Abstract. The comparison of symmetrical spiral stream anaerobic bioreactor (SSSAB), to three compartmentalized anaerobic bioreactor (TCAB) and upflow anaerobic sludge blanket reactor (UASB) were carried out, the performance, affecting factors, kinetics reaction and sludge features were studied with the same operational conditions under room temperature. The results showed that: the average COD removal efficiency of SSSAB (88%) was higher than that of TCAB and UASB (80% and 78 %). The first-order kinetic constant of SSSAB was 5.4 d -1 , higher than that of TCAB (3.6 d -1 ) and UASB (2.2 d -1 ). In macro scale, compared with that from TCAB and UASB, the anaerobic granular sludge from SSSAB was clearer, more black and denser. Moreover, the surface of anaerobic granular sludge from SSSAB was rough and full of channels. The total amount of extracellular polymeric substance (EPS) of anaerobic granular sludge from SSSAB was higher than that of TCAB and UASB, which provided conditions for mass transfer between sludge and substrates. The protein (PN)/ polysaccharide (PS) ratio of the sludge from SSSAB was lowest, which might indicate that it had more favorable strength and settling ability. The distribution of flocculability of the sludge from SSSAB was more reasonable, and its fluctuation was smaller compared with that from UASB.