The PEI-introduced CS shell/PMMA core nanoparticle for silencing the expression of E6/E7 oncogenes in human cervical cells

Saengkrit, Nattika; Sanitrum, Phakorn; Woramongkolchai, Noppawan; Saesoo, Somsak; Pimpha, Nuttaporn; Chaleawlert-umpon, Saowaluk; Tencomnao, Tewin; Puttipipatkhachorn, Satit

doi:10.1016/j.carbpol.2012.06.079

Cited by 30 publications

(17 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…25 It can be introduced into the cell for knockdown of a gene of interest by using various methods. 26 The technique's medical potential lies in its ability to target particular genes and their protein products with great precision. RNAi can therefore be used to switch off rogue genes, of the sort that drive cancer or other disorders, without messing up the chemistry of healthy cells.…”

Section: Introductionmentioning

confidence: 99%

A Computational Approach to Design Potential Antiviral RNA for 3’UTR Post Transcriptional Gene Silencing of Different Strains of Zika Virus

Hashem¹,

Shuvo²,

Arifuzzaman³

2017

JYP

View full text Add to dashboard Cite

Section: Introductionmentioning

confidence: 99%

A Computational Approach to Design Potential Antiviral RNA for 3’UTR Post Transcriptional Gene Silencing of Different Strains of Zika Virus

Hashem¹,

Shuvo²,

Arifuzzaman³

2017

JYP

View full text Add to dashboard Cite

“…PEI is a branched or linear cationic polymer, with low immunogenic stimulation and safety issues, that is used widely in siRNA delivery (Saengkrit et al 2012). PEI participates in non-covalent reactions with nucleic acid molecules, mediated by a protonable amino group in every third position and high cationic charge density at physiological pH.…”

Section: Introductionmentioning

confidence: 99%

Polyelectrolyte complexes of hTERT siRNA and polyethyleneimine: Effect of degree of PEG grafting on biological and cellular activity

Safari

Tamaddon

Zarghami

et al. 2015

Artificial Cells, Nanomedicine, and Biotechnology

View full text Add to dashboard Cite

Gene silencing by siRNA (short interfering RNA)-targeted human telomerase reverse transcriptase (hTERT) is considered a successful strategy for cancer gene therapy. Polyelectrolyte complexes (PEC) of siRNA and cationic polymers such as polyethyleneimine (PEI) have been widely used for cellular transfection; however, they demonstrate some disadvantages such as cytotoxicity and extracellular matrix restrictions. PEG grafting technology was used in an attempt to improve the biocompatibility of PECs. Considering that this technology may compromise the cellular uptake of PECs, we aimed to study the effect of degree of PEI PEGylation on the carrier cytotoxicity, cellular association, and transfection efficiency of hTERT siRNA in the lung cancer cell line A549. Activated NHS ester of methoxy PEG-COOH 5 KDa was grafted to hyperbranched PEI 25 KDa in the molar ratios of 0.2 and 1. The copolymers were characterized by (1)H-NMR spectroscopy. PECs of PEI or PEG-g-PEI with siRNA, alone or co-incubated with heparin sulfate, were studied by the ethidium bromide exclusion assay. Cytotoxicity of the polymers (PEG-g-PEI vs PEI), alone and upon formation of PEC nanoparticles with hTERT siRNA, was determined by a validated MTT assay, in comparison to a scrambled control sequence, in A549 human lung carcinoma cells. The cellular uptake of the PECs of FITC-labeled siRNA was investigated by flow cytometry at different N/P ratios, and the silencing effect of the transfected siRNA was compared to that of the control sequence for different PECs by real time RT-PCR. The cytotoxicity of PEI decreased significantly by PEG grafting, even at a low degree of PEGylation. Moreover, the nonspecific cytotoxicity of PECs decreased by PEG grafting. PECs of PEG-g-PEI showed more biologic stability on incubation with heparin sulfate. Average particle size and zeta potential of PEC nanoparticles were diminished for those of PEG-g-PEI. The cellular association was more pronounced at an N/P ratio of 2.5 for PECs of PEI and PEG-g-PEI alike. The level of silencing of hTERT mRNA by PEC of PEG-g-PEI was sequence-dependent, and determined non-inferior when compared to the native PEI. Conclusively, the biocompatibility of PEI was improved by a low degree of PEGylation, with no adverse effect on the cellular uptake and the transfection activity. PEC nanoparticles of hTERT siRNA and PEG-g-PEI could act as a promising weapon against A549 cells, which has to be considered for an in vivo evaluation.

show abstract

“…Cervical cancer is a malignant proliferation of epithelial cells in cervix uterine [1]. It is the most common cancer in female genital system.…”

Section: Introductionmentioning

confidence: 99%

Surface modification of tri-calcium phosphate nanoparticles by DOPE and/or anti-E6 antibody to enhance uptake of antisense of E6 mRNA

Jebali

Kalantar

Hekmatimoghaddam

et al. 2015

Colloids and Surfaces B: Biointerfaces

View full text Add to dashboard Cite

The PEI-introduced CS shell/PMMA core nanoparticle for silencing the expression of E6/E7 oncogenes in human cervical cells

Cited by 30 publications

References 33 publications

A Computational Approach to Design Potential Antiviral RNA for 3’UTR Post Transcriptional Gene Silencing of Different Strains of Zika Virus

A Computational Approach to Design Potential Antiviral RNA for 3’UTR Post Transcriptional Gene Silencing of Different Strains of Zika Virus

Polyelectrolyte complexes of hTERT siRNA and polyethyleneimine: Effect of degree of PEG grafting on biological and cellular activity

Surface modification of tri-calcium phosphate nanoparticles by DOPE and/or anti-E6 antibody to enhance uptake of antisense of E6 mRNA

Contact Info

Product

Resources

About