The PdeWRKY65-UGT75L28 gene module negatively regulates lignin biosynthesis in poplar petioles

PdeERF114 recruits PdeWRKY75 to regulate callus formation in poplar by modulating the accumulation of H2O2 and the relaxation of cell walls

“…To investigate whether other genes work together with PdeWRKY75 to regulate callus formation, we used a Y2H assay to screen a cDNA library constructed by using cDNA isolated from multiple tissues, including roots, leaves, and stem bases with calli. This cDNA library was used in our previous studies (Xiao et al, 2020;Zhang et al, 2020Zhang et al, , 2022Nvsvrot et al, 2023). A clone with a cDNA fragment that showed top identity to Potri.012G108500 was identified.…”

Variable lignin structure revealed inPopulusleaves

“…Enzyme lignin was analyzed via 2D HSQC NMR with a Bruker Avance II 500-MHz spectrometer. Approximately 40 mg of lignin enriched residue was dissolved in DMSO-d 6 in a 5 mm NMR tube and subsequently sonicated for 1 h. The standard hsqcetgpsip2.2 Bruker pulse sequence was used with an N2 cryoprobe with the following specications: 1 H spectra width of 12 ppm and 1024 data points; 13 All results are presented on an S + G basis and should be interpreted as abundance per 100 aromatic units. Sample 856-S was selected for a whole cell wall NMR analysis.…”

“…11,12 In one study, PdeWRKY65-UGT75L28 was found to impact lignication of leaf petioles, which could impact the transport of water or nutrients and ultimately plant growth. 13 Several MYB proteins have been implicated in the lignication of stem lignin. Additionally, two MYB proteins have also been shown to impact the lignication of leaves.…”