The peculiar thermo-structural behavior of the anionic lipid DMPG

Lamy, M. Teresa; Riske, Karin A.

doi:10.1016/s0009-3084(02)00175-5

Cited by 91 publications

(121 citation statements)

References 40 publications

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“…2. By eyes, the intermediate region is rather transparent compared to the gel and fluid phases where spherical vesicles with μm-scale yields turbidity [2,4,7,8]. Transparency in the intermediate phase indicates change in surface properties associated with the reflective index of the bilayer membrane or mesoscale transformation of the structure such as a rupture of the vesicle and a ripple phase.…”

Section: Rheometrymentioning

confidence: 99%

“…Here we should remark that the minimum viscosity does not appear at low DMPG concentration. In earlier works, perforated membrane formation due to a repulsive interaction among DMPG head groups was proposed as a model of the intermediate phase [2,4,5]. Especially at low DMPG concentration, the formation of pores in the bilayer membranes expands the radius of gyration of DMPG vesicles in order to accommodate holes [8,12,13].…”

Section: Structural Transition Mechanismmentioning

confidence: 99%

“…DMPG dispersions exhibit an anomalous melting (gel / fluid transition) behavior over a wide ranges of the temperatures, instead of a definite melting transition [2][3][4]. In the gel and fluid phases, DMPG forms spherical vesicles with low viscosity.…”

Section: Introductionmentioning

confidence: 99%

“…In the gel and fluid phases, DMPG forms spherical vesicles with low viscosity. However, in the chain-melting "regime", the system shows specific viscous property, which clearly suggests the existence of an intermediate structure between gel and fluid phases [2,4,5]. The phase behavior of DMPG dispersions in the quiescent state have been intensively studied by differential scanning calorimetry (DSC) and small angle X-ray scattering (SAXS) measurements [2][3][4][5][6][7][8][9][10].…”

Section: Introductionmentioning

confidence: 99%

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Rheological characterization of thermal phase behavior of anionic lipid DMPG dispersions

Fujii

2017

J Biorheol

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We study thermo-structural behavior of anionic lipid 1,2-dimyristoyl-sn-glycero-3-phosphoglycerol (DMPG) dispersed systems. Continuous chain-melting transition of DMPG induces anomalous intermediate phase between gel and fluid phases. We found that the intermediate phase can be divided into two domains; anisotropic phase with high orientational order and isotropic viscous phase. Anisotropic phase would be identified to perforated lamellae or bicelle formed by rupturing the perforated vesicles under shear. Isotropic viscous phase, on the other hand, might be identified to densely packed perforated vesicle dispersions formed by rearranging extended bilayer membranes.

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Section: Rheometrymentioning

confidence: 99%

Section: Structural Transition Mechanismmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Rheological characterization of thermal phase behavior of anionic lipid DMPG dispersions

Fujii

2017

J Biorheol

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“…Dimyristoylphosphatidylglycerol (DMPG) bilayers have been extensively studied as a model for the negatively charged biomembrane (Eklund et al, 1987;Lotta et al, 1988;Kodama et al, 1993;Zhang et al, 1997;Garidel et al, 2000;Toca-Herrera et al, 2000;Riske et al, 2001;Lamy-Freund and Riske, 2003;Lewis et al, 2005). Recently, it has been reported that DMPG bilayers show a unique low-temperature phase behavior depending on environmental conditions.…”

Section: Introductionmentioning

confidence: 99%

NaCl-dependent formation of the highly crystalline phase in sufficiently hydrated dimyristoylphosphatidylglycerol bilayers

Kinoshita

Kato

Takahashi

2009

Chemistry and Physics of Lipids

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We investigated the low-temperature phase behavior of dimyristoylphosphatidylglycerol (DMPG) bilayers in the presence of high concentration of NaCl (≥100 mM). Differential scanning calorimetry showed that the highly crystalline (HC) phase grew after an initial delay period when DMPG bilayers were sufficiently hydrated and incubated at 1˚C in the presence of more than 100 mM NaCl. The HC phase formation reached a plateau, the level of which depended on NaCl concentration; all the lipids were unable to be in the HC phase at the plateau stage without a quite high concentration of NaCl. Since electron microscopic observations suggested that the HC phase formed coexists with the precursor phases in a closed vesicle, elastic constrain and/or shortage of free sodium ions in the inside of the closed vesicle may prevent the complete transition into the HC phase.3

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The third and fourth transmembrane domains of Slc11a1: Comparison of their structures and positioning in phospholipid model membranes

Yang

Xue

et al. 2008

Biopolymers

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Interactions of two peptides TM3 and TM4, corresponding to the third and fourth transmembrane domains of a divalent metal-ion transporter Slc11a1, respectively, with phospholipid model membranes, including zwitterionic dimyristoylphosphatidylcholine (DMPC) and anionic dimyristoylphosphatidylglycerol (DMPG), are studied in a wide range of peptide-to-lipid (P:L) ratios, and the secondary structures and positioning of the peptides in the lipid bilayers are analyzed, using differential scanning calorimetry (DSC), circular dichroism (CD), and fluorescence techniques. DSC and fluorescence results indicate that both peptides are inserted in the phospholipid bilayers. TM4 is buried in the bilayers more deeply and the insertion position is less affected by pH values, whereas TM3 is less deeply embedded and the position is remarkably shifted toward the direction of the bilayer surface with increasing pH value. The incorporation of TM4 in both lipids causes more perturbation to the packing of the hydrophobic chains of the lipids than TM3. Moreover, the perturbation of TM3 to the lipid packing is pH dependent, whereas the perturbation of TM4 is less pH dependent but with an unusually larger change in the enthalpy of the main transition at pH 5.5 than those at pH 4 and 7. CD data demonstrate that the main discrimination between the secondary structures of TM4 and TM3 is the alpha-helix content, more helicity for TM4 than TM3 in both lipids. The folding of the peptides is also affected by the composition of the phospholipids. Anionic lipid DMPG induces more alpha-helical folding than neutral lipid DMPC for both peptides.

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The peculiar thermo-structural behavior of the anionic lipid DMPG

Cited by 91 publications

References 40 publications

Rheological characterization of thermal phase behavior of anionic lipid DMPG dispersions

Rheological characterization of thermal phase behavior of anionic lipid DMPG dispersions

NaCl-dependent formation of the highly crystalline phase in sufficiently hydrated dimyristoylphosphatidylglycerol bilayers

The third and fourth transmembrane domains of Slc11a1: Comparison of their structures and positioning in phospholipid model membranes

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