The peptide-tethered lipid membrane as a biomimetic system to incorporate cytochrome c oxidase in a functionally active form

Recent advances in the electrochemistry and spectroelectrochemistry of membrane proteins

“…Interestingly, further incorporation of cytochrome bo 3 inside the bilayer did not significantly change these values, which suggests that the protein does not create too many defects in the structure of the bilayer (Jeuken et al , 2006 ). In contrast, peptide-tethered lipid bilayers with or without embedded cco exhibited high capacitance values (10 -20 μ F/cm 2 ) since they contained more defects (Naumann et al , 1999 ). This could be due to the relative rigidity of the peptide linker that limits the flexibility of the whole structure (Naumann et al , 2011 ).…”

“…For these reasons, the tethered bilayer lipid membrane (tBLM) methodology was developed. The tether is usually a bifunctional thiol consisting of a lipophilic end-group and hydrophilic spacer (Jenkins et al , 1999 ;Naumann et al , 1999 ;Schiller et al , 2003 ). After modification of the metallic surface with the tether, the lipid membrane incorporating the enzyme can be built by fusion of preformed phosphatidylcholine liposomes.…”

“…After modification of the metallic surface with the tether, the lipid membrane incorporating the enzyme can be built by fusion of preformed phosphatidylcholine liposomes. Several terminal oxidases have been immobilized with this methodology: bovine heart cco (Naumann et al , 1999 ) and cytochrome bo 3 from E. coli (Jeuken et al , 2006, Weiss et al , 2010.…”

Membrane Electrochemistry: Electrochemical Processes in Bilayer Lipid Membrane

“…Steinem et al [68] used different spacer and vesicle fusion technique to form a bilayer membrane with 0.8-1.0 μFcm -2 capacitance. Knoll and co-workers [58,63] were the first to introduce to use oligopeptide sequences as hydrophilic spacers attached to the head group of lipid, resulting in the formation of peptide-tethered bilayers. Cornell and coworkers [29,52,[64][65] were the first using half-membrane spanning tether lipids with benzyl disulphide (DPL) and synthetic archaea analogue full membrane spanning lipids with phytanolychains to stabilize the structure and polyethyleneglycol units as a hydrophilic spacer.…”

Supported lipid bilayers, tethered lipid vesicles, and vesicle fusion investigated using gravimetric, plasmonic, and microscopy techniques

“…To overcome this problem, and to simultaneously provide sufficient space underneath the membrane for studies of the membrane-protein controlled molecular trasnport, various ways to anchor planer lipid membranes via chemical tethers have been developed. [3][4][5] The planar configuration of SLBs and tethered lipid bilayers ͑TLBs͒ is also particularly important when highly oriented samples are required, for example, a͒ in studies of two dimensional protein crystals, 6 lipid domain formation, 7 and lateral diffusivity of anchored biomolecular assemblies. 8 However, in many cases, the aim of using supported lipid assemblies is to combine them with surface-based sensors to study kinetics of biorecognition reactions.…”