The PENGUIN approach to reconstruct protein interactions at enhancer-promoter regions and its application to prostate cancer
Alexandros Armaos,
François Serra,
Iker Núñez-Carpintero
et al.
Abstract:We introduce Promoter-Enhancer-Guided Interaction Networks (PENGUIN), a method for studying protein-protein interaction (PPI) networks within enhancer-promoter interactions. PENGUIN integrates H3K27ac-HiChIP data with tissue-specific PPIs to define enhancer-promoter PPI networks (EPINs). We validated PENGUIN using cancer (LNCaP) and benign (LHSAR) prostate cell lines. Our analysis detected EPIN clusters enriched with the architectural protein CTCF, a regulator of enhancer-promoter interactions. CTCF presence w… Show more
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