The performance of decellularized adipose tissue microcarriers as an inductive substrate for human adipose-derived stem cells

Biomimetic 3D tissue printing for soft tissue regeneration

“…Tissue decellularization involving a combination of physical, chemical, and biological treatment is one strategy that has been considered as an effective method for fabrication of ECM scaffolds [15]. However, the ECM materials harvested and processed from tissues often lack a tailored microgeometry [17] and thus cellular distribution is mainly limited to the surface of ECM scaffolds, with only a fraction of total cells penetrating into the interior regions [18]. Furthermore, when ECM scaffolds are implanted, survival of the infiltrating or seeded cell populations is supported primarily by diffusion of oxygen and nutrients until a supporting vascular network forms [19].…”

Extracellular Matrices Decellularized from Embryonic Stem Cells Maintained Their Structure and Signaling Specificity

“…[12][13][14] Tissue-specific ECMs derived from mesenchymal stem cells (MSCs) directed MSC-lineage specification and augmented tissue regeneration by extending site-specific MSC retention. [13][14][15][16][17] In addition, microcarriers made from MSC-derived ECMs promoted MSC adipogenesis and demonstrated in vivo compatibility, indicating the feasibility of their use in large-scale cell production and cell/ matrix delivery. 14,15,18,19 The ECMs decellularized from ESC cultures provided a permissive microenvironment for tissue remodeling and fibroblast repopulation.…”

“…[13][14][15][16][17] In addition, microcarriers made from MSC-derived ECMs promoted MSC adipogenesis and demonstrated in vivo compatibility, indicating the feasibility of their use in large-scale cell production and cell/ matrix delivery. 14,15,18,19 The ECMs decellularized from ESC cultures provided a permissive microenvironment for tissue remodeling and fibroblast repopulation. 11,12 The decellularized ECMs of ESCs have also been shown to contain ESCsecreted factors such as Lefty, which inhibited the growth and migration of tumor cells.…”

Toward reconstruction of the subcutaneous fat layer with the use of adipose-derived stromal cell–seeded collagen matrices

“…Interestingly, the matrices per se appeared to provide an inductive environment for adipogenesis, as a slight adipogenic differentiation, shown on the cellular and molecular level, took place even without exogenous differentiation stimulus. Similarly, an inductive effect of decellularized matrices on adipogenic differentiation has previously been shown also for decellularized extracellular matrix from adipose tissue [25,26].…”