôme; Oswald, Isabelle P.; Estellé, Jordi; Rogel–Gaillard, Claire

doi:10.1186/1471-2164-14-894

Cited by 38 publications

(33 citation statements)

References 61 publications

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“…On the first and eighth days of the experiment, all piglets were immunized by subcutaneous inoculation with 2 mL of anti M. hyopneumoniae vaccine (Stellamune®, Pfizer Animal Health, Rueil‐Malmaison, France) as already described . At weekly time intervals, blood samples were aseptically collected.…”

Section: Methodsmentioning

confidence: 99%

Identification of Signaling Pathways Targeted by the Food Contaminant FB1: Transcriptome and Kinome Analysis of Samples from Pig Liver and Intestine

Régnier

Gourbeyre

Pinton

et al. 2017

Molecular Nutrition Food Res

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Scope Fumonisin B1 (FB1) is a mycotoxin produced by Fusarium species. In mammals, this toxin causes widespread organ‐specific damage; it promotes hepatotoxicity, is immunotoxic, alters intestinal functions etc. Despite its inhibitory effect on de novo ceramide synthesis, its molecular mechanism of action and toxicity is not totally elucidated. Methods and results To explore the mechanism of FB1 toxicity, we analyzed the transcriptome and the kinome of two organs targeted by FB1: the liver and the jejunum. Pigs were fed for 4 weeks a control diet or a FB1‐contaminated diet (10 mg/kg). As expected, FB1‐exposed pigs gained less weight and displayed a higher sphinganine/sphingosine ratio. Comparison of the transcriptomes and the kinomes of treated versus control pigs showed striking differences. Among the disrupted pathways in liver and jejunum, we highlight Protein Kinase B (AKT) / Phosphatase and tensin homolog (PTEN) at the intersection of the FB1‐modulated pathways. Conclusion Most of the effects of FB1 are mediated by the regulation of ceramide level, which influences protein phosphatase 2 (PP2A) and the phosphoinositide 3‐kinase (PI3K)/AKT signaling pathway. This pathway might be a new target to counteract toxic effect of Fumonisin B1, which is one of the most spread food contaminant in the world.

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Section: Methodsmentioning

confidence: 99%

Identification of Signaling Pathways Targeted by the Food Contaminant FB1: Transcriptome and Kinome Analysis of Samples from Pig Liver and Intestine

Régnier

Gourbeyre

Pinton

et al. 2017

Molecular Nutrition Food Res

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“…These types of studies may be of interest to breeding schemes in identifying genetic factors that could confer susceptibility or resistance to certain diseases in pigs (Mellencamp et al, 2008). For example another study by Mach et al (2013) involved pigs vaccinated with inactivated Mycoplasma hyopneumoniae. They then used a microarray platform consisting of 10,010 unique genes, and identified molecular biomarkers including Granulysin (GNLY), Killer Cell Lectin-Like Receptor G1 (KLRG1), Arachidonate 12-Lipoxygenase 12S Type (ALOX12), C-X3-C Motif Chemokine Receptor 1 (CX3CR1) and Ral Guanine Nucleotide Dissociation Stimulator (RALGDS).…”

Section: Molecular Biomarkers In Pigsmentioning

confidence: 99%

Molecular approaches to the diagnosis and monitoring of production diseases in pigs

Giles

Belkhiri

Barrow

et al. 2017

Research in Veterinary Science

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Production disease in pigs is caused by a variety of different pathogens, mainly enteric and respiratory and can result in significant economic loss. Other factors such as stress, poor husbandry and nutrition can also contribute to an animal's susceptibility to disease. Molecular biomarkers of production disease could be of immense value by improving diagnosis and risk analysis to determine best practice with an impact on increased economic output and animal welfare. In addition to the use of multiplex PCR or microarrays to detect individual or mixed pathogens during infection, these technologies can also be used to monitor the host response to infection via gene expression. The patterns of gene expression associated with cellular damage or initiation of the early immune response may indicate the type of pathology and, by extension the types of pathogen involved. Molecular methods can therefore be used to monitor both the presence of a pathogen and the host response to it during production disease. The field of biomarker discovery and implementation is expanding as technologies such as microarrays and next generation sequencing become more common. Whilst a large number of studies have been carried out in human medicine, further work is needed to identify molecular biomarkers in veterinary medicine and in particular those associated with production disease in the pig industry. The pig transcriptome is highly complex and still not fully understood. Further gene expression studies are needed to identify molecular biomarkers which may have predictive value in identifying the environmental, nutritional and other risk factors which are associated with production diseases in pigs.

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“…Several regions of the genome have been found to be associated with variation in antibody production (reviewed in Uddin et al, 2010). However, Mach et al (2013) did not find gene expression to be significantly affected in the blood of pigs with different levels of antibody following vaccination with M. hyopneumoniae.…”

Section: Variation In Susceptibilitymentioning

confidence: 94%

Genomics to benefit livestock production: improving animal health

Plastow

2016

R. Bras. Zootec.

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-The primary principle underlying the application of genomics is that it has the most value for difficult and expensive to measure traits. These traits will differ between species and probably also between markets. Maintenance of health will be one of the biggest challenges for efficient livestock production in the next few decades. This challenge will only increase in the face of demand for animal protein, resistance to existing drugs, and the pressure to reduce the use of antibiotics in agriculture. There is probably genetic variation in susceptibility for all diseases but little has been done to make use of this variation to date. In part this is because it is very difficult as well as expensive to measure this variation. This suggests that genomics should provide one of the ways of tackling the challenge of improving animal health. This paper will discuss the concepts of resistance, variation in susceptibility, and resilience; provide examples and present some recent results in cattle and pigs; and briefly discuss the application of gene editing in relation to disease resistance.

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The peripheral blood transcriptome reflects variations in immunity traits in swine: towards the identification of biomarkers

Cited by 38 publications

References 61 publications

Identification of Signaling Pathways Targeted by the Food Contaminant FB1: Transcriptome and Kinome Analysis of Samples from Pig Liver and Intestine

Identification of Signaling Pathways Targeted by the Food Contaminant FB1: Transcriptome and Kinome Analysis of Samples from Pig Liver and Intestine

Molecular approaches to the diagnosis and monitoring of production diseases in pigs

Genomics to benefit livestock production: improving animal health

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