The Periodontal Pathogen Aggregatibacter Actinomycetemcomitans Deteriorates Ventricular Remodeling After Myocardial Infarction in Mice

Hanatani, Tomoya; Suzuki, Jun–ichi; Ogawa, Masahito; Aoyama, Norio; Kobayashi, Naho; Hirata, Yasunobu; Nagai, Ryozo; Izumi, Yuichi; Isobe, Mitsuaki

doi:10.1536/ihj.53.253

Cited by 18 publications

(14 citation statements)

References 29 publications

(25 reference statements)

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“…We measured the areas with a computer-assisted analyzer (Scion Image Beta 4.0.2). The fibrotic area ratio (fibrotic area/entire area expressed as a percentage) was calculated as described previously (20).…”

Section: Methodsmentioning

confidence: 99%

Periodontal bacteria aggravate experimental autoimmune myocarditis in mice

Ashigaki

Suzuki

Ogawa

et al. 2013

American Journal of Physiology-Heart and Circulatory Physiology

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Periodontitis is one of the most common infections in humans. Recently, published reports assert that periodontitis is associated with cardiovascular disease. Although it is said that viral, bacterial infections and autoimmune diseases may be the cause of myocarditis, the pathogenesis of it remains unclear. The aim of this study was to investigate the influence of a periodontal pathogen on experimental autoimmune myocarditis (EAM). Porphyromonas gingivalis (P.g.), PBS as a control, were injected into the mice. Histopathological and immunohistochemical analyses were performed. We examined heart mRNA levels using quantitative RT-PCR. The anti-P.g. IgG antibody level in plasma samples of the P.g.-injected group significantly increased compared with the PBS-injected group. Histopathological analysis detected that the myocarditis-affected areas and the fibrotic area in the P.g.-injected EAM group significantly increased compared with the PBS-injected EAM group (P < 0.05). Immunohistochemical analysis detected that more CD11b-positive cells were shown in the heart of the P.g.-injected EAM group compared with the PBS EAM-injected group (P < 0.05). Hearts from the P.g.-injected EAM group showed significantly increased expression of monocyte chemoattractant protein-1, IFN-γ, and matrix metalloproteinase-9 (MMP-9) mRNA compared with the hearts from the PBS-injected EAM group (P < 0.05). On day 7, serum levels of IL-6 were significantly enhanced in the P.g.-injected EAM group compared with the PBS-injected EAM group (P < 0.05). These results showed that P.g. injection could deteriorate EAM in mice through CD11b-positive cells, cytokines, and MMP-9 expression.

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Section: Methodsmentioning

confidence: 99%

Periodontal bacteria aggravate experimental autoimmune myocarditis in mice

Ashigaki

Suzuki

Ogawa

et al. 2013

American Journal of Physiology-Heart and Circulatory Physiology

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“…It has been reported that 10% to 15% of the periodontal patients had been linked to CVD. [17][18][19] We previously showed that periodontopathic pathogens deteriorated ventricular remodeling after MI 20,21) and other cardiovascular diseases. [22][23][24][25][26] However, a detailed mechanism is still to be elucidated.…”

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<i>Porphyromonas Gingivalis</i> Elevated High-Mobility Group Box 1 Levels After Myocardial Infarction in Mice

et al. 2017

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SummaryHigh mobility group box 1 (HMGB1) is a nuclear protein released from necrotic cells, inducing inflammatory responses. Epidemiological studies suggested a possible association between periodontitis and cardiovascular diseases (CVDs). Due to tissue damage and necrosis of cardiac cells following myocardial infarction (MI), HMGB1 is released, activating an inflammatory reaction. However, it remains unclear whether periodontitis is also involved in myocardial damage. The purpose of this study was to determine the effect of the periodontal pathogen Porphyromonas gingivalis (P.g.) after MI in mice.C57BL/6J wild type mice in post-MI were inoculated with P.g. in the infected group (P.g.-inoculated MI group) and with phosphate buffer saline (PBS) in the control group (PBS-injected MI group). Plasma samples and twelve tissue samples from mice hearts after MI were obtained. We determined the expression of HMGB1 by ELISA and immunohistochemistry.The level of HMGB1 protein in the P.g.-inoculated MI group was significantly higher than in the PBSinjected MI group on day 5, but not on day 14. Immunohistochemistry analysis revealed that HMGB1 was mainly expressed in cardiomyocytes, immune cells, and vascular endothelial cells in the PBS-injected MI group, while HMGB1 was seen broadly in degenerated cardiomyocytes, extracellular fields, immune cells, and vascular endothelial cells in the P.g.-inoculated MI group. A significant increase in the number of HMGB1 positive cells was observed in the P.g.-inoculated MI group compared to the PBS-injected MI group.Infection with P.g. after MI enhanced myocardial HMGB1 expression. There is a possible relationship between periodontitis and post-infarction myocardial inflammation through HMGB-1.(Int Heart J 2017; 58: 762-768)

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“…5 Infection with P. gingivalis also accelerated neointimal formation after arterial injury in mice. 6 On the other hand, it was demonstrated that A. actinomycetemcomitans infection deteriorated ventricular remodeling after myocardial infarction 7 and pressure overload-induced myocardial hypertrophy. 8 These findings imply a hypothesis that a specific periodontal pathogen induces or promotes some particular cardiovascular diseases and that other pathogens influence different circulatory disorders.…”

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Periodontal condition in Japanese coronary heart disease patients: A comparison between coronary and non‐coronary heart diseases

Aoyama

Kobayashi

Hanatani

et al. 2018

J of Periodontal Research

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Objective The aim of this clinical trial was to assess the relationship between periodontal bacterial burden and coronary heart disease (CHD) in Japanese population. Background Many epidemiological reports suggest that periodontitis is a risk factor for CHD; however, the influence of each periodontal bacterium and periodontal condition in Japanese CHD patients is unclear. Methods We studied 897 patients with cardiovascular diseases in Tokyo Medical and Dental University Hospital from May 2012 to August 2015. The subjects were divided into six groups according to age and the existence of CHD (46‐60 years with CHD (n = 56): Group YC, 61‐70 years with CHD (n = 106): Group MC, over 70 years with CHD (n = 177): Group EC, 46‐60 years without CHD (n = 152): Group YN, 61‐70 years without CHD (n = 216): Group MN, and over 70 years without CHD (n = 190): Group EN). Results We found that the patients in Groups MC and EC had deeper periodontal pocket compared to the patients in Group YN (P < 0.05), although there was no statistical difference of pocket depth between Group YC and Groups MC and EC. Many subjects in Group EC had high anti‐Porphyromonas gingivalis and anti‐Prevotella intermedia antibodies in comparison to Group EN (P < 0.05). The CHD patients generally had worse oral condition than the non‐CHD patients. Elderly with CHD had a higher level of serum anti‐Porphyromonas gingivalis antibody and anti‐Prevotella intermedia antibody than those without CHD. Conclusion Increased periodontal infection was found in Japanese CHD patients compared to non‐CHD patients.

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The Periodontal Pathogen Aggregatibacter Actinomycetemcomitans Deteriorates Ventricular Remodeling After Myocardial Infarction in Mice

Cited by 18 publications

References 29 publications

Periodontal bacteria aggravate experimental autoimmune myocarditis in mice

Periodontal bacteria aggravate experimental autoimmune myocarditis in mice

<i>Porphyromonas Gingivalis</i> Elevated High-Mobility Group Box 1 Levels After Myocardial Infarction in Mice

Periodontal condition in Japanese coronary heart disease patients: A comparison between coronary and non‐coronary heart diseases

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