The Peptidyl Transferase Center: a Window to the Past

Tirumalai, Madhan R.; Rivas, Mario Iván Domínguez; Tran, Quyen; Fox, George E.

doi:10.1128/mmbr.00104-21

Cited by 29 publications

(33 citation statements)

References 274 publications

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“…uL2 is a universal r-protein and is among the first set of large subunit proteins to be assembled into the ribosome ( 6 , 100 , 101 ). In the assembled ribosome structure, it is in very close proximity to what is considered the oldest part of the ribosome, namely, the Peptidyl Transferase Center (PTC) ( 4 , 102 , 103 ). It is also thought to be ancient in origin ( 104 ).…”

Section: Discussionmentioning

confidence: 99%

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Net Charges of the Ribosomal Proteins of the S10 and spc Clusters of Halophiles Are Inversely Related to the Degree of Halotolerance

Tirumalai

Anane-Bediakoh

Rajesh³

et al. 2021

Microbiol Spectr

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The net charges (at pH 7.4) of the ribosomal proteins (r-proteins) that comprise the S10-spc cluster show an inverse relationship with the halophilicity/halotolerance levels in both bacteria and archaea. In non-halophilic bacteria, the S10-spc cluster r-proteins are generally basic (positively charged), while the rest of the proteomes in these strains are generally acidic.

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Section: Discussionmentioning

confidence: 99%

“…The ribosome is a universal molecular machine comprised of RNA and proteins ( 1 ) which catalyzes coded protein synthesis in all three domains of life ( 2 – 4 ). Thirty-four ribosomal proteins (r-proteins) are universally conserved ( 5 – 9 ).…”

Section: Introductionmentioning

confidence: 99%

Net Charges of the Ribosomal Proteins of the S10 and spc Clusters of Halophiles Are Inversely Related to the Degree of Halotolerance

Tirumalai

Anane-Bediakoh

Rajesh³

et al. 2021

Microbiol Spectr

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“…The catalysis does not occur via nucleobase-mediated catalysis, rather is mediated by 2′-hydroxyl of tRNA [64]. Both these reactions occur in the PTC, known to be made completely of RNA [65].…”

Section: Large Ribozymesmentioning

confidence: 99%

Ribozymes as Therapeutic Agents against Infectious Diseases

Wong¹,

Shahid²,

Tan³

2023

RNA Therapeutics - History, Design, Manufacturing, and Applications

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Ribozymes, also known as RNA enzymes, are catalytic RNA molecules capable of cleaving specific RNA sequences, leading to decreased expression of targeted genes. Recent studies suggest their role in cancer therapeutics, genetic diseases and retroviral infections. This book chapter will focus on ribozymes acting as therapeutic agents against infectious diseases caused by viral and bacterial pathogens. Firstly, we will introduce a brief history of ribozymes and a general overview of ribozymes and their characteristics. Next, different types of ribozymes will be explored regarding their targets and mechanisms of action. After that, ribozymes specific to viral and bacterial infections will be explored. We will briefly discuss the current status of ribozymes as therapeutic agents. Finally, the roadblock and challenges ribozymes face before being developed into therapeutic agents—such as their delivery and efficacy issues—will be discussed.

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“…These studies demonstrated that, despite the ribosomes being constituted by RNAs and proteins, in their catalytic site there is no protein enzyme, but rather, the catalytic reaction is carried out by a ribozyme. The characterization of the catalytic activity of ribosomes as an activity carried out by a ribozyme gave extra support and allowed this molecule to be repositioned with more certainty at the origin of the biological systems ( Belousoff et al, 2010 ; Fox, 2010 ; Tirumalai et al, 2021 ). This fact also paved a new way to consider seriously that the origin of life would have happened in a ribonucleoprotein world.…”

Section: The Origin Of Ribosomes and The Organization Of Fucamentioning

confidence: 99%

RNP-world: The ultimate essence of life is a ribonucleoprotein process

Farías

Prosdocimi

2022

Genet. Mol. Biol.

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The fundamental essence of life is based on process of interaction between nucleic acids and proteins. In a prebiotic world, amino acids, peptides, ions, and other metabolites acted in protobiotic routes at the same time on which RNAs performed catalysis and self-replication. Nevertheless, it was only when nucleic acids and peptides started to interact together in an organized process that life emerged. First, the ignition was sparked with the formation of a Peptidyl Transferase Center (PTC), possibly by concatenation of proto-tRNAs. This molecule that would become the catalytic site of ribosomes started a process of self-organization that gave origin to a protoorganism named FUCA, a ribonucleic ribosomal-like apparatus capable to polymerize amino acids. In that sense, we review hypotheses about the origin and early evolution of the genetic code. Next, populations of open biological systems named progenotes were capable of accumulating and exchanging genetic material, producing the first genomes. Progenotes then evolved in two paths: some presented their own ribosomes and others used available ribosomes in the medium to translate their encoded information. At some point, two different types of organisms emerged from populations of progenotes: the ribosome-encoding organisms (cells) and the capsid-encoding organisms (viruses).

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The Peptidyl Transferase Center: a Window to the Past

Cited by 29 publications

References 274 publications

Net Charges of the Ribosomal Proteins of the S10 and spc Clusters of Halophiles Are Inversely Related to the Degree of Halotolerance

Net Charges of the Ribosomal Proteins of the S10 and spc Clusters of Halophiles Are Inversely Related to the Degree of Halotolerance

Ribozymes as Therapeutic Agents against Infectious Diseases

RNP-world: The ultimate essence of life is a ribonucleoprotein process

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