The pathological response and fate in the lung and pleura of chrysotile in combination with fine particles compared to amosite asbestos following short-term inhalation exposure: interim results

Bernstein, David M.; Rogers, Rick A.; Sepulveda, R.; Donaldson, Ken; Schuler, Detlef; Gaering, S.; Kunzendorf, P.; Chevalier, J.; Holm, Stewart E.

doi:10.3109/08958378.2010.497818

Cited by 36 publications

(39 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The results from the histopathological examination of the lungs have been presented in detail in Bernstein et al (2010). These findings confirmed that animals exposed to CPP produced no sign of pulmonary inflammation, aside from a macrophage response, at any time point.…”

Section: Resultssupporting

confidence: 61%

“…The bivariate length and diameter size distributions of the CSP aerosol and the amosite asbestos aerosol have been presented in Bernstein et al (2010).…”

Section: Resultsmentioning

confidence: 99%

“…The methods for the gravimetric determination of aerosol concentrations; sampling of fiber number and size distribution of aerosol concentrations; particle size of dust aerosol; counting rules for the evaluation of aerosol and lung burden samples by TEM; and clinical examination and body weights have been presented in Bernstein et al (2008 and 2010). …”

Section: Methodsmentioning

confidence: 99%

“…In addition, the pathological response was quantified by compartment in the lung and translocation of fibers to and pathological response in the pleural cavity determined. The interim results from the study were presented in Bernstein et al (2010). This paper presents the final results from the study, including the details of the measurements in the pleural cavity.…”

Section: Introductionmentioning

confidence: 99%

“…The specification and preparation of the Ready-mix used to produce the sanded material has been described previously (Brorby et al, 2008; Bernstein et al, 2010). Extensive characterization including comparison of the bivariate size distribution was performed to confirm that the chrysotile used in the recreated formulation in this study (JM 7RF3 from the leffrey Mine in Quebec, Canada) closely matched that from a historical sample of the joint compound (Brorby et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Quantification of the pathological response and fate in the lung and pleura of chrysotile in combination with fine particles compared to amosite-asbestos following short-term inhalation exposure

Bernstein

Rogers

Sepulveda

et al. 2011

Inhalation Toxicology

Self Cite

View full text Add to dashboard Cite

The marked difference in biopersistence and pathological response between chrysotile and amphibole asbestos has been well documented. This study is unique in that it has examined a commercial chrysotile product that was used as a joint compound. The pathological response was quantified in the lung and translocation of fibers to and pathological response in the pleural cavity determined. This paper presents the final results from the study. Rats were exposed by inhalation 6 h/day for 5 days to a well-defined fiber aerosol. Subgroups were examined through 1 year. The translocation to and pathological response in the pleura was examined by scanning electron microscopy and confocal microscopy (CM) using noninvasive methods.The number and size of fibers was quantified using transmission electron microscopy and CM. This is the first study to use such techniques to characterize fiber translocation to and the response of the pleural cavity. Amosite fibers were found to remain partly or fully imbedded in the interstitial space through 1 year and quickly produced granulomas (0 days) and interstitial fibrosis (28 days). Amosite fibers were observed penetrating the visceral pleural wall and were found on the parietal pleural within 7 days postexposure with a concomitant inflammatory response seen by 14 days. Pleural fibrin deposition, fibrosis, and adhesions were observed, similar to that reported in humans in response to amphibole asbestos. No cellular or inflammatory response was observed in the lung or the pleural cavity in response to the chrysotile and sanded particles (CSP) exposure. These results provide confirmation of the important differences between CSP and amphibole asbestos.

show abstract

Section: Resultssupporting

confidence: 61%

“…The bivariate length and diameter size distributions of the CSP aerosol and the amosite asbestos aerosol have been presented in Bernstein et al (2010).…”

Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Quantification of the pathological response and fate in the lung and pleura of chrysotile in combination with fine particles compared to amosite-asbestos following short-term inhalation exposure

Bernstein

Rogers

Sepulveda

et al. 2011

Inhalation Toxicology

Self Cite

View full text Add to dashboard Cite

show abstract

Mesothelioma and Analysis of Tissue Fiber Content

Neumann

Löseke

Tannapfel

2011

Malignant Mesothelioma

View full text Add to dashboard Cite

The strong relationship between mesothelioma and asbestos exposure is well established. The analysis of lung asbestos burden by light and electron microscopy assisted to understand the increased incidence of mesothelioma in asbestos mining and consuming nations.The data on the occupational exposure to asbestos are important information for the purpose of compensation of occupational disease No. 4105 (asbestos-associated mesothelioma) in Germany.However, in many cases the patients have forgotten conditions of asbestos exposure or had no knowledge about the used materials with components of asbestos. Mineral fiber analysis can provide valuable information for the research of asbestos-associated diseases and for the assessment of exposure. Because of the variability of asbestos exposure and long latency periods, the analysis of asbestos lung content is a relevant method for identification of asbestos-associated diseases. Also, sources of secondary exposure, so called "bystander exposition" or environmental exposure can be examined by mineral fiber analysis.Household contacts to asbestos are known for ten patients (1987-2009) in the German mesothelioma register; these patients lived together with family members working in the asbestos manufacturing industry.Analysis of lung tissue for asbestos burden offers information on the past exposure. The predominant fiber-type identified by electron microscopy in patients with mesothelioma is amphibole asbestos (crocidolite or amosite). Latency times (mean 42.5 years) and mean age at the time of diagnose in patients with mesothelioma are increasing (65.5 years). The decrease of median asbestos burden of the lung in mesothelioma patients results in disease manifestation at a higher age.Lung dust analyses are a relevant method for the determination of causation in mesothelioma. Analysis of asbestos burden of the lung and of fiber type provides insights into the pathogenesis of malignant mesothelioma. The most important causal factor for the development of mesothelioma is still asbestos exposure.

show abstract

Length-Dependent Retention of Carbon Nanotubes in the Pleural Space of Mice Initiates Sustained Inflammation and Progressive Fibrosis on the Parietal Pleura

Murphy

Poland

Duffin

et al. 2011

The American Journal of Pathology

286

263

View full text Add to dashboard Cite

The fibrous shape of carbon nanotubes (CNTs) raises concern that they may pose an asbestos-like inhalation hazard, leading to the development of diseases, especially mesothelioma. Direct instillation of long and short CNTs into the pleural cavity, the site of mesothelioma development, produced asbestos-like length-dependent responses. The response to long CNTs and long asbestos was characterized by acute inflammation, leading to progressive fibrosis on the parietal pleura, where stomata of strictly defined size limit the egress of long, but not short, fibers. This was confirmed by demonstrating clearance of short, but not long, CNT and nickel nanowires and by visualizing the migration of short CNTs from the pleural space by single-photon emission computed tomographic imaging. Our data confirm the hypothesis that, although a proportion of all deposited particles passes through the pleura, the pathogenicity of long CNTs and other fibers arises as a result of length-dependent retention at the stomata on the parietal pleura.

show abstract

The pathological response and fate in the lung and pleura of chrysotile in combination with fine particles compared to amosite asbestos following short-term inhalation exposure: interim results

Abstract: (2010) The pathological response and fate in the lung and pleura of chrysotile in combination with fine particles compared to amosite asbestos following short-term inhalation exposure: interim results, Inhalation Toxicology, 22:11,

Cited by 36 publications

References 21 publications

Quantification of the pathological response and fate in the lung and pleura of chrysotile in combination with fine particles compared to amosite-asbestos following short-term inhalation exposure

Quantification of the pathological response and fate in the lung and pleura of chrysotile in combination with fine particles compared to amosite-asbestos following short-term inhalation exposure

Mesothelioma and Analysis of Tissue Fiber Content

Length-Dependent Retention of Carbon Nanotubes in the Pleural Space of Mice Initiates Sustained Inflammation and Progressive Fibrosis on the Parietal Pleura

Contact Info

Product

Resources

About