The pathway for perception and transduction of low-temperature signals in Synechocystis

Suzuki, Iwane; Los, Dmitry A.; Kanesaki, Yu; Mikami, Koji; Murata, Norio

doi:10.1093/emboj/19.6.1327

Cited by 249 publications

(191 citation statements)

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“…Each of the putative hik genes in Synechocystis was inactivated previously by insertion of a spectinomycin-resistance gene cassette to create a gene knockout library of DHik cells (Suzuki et al, 2000; CyanoMutants, http://www.kazusa.or.jp/ cyano/Synechocystis/mutants). We examined the genome-wide expression of genes in wild-type cells and in all the lines of Dhik mutant cells using DNA microarrays that covered 3,075 of the 3,267 genes in the Synechocystis chromosome in an attempt to identify Hiks that might be involved in the regulation of expression of heat shock genes ( Fig.…”

Section: Screening Of An Hik Knockout Library Revealed That Mutation mentioning

confidence: 99%

“…Construction of a mutant Hik library has been described elsewhere (Suzuki et al, 2000; http://www.kazusa.or.jp/cyanobase/Synechocystis/mutants).…”

Section: Construction Of Hik34 Mutant Cells and Cells Thatmentioning

confidence: 99%

“…The plasmid with the spectinomycin-resistance gene cassette within the cloned hik34 gene fragment was used to transform Synechocystis cells (Williams, 1988). Spectinomycin-resistant colonies were isolated and cells with a fully segregated mutation were obtained after growth for several generations in BG-11 media that contained the antibiotic, as previously reported (Suzuki, et al, 2000Yamaguchi et al, 2002).…”

Section: Construction Of Hik34 Mutant Cells and Cells Thatmentioning

confidence: 99%

“…Cells were harvested from the mixture by centrifugation at 4,000g, for 5 min at 4°C, and then frozen immediately and stored at 280°C. Total RNA was extracted with hot phenol as described previously (Suzuki et al, 2000). A Synechocystis DNA microarray (CyanoCHIP) was purchased from TaKaRa Bio Co. Ltd. (Shiga, Japan).…”

Section: Dna Microarray Analysismentioning

confidence: 99%

“…The chromosome of Synechocystis includes 44 putative genes for Hiks that are candidates for sensors of environmental signals Mizuno et al, 1996). It has already been demonstrated that the Hiks in Synechocystis are involved in the perception and transduction of signals due to cold stress (Suzuki et al, 2000, phosphate deprivation (Hirani et al, 2001;Suzuki et al, 2004), high osmolarity Paithoonrangsarid et al, 2004), heavy metal ions (Ló pez-Maury et al, 2002;Ogawa et al, 2002;Yamaguchi et al, 2002), and high concentrations of NaCl (Marin et al, 2003). In this article, we provide evidence that Hik34, one of the 44 putative Hiks in Synechocystis, is involved in the control of the expression of heat shock genes and the regulation of thermotolerance in Synechocystis.…”

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confidence: 99%

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The Histidine Kinase Hik34 Is Involved in Thermotolerance by Regulating the Expression of Heat Shock Genes in Synechocystis

et al. 2005

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Histidine kinases (Hiks) in Synechocystis sp. PCC 6803 are involved in the transduction of signals associated with various kinds of environmental stress. To examine the potential role in thermotolerance of Hiks, we used genome microarray analysis to screen a Hik knockout library for mutations that affected the expression of genes for heat shock proteins. Mutation of the hik34 gene enhanced the levels of transcripts of a number of heat shock genes, including htpG and groESL1. Overexpression of the hik34 gene repressed the expression of these heat shock genes. In addition, the cells with a mutant gene for Hik34 (DHik34 cells) survived incubation at 48°C for 3 h, while wild-type cells and cells with mutations in other Hiks were killed. However, mutation of the hik34 gene had only an insignificant effect on the global expression of genes upon incubation of the mutant cells at 44°C for 20 min. Quantitative two-dimensional gel electrophoresis revealed that levels of GroES and HspA were elevated in DHik34 cells after incubation of cells at 42°C for 60 min. We overexpressed recombinant Hik34 protein in Escherichia coli and purified it. We found that the protein was autophosphorylated in vitro at physiological temperatures, but not at elevated temperatures, such as 44°C. These results suggest that Hik34 might negatively regulate the expression of certain heat shock genes that might be related to thermotolerance in Synechocystis.

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Section: Screening Of An Hik Knockout Library Revealed That Mutation mentioning

confidence: 99%

“…Construction of a mutant Hik library has been described elsewhere (Suzuki et al, 2000; http://www.kazusa.or.jp/cyanobase/Synechocystis/mutants).…”

Section: Construction Of Hik34 Mutant Cells and Cells Thatmentioning

confidence: 99%

Section: Construction Of Hik34 Mutant Cells and Cells Thatmentioning

confidence: 99%

Section: Dna Microarray Analysismentioning

confidence: 99%

mentioning

confidence: 99%

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The Histidine Kinase Hik34 Is Involved in Thermotolerance by Regulating the Expression of Heat Shock Genes in Synechocystis

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Molecular Bases of Plant Adaptation to Abiotic Stress and Approaches to Enhance Tolerance to Hostile Environments

Coraggio

Tuberosa

2004

Handbook of Plant Biotechnology

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Abiotic stress has become the greatest threat to global food production and security, particularly in developing countries. Progress in increasing yield and its stability under adverse conditions through a direct selection has been hampered by the low heritability of yield, especially under drought. A better knowledge of the molecular and genetic basis of the mechanisms promoting tolerance to abiotic stress will enhance our capacity to more appropriately manipulate the genes limiting crop yield under hostile environments. This review describes and critically analyses the major advances in our understanding of the complex mechanisms underlying the adaptive response of the plant to abiotic stress, starting with the perception of the stress at the cellular level, its transduction through a cascade of signalling molecules and ending with the induction of genes whose products mitigate the harmful effects of the stress. Molecular markers and the ‘omics’ platforms (e.g., transcriptomics, proteomics, metabolomics etc.) offer unprecedented opportunities to identify and clone the genes governing plant adaptation to environmental stress and limiting yield under such conditions. The dissection of the genetic basis of quantitative traits into their single components, the so‐called QTLs (quantitative trait loci), allows us to deploy marker‐assisted selection (MAS) for enhancing crop performance under adverse conditions. Additional opportunities are also offered by genetic engineering, particularly through the expression of transcription factors regulating a suite of genes whose product is crucial to mitigate the effects of stress. Although the applicative results so far achieved with non‐conventional approaches have been limited, their integration with conventional breeding methodologies and other interdisciplinary approaches will greatly enhance our capacity to more effectively tailor crop genome to withstand harsh environmental conditions and to improve their sustainability under marginal conditions.

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Plant Abiotic Stress

Jenks

Hasegawa

2005

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of CBF gene expression in response to low temperature 4.2.4.1 DNA regulatory elements controlling CBF expression 4.2.4.2 Proteins with positive roles in CBF expression 4.2.4.3 Proteins with negative roles in CBF expression 4.2.4.4 Other potential CBF regulatory proteins 4.2.4.5 Light and circadian rhythms 4.2.4.6 Role of calcium 4.2.4.7 Role of ABA 4.3 Conservation of the CBF cold-response pathway 4.3.1 Brassica napus 4.3.2 Tomato 4.3.3 Rice 4.4 Concluding remarks 5 Plant responses to high temperature JANE LARKINDALE, MICHAEL MISHKIND and ELIZABETH VIERLING 5.1 Introduction 5.2 Physiological responses to high temperature 5.2.1 High temperature limits to optimal plant performance 5.2.2 Heat sensitivity of photosynthesis 5.2.3 Heat sensitivity of reproduction 5.3 Cellular acquired thermotolerance 5.4 Heat shock proteins/molecular chaperones 5.4.1 Hsp100/ClpB 5.4.2 Hsp90 5.4.3 Hsp70/DnaK 5.4.4 Hsp60/GroE 5.4.5 The sHSP family of proteins 5.5 Other components of the response to heat 5.5.1 Antioxidant production 5.5.2 Other heat-stress regulated genes 5.5.3 Other heat-protective responses 5.5.4 Mutants defective in heat tolerance 5.5.5 Transgenic plants with altered heat tolerance CONTENTS vii 5.6 Signaling pathways involved in response to heat 5.6.1 Heat shock transcription factors 5.6.2 Other signaling pathways 5.6.3 Abscisic acid 5.6.4 Salicylic acid

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The pathway for perception and transduction of low-temperature signals in Synechocystis

Cited by 249 publications

References 42 publications

The Histidine Kinase Hik34 Is Involved in Thermotolerance by Regulating the Expression of Heat Shock Genes in Synechocystis

The Histidine Kinase Hik34 Is Involved in Thermotolerance by Regulating the Expression of Heat Shock Genes in Synechocystis

Molecular Bases of Plant Adaptation to Abiotic Stress and Approaches to Enhance Tolerance to Hostile Environments

Plant Abiotic Stress

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