The Pentatricopeptide Repeat Protein SOT5/EMB2279 Is Required for Plastid <i>rpl2</i> and <i>trnK</i> Intron Splicing

Huang, Weihua; Zhu, Yajuan; Wu, Wenjuan; Li, Xuan; Zhang, Delin; Yin, Ping; Huang, Jirong

doi:10.1104/pp.18.00406

Cited by 40 publications

(75 citation statements)

References 67 publications

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“…PPD CDS, amiPPD , PPD2‐VP16 and PPD2‐SRDX were recombined into the pGWB2 (Research Institute of Molecular Genetics, Shimane University) vector for constitutive expression. The artificial miRNA amiPPD was designed using the WMD3 Web MicroRNA Designer (http://wmd3.weigel-world.org/cgi-bin/webapp.cgi) and cloned as described by Huang et al (2018). For C‐terminal GFP fusion, PPD2 was recombined into the pGWB5 vector.…”

Section: Methodsmentioning

confidence: 99%

Arabidopsis PEAPODs function with LIKE HETEROCHROMATIN PROTEIN1 to regulate lateral organ growth

Zhu

Luo

Liu

et al. 2019

JIPB

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In higher plants, lateral organs are usually of determinate growth. It remains largely elusive how the determinate growth is achieved and maintained. Previous reports have shown that Arabidopsis PEAPOD (PPD) proteins suppress proliferation of dispersed meristematic cells partly through a TOPLESS corepressor complex. Here, we identified a new PPD-interacting partner, LIKE HETERO-CHROMATIN PROTEIN1 (LHP1), using the yeast two-hybrid system, and their interaction is mediated by the chromo shadow domain and the Jas domain in LHP1 and PPD2, respectively. Our genetic data demonstrate that the phenotype of ppd2 lhp1 is more similar to lhp1 than to ppd2, indicating epistasis of lhp1 to ppd2. Microarray analysis reveals that PPD2 and LHP1 can regulate expression of a common set of genes directly or indirectly. Consistently, chromatin immunoprecipitation results confirm that PPD2 and LHP1 are coenriched at the promoter region of their targets such as D3-TYPE CYCLINS and HIGH MOBILITY GROUP A, which are upregulated in ppd2, lhp1 and ppd2 lhp1 mutants, and that PPDs mediate repressive histone 3 lysine-27 trimethylation at these loci. Taken together, our data provide evidence that PPD and LHP1 form a corepressor complex that regulates lateral organ growth.

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Section: Methodsmentioning

confidence: 99%

Arabidopsis PEAPODs function with LIKE HETEROCHROMATIN PROTEIN1 to regulate lateral organ growth

Zhu

Luo

Liu

et al. 2019

JIPB

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“…Pentatricopeptide repeat (PPR) proteins form the largest family of proteins known to date that are directly involved in posttranscriptional modification in higher plants. Accumulating data indicate that PPR family members serve diverse functions in posttranscriptional modification, such as RNA editing (Ichinose & Sugita, 2018;Jiang et al, 2018;Xiao et al, 2018a,b), RNA splicing Huang et al, 2018;Tadini et al, 2018), RNA processing (Rackham et al, 2016;Stoll et al, 2017), RNA stability (Hammani et al, 2016;Zoschke et al, 2016;, RNA translation Legen et al, 2018), and RNA maturation (W. . The PPR gene family comprises 450 members in Arabidopsis and 477 members in rice (Saha et al, 2007;Barkan & Small, 2014).…”

Section: Introductionmentioning

confidence: 99%

The RNA editing factor DUA1 is crucial to chloroplast development at low temperature in rice

Cui

Wang

et al. 2018

New Phytologist

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Summary Low temperature stress hinders plant growth and chloroplast development and can limit the geographic range of cultivars. In rice, japonica cultivars have greater chilling tolerance than indica cultivars, but the molecular mechanism underlying chilling tolerance is unclear. Here, we report an RNA‐binding protein, DUA1, cloned from the indica cultivar Dular, which exhibits a deficiency in chloroplast development at an early stage of development under low‐temperature conditions. DUA1 shares high sequence homology with the pentatricopeptide repeat family and functions in plastid RNA editing under low‐temperature conditions. Our data suggest that DUA1 can bind to the plastid‐encoded rps8‐182 transcript and disruption of DUA1 activity impairs editing. The RNA editing cofactor WSP1, a partner of DUA1, also participates in chloroplast development at low temperature. Western blot analysis indicates that WSP1 enhances DUA1 stability under low temperatures. DUA1 sequence analyses of rice core germplasm revealed that three major haplotypes of DUA1 and one haplotype showed substantial differences in chlorophyll content under low‐temperature conditions. Variation at DUA1 may play an important role in the adaptation of rice to different growing regions.

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“…Studies have shown the requirement for several nuclear‐encoded protein factors to facilitate rpl2 group IIA intron excision. These factors include nuclear‐encoded SOT5 and WTF1 (Huang et al, 2018; Kroeger, Watkins, Friso, Wijk, & Barkan, 2009). Importantly, although these same studies indicated additional proteins were involved in splicing of rps12‐ 2, our findings imply that MatK is sufficient for rps12‐2 excision but not sufficient for rpl2 .…”

Section: Discussionmentioning

confidence: 99%

Unraveling the role of the enigmatic MatK maturase in chloroplast group IIA intron excision

2020

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Maturases are prokaryotic enzymes that aid self‐excision of introns in precursor RNAs and have evolutionary ties to the nuclear spliceosome. Both the mitochondria and chloroplast, due to their prokaryotic origin, encode a single intron maturase, MatR for the mitochondria and MatK for the chloroplast. MatK is proposed to aid excision of seven different chloroplast group IIA introns that reside within precursor RNAs for essential elements of chloroplast function. We have developed an in vitro activity assay to test chloroplast group IIA intron excision. Using this assay, we demonstrate self‐excision of the group IIA intron of the second intron of rps12 and the group IIA intron of rpl2. We further show that the addition of heterologously expressed MatK protein increases efficiency of group IIA intron self‐splicing for the second intron of rps12 but not the group IIA intron of rpl2. These data, to our knowledge, provide the first direct evidence of MatK’s maturase activity.

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The Pentatricopeptide Repeat Protein SOT5/EMB2279 Is Required for Plastid rpl2 and trnK Intron Splicing

Cited by 40 publications

References 67 publications

Arabidopsis PEAPODs function with LIKE HETEROCHROMATIN PROTEIN1 to regulate lateral organ growth

Arabidopsis PEAPODs function with LIKE HETEROCHROMATIN PROTEIN1 to regulate lateral organ growth

The RNA editing factor DUA1 is crucial to chloroplast development at low temperature in rice

Unraveling the role of the enigmatic MatK maturase in chloroplast group IIA intron excision

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