The pattern of cervical penetration and the effect of topical treatment with prostaglandin and/or FSH and oxytocin on the depth of cervical penetration in the ewe during the peri-ovulatory period

Falchi, Laura; Taema, Maged M.; Clanche, S. La; Scaramuzzi, R. J.

doi:10.1016/j.theriogenology.2012.02.017

Cited by 20 publications

(22 citation statements)

References 32 publications

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“…Group OE presented the highest cervical penetration rate and this protocol was previously described with success in other studies aiming for oocyte recovery (Flohr et al, 1999) or embryo transfer ) in sheep. Flohr et al (1999 used the same doses, times of drugs administration and routes used in our study.…”

Section: Discussionmentioning

confidence: 84%

Cervical relaxation for non-surgical uterus access in Santa Inês ewes

Leite

Fonseca

Fernandes

et al. 2018

Arq. Bras. Med. Vet. Zootec.

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The present study was composed by two experiments aiming to develop a cervical dilation technique for non-surgical access to Santa Inês ewe's uterus. In Experiment 1, thirty ewes underwent four epidural treatments. The three experimental treatments used 2.0mg/kg ketamine. The group receiving this drug alone was denominated KG, whereas other group had ketamine associated with 0.1mg/kg morphine (KM) and KX a third group had ketamine associated with 0.05mg/kg xylazine (KX). Control treatment was 1mL/7.5kg saline solution epidurally (CON). Cervical dilation was evaluated in both experiments by attempting to pass a metal rod through the cervix. In Experiment 2, three different hormonal protocols for cervical dilation were tested in thirty ewes. Epidural anesthesia with 2.0mg/kg ketamine was the control treatment (KG) and was combined with hormonal treatments: Misoprostol (MI); Oxytocin + Estradiol (OE); Misoprostol + Oxytocin + Estradiol (MOE). In Experiment 1 transposition rate was not different among groups. In Experiment 2, OE presented the highest rate (90%) while MOE presented 86.2%, MI 68.9% and CON 62.1%. The study developed a pharmacological protocol that increased cervical transposition making the non-surgical access to the uterus feasible in Santa Inês ewes. RESUMOO presente estudo teve como objetivo desenvolver uma técnica para acesso não cirúrgico ao útero de ovelhas Santa Inês e foi realizado em duas etapas. No experimento 1, 30 ovelhas foram submetidas a quatro tratamentos epidurais. Os três tratamentos teste usaram 2,0mg/kg de cetamina. O grupo que recebeu apenas cetamina foi denominado KG, enquanto no segundo grupo a cetamina foi combinada com morfina (0,1mg/kg -KM) e um terceiro grupo recebeu cetamina associada com xilazina 0,05mg/kg -KX). No grupo controle (CON), usou-se solução salina (1mL/7,5kg de peso). A dilatação cervical foi avaliada em ambos os experimentos pela tentativa de transposição cervical com uma haste metálica. No experimento 2, três protocolos hormonais de dilatação cervical foram testados em 30 ovelhas. Anestesia epidural com 2,0mg/kg de cetamina foi o tratamento controle (CON), combinada com tratamentos hormonais nos grupos: misoprostol (MI); ocitocina + estradiol (OE); misoprostol + ocitocina + estradiol (MOE). No experimento 1, a taxa de transposição não variou entre os grupos. No experimento 2, OE teve a maior taxa (90%), enquanto MOE apresentou 86,2%, MI i 68,9% e CON 62,1%. O estudo desenvolveu um protocolo farmacológico que aumentou a taxa de transposição cervical, tornando o acesso uterino não cirúrgico viável em ovelhas Santa Inês.

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Section: Discussionmentioning

confidence: 84%

Cervical relaxation for non-surgical uterus access in Santa Inês ewes

Leite

Fonseca

Fernandes

et al. 2018

Arq. Bras. Med. Vet. Zootec.

View full text Add to dashboard Cite

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“…However, for the moment, this remains an intractable problem (Falchi et al. ), and it remains an important objective of research for the sheep breeding industry. The tissues implicated in the softening of the cervical canal are the subepithelial stroma and smooth muscle (Kershaw et al.…”

Section: Discussionmentioning

confidence: 99%

The Effect of the Intracervical Application of Follicle‐Stimulating Hormone or Luteinizing Hormone on the Pattern of Expression of Gonadotrophin Receptors in the Cervix of Non‐Pregnant Ewes

Leethongdee

Khalid

Scaramuzzi

2014

Reprod Domestic Animals

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This is the peer reviewed version of an accepted journal article -'The effect of the intracervical application of Follicle Stimulating Hormone (FSH) or Luteinizing Hormone (LH) on the pattern of expression of gonadotrophin receptors in the cervix of nonpregnant ewes' -which has been published in final form at https://dx.doi.org/10.1111/rda.12312. This draft has been made available on the RVC's Open Access publications repository in accordance with the College's Open Access policy. This article may be used for non-commercial purposes in accordance with the publisher's self-archiving policy, which can be found at http://olabout.wiley.com/WileyCDA/Section/id-820227.html. Control. Cervices were collected 54h after sponge removal and then divided into 3 regions. 26The expression of FSHR and LHR was determined by immunohistochemistry and FSHR

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“…It has been shown that the levels of some of the potential mediators in the pathway of PG synthesis are related to the pattern of cervical relaxation in sheep. In particular, the levels of estradiol receptor a, OTR, cPLA 2 , and COX-2 were all elevated during the periovulatory period [6,18] when cervical penetrability was the greatest [3,4]. We suggested that the high circulating concentrations of FSH and estradiol at this time of the estrous cycle stimulated cervical production of PGE 2 probably at multiple levels.…”

Section: Discussionmentioning

confidence: 79%

“…Previous studies from our research group have reported that there is a degree of relaxation and dilation of the cervix during estrus [3][4][5]. Cervical relaxation is due in part, to the rearrangement of collagen bundles and fibers that disperse and "soften" the extracellular matrix reducing the tensile strength of the cervix [6] thus allowing the smooth muscle to dilate the cervical canal.…”

Section: Discussionmentioning

confidence: 96%

“…It has been suggested that the gonadotrophic hormones and estradiol that are present in higher concentrations at this time might have a role in this mechanism [3][4][5]. Thus, understanding the physiology of the cervix and the mechanisms that mediate its relaxation during estrus might inform the on-going attempts to develop effective methods of transcervical intrauterine insemination in ewes.…”

Section: Introductionmentioning

confidence: 99%

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