The PEGylated liposomal doxorubicin improves the delivery and therapeutic efficiency of 188Re-Liposome by modulating phagocytosis in C26 murine colon carcinoma tumor model

Hsu, Wei‐Hsin; Liu, Si-Yen; Chang, Ya‐Jen; Chang, Cheng-Yen; Ting, Gann; Lee, Te‐Wei

doi:10.1016/j.nucmedbio.2014.05.142

Cited by 13 publications

(9 citation statements)

References 32 publications

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“…In addition, there was no significant difference in left ventricular function and blood perfusion between the FGF1 solution group and the FGF1-nlip group. The reason is that the drug encapsulated by liposomes alone lacks targeting ability and is easily captured by the reticuloendothelial system in vivo (Ishihara et al, 2012;Hsu et al, 2014). It is difficult to maximize the efficacy of the drug in cardiac aggregation.…”

Section: Discussionmentioning

confidence: 99%

Assessment of the Preventive Effect Against Diabetic Cardiomyopathy of FGF1-Loaded Nanoliposomes Combined With Microbubble Cavitation by Ultrasound

Zheng

Shen

et al. 2020

Front. Pharmacol.

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Acidic fibroblast growth factor (FGF1) has great potential in preventing diabetic cardiomyopathy. This study aimed to evaluate the preventive effect of FGF1-loaded nanoliposomes (FGF1-nlip) combined with ultrasound-targeted microbubble destruction (UTMD) on diabetic cardiomyopathy (DCM) using ultrasound examination. Nanoliposomes encapsulating FGF1 were prepared by reverse phase evaporation. DM model rats were established by intraperitoneal injection of streptozotocin (STZ), and different forms of FGF1 (FGF1 solution, FGF1-nlip, and FGF1-nlip+UTMD) were used for a 12-week intervention. According to the transthoracic echocardiography and velocity vector imaging (VVI) indexes, the LVEF, LVFS, and VVI indexes (Vs, Sr, SRr) in the FGF1-nlip+UTMD group were significantly higher than those in the DM model group and other FGF1 intervention groups. From the realtime myocardial contrast echocardiography (RT-MCE) indexes, the FGF1-nlip+UTMD group A and A×b showed significant differences from the DM model group and other FGF1 intervention groups. Cardiac catheter hemodynamic testing, CD31 immunohistochemical staining, and electron microscopy also confirmed the same conclusion. These results confirmed that the abnormalities, including myocardial dysfunction and perfusion impairment, could be suppressed to different extents by the twice weekly FGF1 treatments for 12 consecutive weeks (free FGF1, FGF1-nlip, and FGF1-nlip+UTMD), with the strongest improvements observed in the FGF1-nlip+UTMD group. In conclusion, the VVI and RT-MCE techniques can detect left ventricular systolic function and perfusion changes in DM rats, providing a more effective experimental basis for the early detection and treatment evaluation of DCM, which is of great significance for the prevention of DCM.

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Section: Discussionmentioning

confidence: 99%

Assessment of the Preventive Effect Against Diabetic Cardiomyopathy of FGF1-Loaded Nanoliposomes Combined With Microbubble Cavitation by Ultrasound

Zheng

Shen

et al. 2020

Front. Pharmacol.

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“…The DOPEPEG2000-oleic acid liposomes were successfully encapsulated more than 60% of the anticancer drug introduced into the formulations. Hydrophilic drugs, such as folinic acid calcium salt and methotrexate were located in the aqueous part of the liposomes while lipophilic drugs, such as doxorubicin and irinotecan hydrochloride were entrapped within the bilayer of the liposomes [3,8,25]. The encapsulation of hydrophilic drugs was lower than hydrophobic drugs due to high solubility of hydrophilic 1.0x10 -9 Release Rate (mg/s) Molar Ratio of DOPEPEG2000 to Oleic Acid Follinic Acid Methotrexate Doxorubicin Irrinotecan Fig.…”

Section: Encapsulation Efficiencymentioning

confidence: 99%

“…Therefore, PEG protects the liposomes and liposomes remain in blood circulation for a longer period of time. The incorporation of PEG bearing lipid molecules can reduce most of the challenges encountered by classical liposome technology (without lipid-PEG) such as reduced toxicity from charged liposomes, the inability to evade interception by the immune system and improved stability [8]. Incorporation of PEG into liposomes surface could also enhance the entrapment efficiency and permeability of liposomes through membrane and retention in the blood system.…”

Section: Introductionmentioning

confidence: 99%

Development and Characterization of DOPEPEG2000 Coated Oleic Acid Liposomes Encapsulating Anticancer Drugs

Suk

Misran

2016

J Surfact & Detergents

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Liposomes have been widely investigated as drug carriers for a broad range of anticancer drugs. However, their application as a drug delivery system may be challenging due to low encapsulation of active ingredient and poor stability which leads to uncontrolled delivery of bioactive ingredients. The present study aims at incorporating 1,2‐dioleoyl‐sn‐glycero‐3‐phosphoethanolamide‐N‐[methoxy(polyethylene glycol)‐2000 (DOPEPEG2000) into oleic acid (OA) liposomes in order to prolong the lifetime in blood circulation. The pH transition curve of oleic was obtained by titration of sodium oleate solution with 0.05 mol dm−3 HCl to determine the region where liposomes were abundantly present. The critical vesicle concentration (CVC) was then determined by surface tension measurements. Dry lipid liposomes were hydrated with phosphate buffer at just over twice the CVC value. Optical polarizing microscopy (OPM) showed the presence of liposomes while transmission electron microscopy (TEM) confirmed that the liposomes prepared were spherical in shape and less than 200 nm in size. The incorporation of DOPEPEG2000 into the oleic acid liposomes reduced the average particle size and zeta potential to mimic the red blood cells. The encapsulation efficiency of various anticancer drugs was more than 60% while more than 20% of the drugs were released after 24 h. The results suggest that DOPEPEG2000: oleic acid at a molar ratio of 1:50 fulfilled the requirements for intravenous drug delivery.

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“…The mixture of these materials was less explored due to the instability of liposomes prepared by the fatty acid or phospholipid alone. However, incorporation of stealth molecule (Hsu et al 2014;Jøraholmen et al 2017;Lehtinen et al 2012;Suzuki et al 2012), (1,2-dioleoylsn-glycerol-3 phosphoethanolamide -N-[methoxy (polyethylene glycol)-2000]) in the formulation enhanced the stability of liposomes (Hsu et al 2014), which expected to provide a protective coating from reticuloendothelial system (RES) and prolong the blood circulation half-life (Lehtinen et al 2012).…”

Section: Introductionmentioning

confidence: 99%

Pegylated Oleic Acid-Lecithin Liposomes (Poll) for Anticancer Drug Delivery

Suk¹,

Chung²,

Misran³

2020

JSM

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Cancer is a major health issue, conferring to more than 14.5 million deaths worldwide. Liposomes, self-assembly amphiphilic bilayer molecules, served as excellent alternative vehicles due to their ability to encapsulate both hydrophobic and hydrophilic anticancer drugs. Conventional liposomes, comprised mainly phospholipids are costineffective, unstable, and easily degraded by the external environment. In this study, we introduced PEGylated oleic acid-lecithin liposomes constructed by using C-18 monounsaturated fatty acids (oleic acid) and soy lecithin, in the presence of DOPEPEG2000 in pH7.4, above their glass transition temperature, T g , by employing the simple thin layer lipid hydration method. FTIR spectrum of oleic acid, soy lecithin, and DOPEPEG2000 was studied. The average particle size without further mechanical interference was 1102.3 nm while the zeta potential value was -18 mV, which is compatible with the zeta potential of the red blood cell. The polydispersity index (PDI) was reduced by 46.2% with the incorporation of the DOPEPEG2000. The morphological study using OPM showed the presence of spherical shape liposomes that exhibit the birefringence effect under the light field and Maltese cross under the dark field. Encapsulation of folinic acid, methotrexate, doxorubicin, or irinotecan resulted in greater than 75% encapsulation efficiency (EE). Half-maximal inhibitory concentration, IC 50 , was significantly reduced in POLL as compared to free anticancer drugs. Our data demonstrate POLL may be a promising alternative vehicle to deliver various anticancer drugs to targeted tumour sites. ABSTRAKBarah merupakan isu kesihatan utama yang menyebabkan lebih 14.5 juta kematian di seluruh dunia. Liposom, iaitu molekul swabentuk dwilapis merupakan penghantar alternatif terbaik berikutan kebolehannya untuk mengkapsulkan ubatan anti barah hidrofobik dan hidrofilik. Liposom yang lazimnya diperbuat daripada fosfolipid adalah tidak efektif daripada segi kos, tidak stabil dan mudah terurai oleh persekitaran luar. Dalam kajian ini, kami memperkenalkan liposom asid olik-lesitin berPEG yang terdiri daripada asid lemak tak tepu C-18 (asid olik) dan lesitin soya, dengan kehadiran DOPEPEG2000 dalam pH7.4, pada suhu lebih tinggi daripada suhu peralihan gelas T g , menggunakan metod hidrasi lapisan nipis lipid. Spektrum FTIR asid olik, lesitin soya, dan DOPEPEG2000 juga turut dikaji. Purata saiz partikel adalah 1102.3 nm manakala keupayaan zeta adalah -18 mV, sesuai dengan keupayaan zeta sel darah merah. Indeks polisebaran telah berkurang sebanyak 46.2% dengan penambahan DOPEPEG2000. Kajian morfologi menggunakan Mikroskop Optikal Pengutuban menunjukkan kewujudan liposom berbentuk sfera yang menghasilkan kesan dwibiasan pada medan cerah dan silang Maltese pada medan gelap. Pengkapsulan asid folinik, metotreksat, doksorubisin atau irinotecan menghasilkan lebih 75% kecekapan pengkapsulan. Kepekatan separa rencatan, IC 50 , telah berkurang dengan signifikan pada POLL berbanding ubatan anti barah. Data menunjukkan POLL mungkin ...

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The PEGylated liposomal doxorubicin improves the delivery and therapeutic efficiency of 188Re-Liposome by modulating phagocytosis in C26 murine colon carcinoma tumor model

Cited by 13 publications

References 32 publications

Assessment of the Preventive Effect Against Diabetic Cardiomyopathy of FGF1-Loaded Nanoliposomes Combined With Microbubble Cavitation by Ultrasound

Assessment of the Preventive Effect Against Diabetic Cardiomyopathy of FGF1-Loaded Nanoliposomes Combined With Microbubble Cavitation by Ultrasound

Development and Characterization of DOPEPEG2000 Coated Oleic Acid Liposomes Encapsulating Anticancer Drugs

Pegylated Oleic Acid-Lecithin Liposomes (Poll) for Anticancer Drug Delivery

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