The performance and utility of rapid diagnostic assays for<i>Plasmodium falciparum</i>malaria in a field setting in the Lao People's Democratic Republic

Labbé, Annie‐Claude; Pillai, Dylan R.; Hongvangthong, B.; Vanisaveth, V.; Pomphida, S.; Inkathone, S.; Burgess, Deborah C. Hay; Kain, Kevin C.

doi:10.1080/00034983.2001.11813684

Cited by 5 publications

(6 citation statements)

References 13 publications

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“…They typically provide a visual result within 10 to 20 min. 6 Tests for direct or indirect detection of a range of pathogens (including Trypanosoma cruzi, 7 diphtheria toxin, 8 Plasmodium falciparum, 9 and Hemophilis ducreyi 10 ) have been reported and tested and are available in resource-limited settings. Lateral flow assays have also been used for end point detection of PCR and isothermal amplification products, either from reactions conducted using standard equipment 11−14 or integrated into microfluidic systems.…”

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confidence: 99%

“Paper Machine” for Molecular Diagnostics

2015

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Clinical tests based on primer-initiated amplification of specific nucleic acid sequences achieve high levels of sensitivity and specificity. Despite these desirable characteristics, these tests have not reached their full potential because their complexity and expense limit their usefulness to centralized laboratories. This paper describes a device that integrates sample preparation and loop-mediated isothermal amplification (LAMP) with end point detection using a hand-held UV source and camera phone. The prototype device integrates paper microfluidics (to enable fluid handling) and a multilayer structure, or a "paper machine", that allows a central patterned paper strip to slide in and out of fluidic path and thus allows introduction of sample, wash buffers, amplification master mix, and detection reagents with minimal pipetting, in a hand-held, disposable device intended for point-of-care use in resource-limited environments. This device creates a dynamic seal that prevents evaporation during incubation at 65°C for 1 h. This interval is sufficient to allow a LAMP reaction for the Escherichia coli malB gene to proceed with an analytical sensitivity of 1 doublestranded DNA target copy. Starting with human plasma spiked with whole, live E. coli cells, this paper demonstrates full integration of sample preparation with LAMP amplification and end point detection with a limit of detection of 5 cells. Further, it shows that the method used to prepare sample enables concentration of DNA from sample volumes commonly available from fingerstick blood draw.

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confidence: 99%

“Paper Machine” for Molecular Diagnostics

2015

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“…3) providing women with additional nutritional information and skill training might make them feel more empowered to access the resources they needed to adopt the improved technology. (Dunmade, 2002;Liang et al, 2018;Morse et al, 2020;Mulokozi et al, 2001;Parham et al, 2010;Ritenbaugh et al, 1989;Sanborn and Toure, 1984;Sesan, 2012); and five studies (5/19, 26.3%) have been completed in the Asian region (Khodadadeh et al, 2001;Labbé et al, 2001;Monk, Hall, and Hussain, 1984;Nelson, Sutanto, and Suradana, 1999;Parashari et al, 2000). Another feature that has been retrieved is the research design.…”

Section: Characteristics Of the Selected Studiesmentioning

confidence: 99%

“…This review categorized the selected papers on appropriate technology and global health according to their research designs. The research designs used in selected studies include narrative research (6/ 19, 31.6%) (Dunmade, 2002;Feachem, 1980;Free, 1992;Monk, Hall, and Hussain, 1984;Nabarro and Chinnock, 1988;Ong, 1991), clinical trial research (6/19, 31.6%) (Jeronimo et al, 2014;Labbé et al, 2001;Mulokozi et al, 2001;Nelson, Sutanto, and Suradana, 1999;Parashari et al, 2000;Ritenbaugh et al, 1989;Sanborn and Toure, 1984), case studies (2/ 19, 10.5%) (Parham et al, 2010), randomized control trial research (1/19, 5.3%) (Khodadadeh et al, 2001), qualitative research (1/19, 5.3%) (Sesan, 2012), cross-sectional research (1/19, 5.3%) (Liang et al, 2018), and transdisciplinary research (1/19, 5.3%) (Morse et al, 2020). Additionally, the selected studies' objectives and outcomes were obtained.…”

Section: Characteristics Of the Selected Studiesmentioning

confidence: 99%

“…The studies that were chosen to highlight the following outcomes: performance, usability, and needs. Ten studies illustrated the performance of appropriate technology launched there in research (10/19, 52.6) (Jeronimo et al, 2014;Khodadadeh et al, 2001;Labbé et al, 2001;Liang et al, 2018;Nabarro and Chinnock, 1988;Nelson, Sutanto, and Suradana, 1999;Parashari et al, 2000;Parham et al, 2010;Ritenbaugh et al, 1989;Sanborn and Toure, 1984;Sesan, 2012), four studies included usability as a research objective and measure (4/19, 21.1%) (Dunmade, 2002;Monk, Hall, and Hussain, 1984;Morse et al, 2020;Ong, 1991), two studies joint performance and usability (2/ 19, 10.5%) (Liang et al, 2018;Mulokozi et al, 2001), and two studies indicated the need for appropriate technology (2/ 19, 10.5%) (Free, 1992;Leggat, 1997).…”

Section: Characteristics Of the Selected Studiesmentioning

confidence: 99%

“…These findings were mainly positive in their evaluation of appropriate technology use in low-resource settings. The majority of studies evaluated appropriate technologies as easy to use (Khodadadeh et al, 2001;Labbé et al, 2001;Mulokozi et al, 2001;Nelson, Sutanto, and Suradana, 1999). Numerous studies have demonstrated the cost-effectiveness of appropriate technologies (Leggat, 1997;Parashari et al, 2000;Parham et al, 2010).…”

Section: Evaluation Of Appropriate Technologies Implemented In Developing Countriesmentioning

confidence: 99%

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A Rapid Review of the Use of Appropriate Technology in Global Health

et al. 2021

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The need for appropriate technology in global health has expanded dramatically as the gap between industrialized and developing countries continues to expand. However, there is no collective knowledge of appropriate technology in global health. Thus, this study intends to provide light on the latest developments in the field of appropriate technology in global health and to speculate on future directions. A rapid review, or simplified technique, was used to systematically identify and summarize emerging papers. The search technique used the keywords “global health” and “appropriate technology.” The total number of papers collected from PubMed and Scopus was 427, and 19 articles were thoroughly reviewed for the result section following the research. The study's conclusions included the following: 1) an assessment of appropriate technology adopted in developing countries; and 2) strategies for implementing appropriate technologies in global health. Additionally, we drew lessons and identified problems to serve as a useful guide for future research and development in appropriate technology. This review uncovered a small but valuable level of information about acceptable technology in global health.

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Paper-based nuclease protection assay with on-chip sample pretreatment for point-of-need nucleic acid detection

et al. 2020

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Pathogen detection is crucial for human, animal, and environmental health; crop protection; and biosafety. Current culture-based methods have long turnaround times and lack sensitivity. Nucleic acid amplification tests offer high specificity and sensitivity. However, their cost and complexity remain a significant hurdle to their applications in resource-limited settings. Thus, point-ofneed molecular diagnostic platforms that can be used by minimally trained personnel are needed. The nuclease protection assay (NPA) is a nucleic acid hybridization-based technique that does not rely on amplification, can be paired with other methods to improve specificity, and has the potential to be developed into a point-of-need device. In traditional NPAs, hybridization of an anti-sense probe to the target sequence is followed by single-strand nuclease digestion. The double-stranded target-probe hybrids are protected from nuclease digestion, precipitated, and visualized using autoradiography or other methods. We have developed a paper-based nuclease protection assay (PB-NPA) that can be implemented in field settings as the detection approach requires limited equipment and technical expertise. The PB-NPA uses a lateral flow format to capture the labeled target-probe hybrids onto a nitrocellulose membrane modified with an anti-label antibody. A colorimetric enzyme-substrate pair is used for signal visualization, producing a test line. The nuclease digestion of non-target and mismatched DNA provides high specificity while signal amplification with the reporter enzyme-substrate provides high sensitivity. We have also developed an on-chip sample pretreatment step utilizing chitosan-modified paper to eliminate possible interferents from the reaction and preconcentrate nucleic acids, thereby significantly reducing the need for auxiliary equipment.

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The performance and utility of rapid diagnostic assays forPlasmodium falciparummalaria in a field setting in the Lao People's Democratic Republic

Cited by 5 publications

References 13 publications

“Paper Machine” for Molecular Diagnostics

“Paper Machine” for Molecular Diagnostics

A Rapid Review of the Use of Appropriate Technology in Global Health

Paper-based nuclease protection assay with on-chip sample pretreatment for point-of-need nucleic acid detection

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