The pepper dehydration-responsive homeobox 1, CaDRHB1, plays a positive role in the dehydration response

Lim, Chae Woo; Hong, Eunji; Bae, Young‐Min; Lee, Sung Chul

doi:10.1016/j.envexpbot.2017.11.015

Cited by 10 publications

(15 citation statements)

References 60 publications

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“…Pepper (Capsicum annuum) is one of the most important vegetable crops cultivated globally that has economic value as a spice, medicine, vegetable, and biopesticide (Lim et al, 2018b). Although the demand for peppers is increasing worldwide, their productivity can be limited to varying extents by adverse environmental conditions, such as dehydration, high salinity, and extreme temperatures.…”

Section: Introductionmentioning

confidence: 99%

Differential Functions of Pepper Stress-Associated Proteins in Response to Abiotic Stresses

2021

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Stress-associated proteins (SAPs), a group of zinc-finger-type proteins, have been identified as novel regulators of plant abiotic and biotic stresses. However, although they have been discovered in different plant species, their precise functional roles remain unclear. Here, we identified 14 SAP subfamily genes in the pepper genome. An investigation of the promoter regions of these genes for cis-regulatory elements associated with abiotic stress responses revealed the presence of multiple stress-related elements. Domain and phylogenetic analyses using the corresponding protein sequences revealed that the CaSAP genes can be classified into six groups (I–VI) and sorted into two broad types. Expression levels of the CaSAP genes were found to be differentially induced by low temperature, the dehydration stress, or exogenous abscisic acid. Group II and IV genes were highly induced by the low temperature and dehydration treatments, respectively. Moreover, subcellular localization analysis indicated that the proteins in these two groups are distributed in the nucleus, cytoplasm, and plasma membrane. Among the pepper plants silenced with the three identified group II CaSAP genes, the CA02g10410-silenced plants showed tolerance to low temperature, whereas the CA03g17080-silenced plants were found to have temperature-sensitive phenotypes. Interestingly, group IV CaSAP-silenced pepper plants showed drought-tolerant phenotypes. These findings contribute to a preliminary characterization of CaSAP genes and provide directions for future research on the biological role of CaSAPs in response to different abiotic stresses.

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Section: Introductionmentioning

confidence: 99%

Differential Functions of Pepper Stress-Associated Proteins in Response to Abiotic Stresses

2021

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“…Numerous transcription factors, including HD‐ZIP and bZIP, have been found to undergo post‐translational modification during the responses of plants to hormones and environmental stimuli (Himmelbach et al ., 2002; Hill, 2015; Skelly et al ., 2016; Joo et al ., 2021). In our previous study, we demonstrated that CaDRHB1, a member of the HD‐ZIP I subfamily, functions as a positive modulator for drought tolerance via ABA‐mediated stomatal closure (Lim et al ., 2018). On the basis of these observations, we speculated as to whether CaDRHB1 protein stability is modulated by post‐translational modification in the drought response of pepper plants.…”

Section: Resultsmentioning

confidence: 99%

Pepper SUMO E3 ligase CaDSIZ1 enhances drought tolerance by stabilizing the transcription factor CaDRHB1

2022

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Summary Small ubiquitin‐like modifier (SUMO) conjugation (SUMOylation) is a reversible post‐translational modification associated with protein stability and activity, and modulates hormone signaling and stress responses in plants. Previously, we reported that the pepper dehydration‐responsive homeobox domain transcription factor CaDRHB1 acts as a positive modulator of drought response. Here, we show that CaDRHB1 protein stability is enhanced by SUMO E3 ligase Capsicum annuum DRHB1‐interacting SAP and Miz domain (SIZ1) (CaDSIZ1)‐mediated SUMOylation in response to drought, thereby positively modulating abscisic acid (ABA) signaling and drought responses. Substituting lysine (K) 138 of CaDRHB1 with arginine reduced CaDSIZ1‐mediated SUMOylation, indicating that K138 is the principal site for SUMO conjugation. Virus‐induced silencing of CaDSIZ1 promoted CaDRHB1 degradation, suggesting that CaDSIZ1 is involved in drought‐induced SUMOylation of CaDRHB1. CaDSIZ1 interacted with and facilitated SUMO conjugation of CaDRHB1. CaDRHB1, mainly localized in the nucleus, but also in the cytoplasm in the SUMOylation mimic state, suggesting that SUMOylation of CaDRHB1 promotes its nuclear export, leading to cytoplasmic accumulation. Moreover, CaDSIZ1‐silenced pepper plants were less sensitive to ABA and considerably sensitive to drought stress, whereas CaDSIZ1‐overexpressing plants displayed ABA‐hypersensitive and drought‐tolerant phenotypes. Collectively, our data indicate that CaDSIZ1‐mediated SUMOylation of CaDRHB1 functions in ABA‐mediated drought tolerance.

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“…These genes are involved in early leaf development and lateral branch growth. They also respond to different types of stress (e.g., aluminum stress and drought stress) and play a role in the signal transduction of multiple hormones (Nakata et al, 2012;Alvarez et al, 2015;Lim et al, 2018;Li et al, 2019b;Feng et al, 2020). Previous studies have conducted bioinformatics analysis on the PbKNOX gene family, and the research shows that there are 18 PbKNOX genes in pear.…”

Section: Discussionmentioning

confidence: 99%

Genome-Wide Analysis of KNOX Transcription Factors and Expression Pattern of Dwarf-Related KNOX Genes in Pear

et al. 2022

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KNOTTED1-like homeobox (KNOX) transcription factors (TFs) belonging to the homeobox TF family play important roles in plant growth, development, and responses to abiotic and biotic stress. However, little information is available on KNOX TF in pear (Pyrus). In this study, 19 PbKNOXs TFs were re-identified in pear (Pyrus bretschneideri Rehd.). Phylogenetic analysis revealed that the TFs were clustered into three groups with 10 conserved motifs, some of which were group- or subgroup-specific, implying that they are important for the functions of the KNOX in these clades. PbKNM1 and PbKNM2 are KNM (encodes a MEINOX domain but not a homeodomain) genes identified in pear for the first time. KNOX genes in Pyrus and Malus were closely related, and a collinear relationship among PbKNOX genes in Pyrus and Malus was observed. Analysis of the expression patterns of PbKNOX genes in different tissues, at various growth stages, and in response to abiotic and biotic stress revealed that PbKNOXs are involved in plant growth and development. Our comparative transcriptional analysis of dwarf mutant varieties revealed that genes belonging to class I are highly expressed compared with genes in other classes. Analysis of the expression of PbKNOX genes in the hybrid offspring of vigorous and dwarf varieties revealed that PbKNOX genes were highly expressed in the vigorous offspring and weakly expressed in the dwarf offspring. These findings provide new insight into the function of KNOX TFs in pear and will aid future studies of dwarf fruit trees.

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The pepper dehydration-responsive homeobox 1, CaDRHB1, plays a positive role in the dehydration response

Cited by 10 publications

References 60 publications

Differential Functions of Pepper Stress-Associated Proteins in Response to Abiotic Stresses

Differential Functions of Pepper Stress-Associated Proteins in Response to Abiotic Stresses

Pepper SUMO E3 ligase CaDSIZ1 enhances drought tolerance by stabilizing the transcription factor CaDRHB1

Genome-Wide Analysis of KNOX Transcription Factors and Expression Pattern of Dwarf-Related KNOX Genes in Pear

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