The performance of pyrite-based autotrophic denitrification column for permeable reactive barrier under natural environment

Xu, Baokun; Shi, Liangsheng; Zhong, Hua; Wang, Kang

doi:10.1016/j.biortech.2019.121763

Cited by 36 publications

(15 citation statements)

References 34 publications

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“…In treatments where acetate was added at a concentration of 480 mg L −1 , nitrate was almost entirely consumed and 100% of the total nitrate was removed on day 2; acetate was replenished on day 8, while nitrate was replenished on day 4, 8, 12, and 16. The nitrate reduction rate increased with the increase of acetate addition, which was in accordance with previous studies in which it was reported that organic carbon could improve particulate pyrite-based denitrification performance [ 6 , 7 ].…”

Section: Resultssupporting

confidence: 91%

“…To remove sulfate from the pyrite surface, the particulate pyrite was rinsed with both tap and ultra-pure water prior to the experiment, then dried at 105 °C in a vacuum-drying oven for 4 h, cooled, and stored in the dark for later use. The particulate pyrite contained 47.09% ( w / w ) Fe, 39.96% S, 10.40% O, 0.969% Si, and 0.279% Al [ 7 ].…”

Section: Methodsmentioning

confidence: 99%

“…Ammonia nitrogen (NH 4 + -N) was measured with the salicylic acid spectrophotometry method using a spectrophotometer (UV2700, Shimadzu, Kyoto, Japan). The total iron (Fe Total ) and ferrous iron (Fe 2+ ) were measured based on 1, 10-phenanthroline spectrophotometry [ 7 ].…”

Section: Methodsmentioning

confidence: 99%

“…Autotrophic denitrifying bacteria require inorganic compounds as electron donors and carbon dioxide (CO₂) or bicarbonate as carbon sources. Among the inorganic compounds studied, the use of pyrite as the electron donor for autotrophic denitrification has recently gained increasing attention [ 5 , 6 , 7 ].…”

Section: Introductionmentioning

confidence: 99%

“…Although a higher probability of autotrophic denitrification was indicated in a pyrite-based denitrification column, the positive correlation between NO 3 − -N removal efficiency and dissolved organic carbon removal [ 7 ] found in a previous study suggested that the role of organic carbon in denitrification should be further explored. All neutrophilic microbial-driven nitrate-dependent iron oxidation bacterial strains isolated to date are mixotrophic, requiring an organic co-substrate for growth [ 22 , 23 ].…”

Section: Introductionmentioning

confidence: 99%

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Pyrite-Based Autotrophic Denitrifying Microorganisms Derived from Paddy Soils: Effects of Organic Co-Substrate Addition

Yang

et al. 2022

IJERPH

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The presence of organic co-substrate in groundwater and soils is inevitable, and much remains to be learned about the roles of organic co-substrates during pyrite-based denitrification. Herein, an organic co-substrate (acetate) was added to a pyrite-based denitrification system, and the impact of the organic co-substrate on the performance and bacterial community of pyrite-based denitrification processes was evaluated. The addition of organic co-substrate at concentrations higher than 48 mg L−1 inhibited pyrite-based autotrophic denitrification, as no sulfate was produced in treatments with high organic co-substrate addition. In contrast, both competition and promotion effects on pyrite-based autotrophic denitrification occurred with organic co-substrate addition at concentrations of 24 and 48 mg L−1. The subsequent validation experiments suggested that competition had a greater influence than promotion when organic co-substrate was added, even at a low concentration. Thiobacillus, a common chemolithoautotrophic sulfur-oxidizing denitrifier, dominated the system with a relative abundance of 13.04% when pyrite served as the sole electron donor. With the addition of organic co-substrate, Pseudomonas became the dominant genus, with 60.82%, 61.34%, 70.37%, 73.44%, and 35.46% abundance at organic matter concentrations of 24, 48, 120, 240, and 480 mg L−1, respectively. These findings provide an important theoretical basis for the cultivation of pyrite-based autotrophic denitrifying microorganisms for nitrate removal in soils and groundwater.

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Section: Resultssupporting

confidence: 91%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Pyrite-Based Autotrophic Denitrifying Microorganisms Derived from Paddy Soils: Effects of Organic Co-Substrate Addition

Yang

et al. 2022

IJERPH

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Effect of hydraulic retention time on performance of autotrophic, heterotrophic, and split‐mixotrophic denitrification systems supported by polycaprolactone/pyrite: Difference and potential explanation

Yuan

Zhu

Guo³

et al. 2022

Water Environment Research

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Biological denitrification is still the most important pathway to purifying nitrate‐containing wastewater. In this study, pyrite (FeS2) and polycaprolactone (PCL) were used as electron donors to construct sole or combined denitrification systems, that is, pyrite‐based autotrophic denitrification (PAD) system, PCL‐supported heterotrophic denitrification (PHD) system, and split‐mixotrophic denitrification system (combined PAD + PHD), all of which were operated under five different hydraulic retention times (HRTs) for 150 days. The results showed that the removal rates (RE) of nitrate (NO3−‐N) and inorganic phosphorus (PO43−‐P) by PAD were 91% and 94%, respectively, but the effluent sulfate (SO42−) concentration was as high as 168.2 mg/L; the removal rate of NO3−‐N by PHD was higher than 99%, but the PO43−‐P could not be removed ideally; the removal rates of NO3−‐N and PO43−‐P by PAD + PHD were higher than 95% and 99%, respectively, and the effluent SO42− concentration was only 7.2 mg/L. Through the analysis of the surface scanning electron microscope (SEM) images of the two kinds of media before and after use, it was found that the coupled mode of PAD + PHD was more favorable for biofilm formation than the sole PAD or PHD process, and the microorganisms in the PAD + PHD mode made more full use of electron donors. Moreover, the biomass of the PAD + PHD mode was lower than that of the PAD or PHD process, but the denitrification efficiency of the coupled mode was more efficient, indicating that the functional microorganisms in the PAD + PHD mode might have a certain synergistic effect. Practitioner Points Removal rates of NO3−, PO43−, and SO42− by PAD were 91%, 94%, and −233%, respectively. Removal rate of NO3− by PHD exceeded 99%, but PO43− could not be removed ideally. Removal rates of NO3−, PO43−, and SO42− by PAD + PHD were 95%, 99%, and 86%, respectively. The coupled mode was more favorable for biofilm formation than the sole PAD or PHD. The coupled mode had lower biomass but got more excellent denitrification efficiency.

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Effect of copper, arsenic and nickel on pyrite-based autotrophic denitrification

2023

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Pyritic minerals generally occur in nature together with other trace metals as impurities, that can be released during the ore oxidation. To investigate the role of such impurities, the presence of copper (Cu(II)), arsenic (As(III)) and nickel (Ni(II)) during pyrite mediated autotrophic denitrification has been explored in this study at 30 °C with a specialized microbial community of denitrifiers as inoculum. The three metal(loid)s were supplemented at an initial concentration of 2, 5, and 7.5 ppm and only Cu(II) had an inhibitory effect on the autotrophic denitrification. The presence of As(III) and Ni(II) enhanced the nitrate removal efficiency with autotrophic denitrification rates between 3.3 [7.5 ppm As(III)] and 1.6 [7.5 ppm Ni(II)] times faster than the experiment without any metal(loid) supplementation. The Cu(II) batches, instead, decreased the denitrification kinetics with 16, 40 and 28% compared to the no-metal(loid) control for the 2, 5 and 7.5 ppm incubations, respectively. The kinetic study revealed that autotrophic denitrification with pyrite as electron donor, also with Cu(II) and Ni(II) additions, fits better a zero-order model, while the As(III) incubation followed first-order kinetic. The investigation of the extracellular polymeric substances content and composition showed more abundance of proteins, fulvic and humic acids in the metal(loid) exposed biomass. Graphical Abstract

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The performance of pyrite-based autotrophic denitrification column for permeable reactive barrier under natural environment

Cited by 36 publications

References 34 publications

Pyrite-Based Autotrophic Denitrifying Microorganisms Derived from Paddy Soils: Effects of Organic Co-Substrate Addition

Pyrite-Based Autotrophic Denitrifying Microorganisms Derived from Paddy Soils: Effects of Organic Co-Substrate Addition

Effect of hydraulic retention time on performance of autotrophic, heterotrophic, and split‐mixotrophic denitrification systems supported by polycaprolactone/pyrite: Difference and potential explanation

Effect of copper, arsenic and nickel on pyrite-based autotrophic denitrification

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