A técnica de gradiente difusivo de membrana (DGT) foi utilizada para determinar metais lábeis in situ durante diferentes condições hidrológicas no estuário da Lagoa dos Patos. As amostras de água foram também analisadas para a fração lábil-Chelex para Cd, Co, Cu, Mn, Ni e Zn usando resina Chelex-100. As concentrações lábeis-DGT de Cd, Cu, Ni e Zn foram mais baixas que a lábil-Chelex, possivelmente devido à ligação do metal coloidal na coluna de resina Chelex. Os resultados do DGT indicaram um decréscimo nas concentrações de todos os elementos, refletindo trocas na especiação dos metais em solução causada pela diminuição da salinidade e associado a mudanças de pH no estuário. Os resultados demonstraram a sensibilidade do DGT para a especiação dos metais e a capacidade do uso da técnica DGT em águas estuarinas altamente dinâmicas para a obtenção de concentrações de metais lábeis integradas ao longo do tempo.The diffusive gradients in thin-films (DGT) technique was used to determine labile metals in situ during different hydrological conditions in the Patos Lagoon estuary. Water samples were also analyzed for Chelex-labile fractions of Cd, Co, Cu, Mn, Ni and Zn using Chelex-100 resin. DGT-labile concentrations of Cd, Cu, Ni and Zn were lower than Chelex-labile concentrations, possibly because colloids were trapped in the Chelex resin column. There was a decrease with increasing deployment time in the concentrations of all elements measured by DGT, reflecting changes in metal speciation in solution caused by the decrease in the salinity and associated with change in pH in the estuary. The results demonstrated the sensitivity of DGT to metal speciation and the feasibility of using the DGT technique in highly dynamic estuarine waters to obtain a time-integrated record of labile trace metals.
Keywords: DGT, labile metal, estuary
IntroductionTrace metals are one of the major contaminants of estuarine and coastal areas, as a result of coastal development and, consequently, inputs from many anthropogenic sources. 1 Elements such as Cu, Ni, Mn and Zn play an important role as essential elements in aquatic systems. However, elevated concentrations of these elements, and of nonessential elements such as Cd, may cause toxicity for aquatic organisms. The investigation of metal bioavailability in water is as important as metal concentration when considering the impact of metals on an environment.The free metal ion (M 2+ ) and labile metal species in solution are the forms of dissolved metal often accumulated by aquatic organisms. 2 Uptake may occur directly into phytoplankton cells 3 or across gill membranes in fish. 4 So, speciation studies of trace metals have become important, as it enables evaluation of trace metal bioavailability and mobility. Available techniques include anodic stripping voltammetry, 5 cathodic stripping voltammetry 6 and ionexchange columns. 7 However, with standard equipment, all these methods require analyses to be done on a sample brought to the laboratory and metal speciation may change in this time ...