The Pattern of Supernumerary Macrochaetae in Certain Drosophila Mutants

Neel, James V.

doi:10.1093/genetics/25.3.251

Cited by 11 publications

(1 citation statement)

References 11 publications

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“…The presence of a single ZO family member in Drosophila, Pyd, facilitates analysis of its roles. Pyd was identified via alleles that lead to supernumerary adult bristles (Neel, 1940; Chen et al ., 1996); mutants also have adult eye and embryonic tracheal defects (Jung et al ., 2006). The gene is complex with 11 potential isoforms and 3 predicted translation start sites, and these original alleles were not null but reduced or altered expression in tissue specific ways.…”

Section: Introductionmentioning

confidence: 99%

Polychaetoid/ZO-1 strengthens cell junctions under tension while localizing differently than core adherens junction proteins

Schmidt

Finegan

Häring

et al. 2023

Preprint

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During embryonic development dramatic cell shape changes and movements re-shape the embryonic body plan. These require robust but dynamic linkage between the cell-cell adherens junctions and the force-generating actomyosin cytoskeleton. Our view of this linkage has evolved, and we now realize linkage is mediated by a mechanosensitive multiprotein complex assembled via multivalent connections. Here we combine genetic, cell biological and modeling approaches to define the mechanism of action and functions of an important player,DrosophilaPolychaetoid, homolog of mammalian ZO-1. Our data reveal that Pyd reinforces cell junctions under elevated tension, and facilitates cell rearrangements. Pyd is important to maintain junctional contractility and in its absence cell rearrangements stall. We next use structured illumination microscopy to define the molecular architecture of cell-cell junctions during these events. The cadherin-catenin complex and Cno both localize to puncta along the junctional membrane, but are differentially enriched in different puncta. Pyd, in contrast, exhibits a distinct localization to strands that extend out from the region occupied by core junction proteins. We then discuss the implications for the protein network at the junction-cytoskeletal interface, suggesting different proteins localize and function in distinct ways but combine to produce robust connections.

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Section: Introductionmentioning

confidence: 99%

Polychaetoid/ZO-1 strengthens cell junctions under tension while localizing differently than core adherens junction proteins

Schmidt

Finegan

Häring

et al. 2023

Preprint

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Phenocopies inDrosophila hydei induced by actinomycin D and fluorouracil with special reference toNotch mutants

Breugel

Vermet-Rozeboom

Gloor

1975

Wilhelm Roux' Archiv

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Injection of actinomycin D (AMD) and fluorouracil (FU) into late third instar larvae brought about a fairly constant spectrum of phenodeviations in the imago of various genotypes ofDrosophila hydei including wild type. Common phenes were eye facet disarray, fusion of tarsal segments and to a lesser extent also eye reduction. AMD-reduced eyes ofwhite-mottled mutants also showed darker pigmentation than untreated controls.Notch mutants were more sensitive to AMD than non-Notch animals, as regards leg and bristle phenocopies.The most obvious difference between the two substances was their differential effect on bristle formation. AMD caused supernumerary bristles of thesplit mutant type, whereas FU tended to suppress bristle formation. The spectrum of malformations, especially after AMD treatment, resembled the temperature-sensitive phenes of certainNotch alleles ofD. melanogaster and suggests that AMD interferes with the transcription of the complexNotch gene.

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Arrangement of bristles as a function of bristle number on a leg segment inDrosophila melanogaster

Held

1990

Roux's Arch Dev Biol

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The arrangement of bristles on a leg segment of the fruitflyDrosophila melanogaster was studied in various mutants that have abnormal numbers of bristles on this segment. Eighteen mutations at six different genetic loci were analyzed, plus five double or triple mutant combinations. Recessive mutations at theachaete-scute locus were found to affect distinct groups of bristles:achaete mutations remove mechanosensory bristles, whereasscute mutations remove mainly chemosensory bristles. Mechanosensory bristles remain uniformly spaced along the longitudinal axis unless their number decreases below a certain threshold, suggesting that spacing is controlled by cell interactions that cannot function when bristle cells are too far apart. Above a certain threshold, bristle spacing and alignment both become irregular, perhaps due to excessive force from these same interactions. Chemosensory bristles occupy definite positions that are virtually unaffected by removal of individual bristles from the array. Extra chemosensory bristles develop only near the six normal sites. At two of the six sites the multiple bristles tend to exhibit uniform longitudinal spacing - a property confined to mechanosensory bristles in wild-type flies. To explain the various mutant phenotypes the following scheme is proposed, with different mutations directly or indirectly affecting each step: (1) spots and stripes are demarcated within the pattern area, (2) one bristle cell normally arises within each spot, multiple bristle cells within each stripe, (3) incipient bristle cells inhibit neighboring cells from becoming bristle cells, and (4) the bristle cells within each stripe become aligned to form rows and then repel one another to generate uniform spacing.

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The Pattern of Supernumerary Macrochaetae in Certain Drosophila Mutants

Cited by 11 publications

References 11 publications

Polychaetoid/ZO-1 strengthens cell junctions under tension while localizing differently than core adherens junction proteins

Polychaetoid/ZO-1 strengthens cell junctions under tension while localizing differently than core adherens junction proteins

Phenocopies inDrosophila hydei induced by actinomycin D and fluorouracil with special reference toNotch mutants

Arrangement of bristles as a function of bristle number on a leg segment inDrosophila melanogaster

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