The Patterns of Labeling of Germinal-Center Cells With Tritiated Deoxycytidine

Changes in 2′-deoxycytidine levels in various tissues of tumor-bearing mice

“…In spleen, the weight, level of dCyd and net amount of dCyd were all significantly increased in tumor-bearing mice, compared to the non-tumor groups, on both day 16 and day 22. Osogoe et al reported that after tritiated dCyd was administered to mice intraperitoneally, radioactivity was strongly detected in the germinal center cells of the spleen and in Peyer's patches of the intestine in both mouse (9) and rat (10). We speculate whether dCyd may act as a defense mechanism in the body, given the spleen's role among the lymphatic tissues and its involvement in the reticuloendothelial system.…”

Effects of Refeeding Subsequent to Starvation on the Plasma Cell Population in the Villous Lamina Propria of the Rat Small Intestine

“…Autoradiographic cellular labeling with [311Jdeoxy-cytidine. To examine the cellular activity, particularly of B cell precursors in the Peyer's patches, autoradiographic cellular labeling with [31-11deoxycy-tidine was performed according to the methods described by Osogoe et al (1973). Autoradiographs were prepared 24 hr after intraperitoneal injection of…”

Incorporation of Pyrimidine Nucleosides in Lymphoid Tissues of the Mouse Using 5-Fluorodeoxyuridine and [5-3H]Deoxycytidine

“…Furthermore, it has been shown that the ratio of radioactivity of thymine to that of cytosine in newly synthesized DNA is different among lymphoid tissues; this value in the thymus being higher than that in mesenteric lymph nodes (2,11,12). Thus, it is probable that the differences in labeling intensity between lymphocyte populations may be related to their different capacity for converting CdR to thymidine monophosphate (dTMP) and then utilizing the dTMP for DNA synthesis (1,2,12,17,18,22). Such a hypothesis has not been adequately tested ; much less have the reasons been explored which might account for the differences in the incorporation of radioactive DNA precursors by different lymphoid cell populations.…”

“…In the thymic cortex and germinal centers of mesenteric lymph nodes, however, radioautographic reactions showed more intense labeling with [G-3H] Although mitotic analyses consistently reveal high rates of cell division in the thymic cortex and germinal centers of rats and mice (1,4,5,9,10), the cells in these areas are characterized by weak labeling with tritiated thymidine ([3H]TdR), whereas lymphocytes outside these anatomical regions of the same organs are labeled intensely with the same nucleoside (1, 6-10, 12, 18, 25). Recently, tritiated deoxycytidine ([3H]CdR) or radioiodine-labeled 5-iodo-2'-deoxyuridine has been used as a substitute for [3H]TdR to study lymphocyte production (1,2,12,14,(16)(17)(18)(19)(20)(21)23). Radioautographs revealed that lymphocytes in the thymic cortex and germinal centers were much more heavily labeled with generally tritiated deoxycytidine ([G-3H]CdR) than with [3H]TdR, whereas outside the germinal centers labeling was far more intense * present address:…”

“…Recently, tritiated deoxycytidine ([3H]CdR) or radioiodine-labeled 5-iodo-2'-deoxyuridine has been used as a substitute for [3H]TdR to study lymphocyte production (1,2,12,14,(16)(17)(18)(19)(20)(21)23). Radioautographs revealed that lymphocytes in the thymic cortex and germinal centers were much more heavily labeled with generally tritiated deoxycytidine ([G-3H]CdR) than with [3H]TdR, whereas outside the germinal centers labeling was far more intense with [3H]TdR than with [G-3H]CdR (1,12,18). When rats were given nonradioactive CdR together with [3H]TdR, the weak labeling of cells in the thymic cortex and germinal centers decreased even further, whereas there was only slight reduction in the labeling intensity of cells in the thymic medulla and outside the germinal centers (1).…”