The Pathogenicity and Host Immune Response Associated with H5N1 Highly Pathogenic Avian Influenza Virus in Quail

Uno, Yukiko; Usui, Tatsufumi; Soda, Kosuke; Fujimoto, Yoshikazu; Takeuchi, Takashi; Ito, Hiroshi; Ito, Toshihiro; Yamaguchi, Tsuyoshi

doi:10.1292/jvms.12-0433

Cited by 12 publications

(10 citation statements)

References 24 publications

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“…Gene expressions of inter‐leukin 6 (IL‐6), toll‐like receptor (TLR) 7 and interferon (IFN) α in each avian species and retinoic acid inducible (RIG‐I) in Muscovy ducks were assessed by qRT‐PCR. Primer sequences are described in (Cornelissen, Post, Peeters, Vervelde, & Rebel, ; Uno et al., ). Primers were diluted at 2.5 m m following manufacturer instructions.…”

Section: Methodsmentioning

confidence: 99%

Transmission and immunopathology of the avian influenza virus A/Anhui/1/2013 (H7N9) human isolate in three commonly commercialized avian species

Vidaña

Dolz

Busquets

et al. 2017

Zoonoses and Public Health

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H7N9 virus infection is a global concern, given that it can cause severe infection and mortality in humans. However, the understanding of H7N9 epidemiology, animal reservoir species and zoonotic risk remains limited. This work evaluates the pathogenicity, transmissibility and local innate immune response of three avian species harbouring different respiratory distribution of α2,6 and α2,3 SA receptors. Muscovy ducks, European quails and SPF chickens were intranasally inoculated with 10 embryo infectious dose (EID) of the human H7N9 (A/Anhui/1/2013) influenza isolate. None of the avian species showed clinical signs or macroscopic lesions, and only mild microscopic lesions were observed in the upper respiratory tract of quail and chickens. Quail presented more severe histopathologic lesions and avian influenza virus (AIV) positivity by immunohistochemistry (IHC), which correlated with higher IL-6 responses. In contrast, Muscovy ducks were resistant to disease and presented higher IFNα and TLR7 response. In all species, viral shedding was higher in the respiratory than in the digestive tract. Higher viral shedding was observed in quail, followed by chicken and ducks, which presented similar viral titres. Efficient transmission was observed in all contact quail and half of the Muscovy ducks, while no transmission was observed between chicken. All avian species showed viral shedding in drinking water throughout infection.

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Section: Methodsmentioning

confidence: 99%

Transmission and immunopathology of the avian influenza virus A/Anhui/1/2013 (H7N9) human isolate in three commonly commercialized avian species

Vidaña

Dolz

Busquets

et al. 2017

Zoonoses and Public Health

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“…A recent Cochrane review reported that antiviral therapies had little benefit for severe influenza patients [14, 15]. Furthermore, AI continues to require prolonged mechanical ventilation in the intensive care unit, and AI-associated mortality remains high at 30–50 % despite optimal supportive care [3, 16]. …”

Section: Introductionmentioning

confidence: 99%

Mesenchymal stromal cell treatment prevents H9N2 avian influenza virus-induced acute lung injury in mice

et al. 2016

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BackgroundThe avian influenza virus (AIV) can cross species barriers and expand its host range from birds to mammals, even humans. Avian influenza is characterized by pronounced activation of the proinflammatory cytokine cascade, which perpetuates the inflammatory response, leading to persistent systemic inflammatory response syndrome and pulmonary infection in animals and humans. There are currently no specific treatment strategies for avian influenza.MethodsWe hypothesized that mesenchymal stromal cells (MSCs) would have beneficial effects in the treatment of H9N2 AIV-induced acute lung injury in mice. Six- to 8-week-old C57BL/6 mice were infected intranasally with 1 × 104 MID50 of A/HONG KONG/2108/2003 [H9N2 (HK)] H9N2 virus to induce acute lung injury. After 30 min, syngeneic MSCs were delivered through the caudal vein. Three days after infection, we measured the survival rate, lung weight, arterial blood gas, and cytokines in both bronchoalveolar lavage fluid (BALF) and serum, and assessed pathological changes to the lungs.ResultsMSC administration significantly palliated H9N2 AIV-induced pulmonary inflammation by reducing chemokines and proinflammatory cytokines levels, as well as reducing inflammatory cell recruit into the lungs. Thus, H9N2 AIV-induced lung injury was markedly alleviated in mice treated with MSCs. Lung histopathology and arterial blood gas analysis were improved in mice with H9N2 AIV-induced lung injury following MSC treatment.ConclusionsMSC treatment significantly reduces H9N2 AIV-induced acute lung injury in mice and is associated with reduced pulmonary inflammation. These results indicate a potential role for MSC therapy in the treatment of clinical avian influenza.

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“…The important pro-inflammatory cytokines IL-6 and TNF-α cause severe damage to the lungs during influenza infection [28] , and the overexpression of pro-inflammatory cytokines increases the pathogenicity of influenza virus [9,29] . Compared with the model group, the expression of IL-1α, IL-6, and TNF-α in lung tissue was decreased significantly after treatment with GGD and OP.…”

Section: Ggd Regulated the Expression Of Il-1α Il-6 And Tnf-αmentioning

confidence: 99%

Exploration of the mechanisms of Ge Gen Decoction against influenza A virus infection

Geng

Cui

et al. 2019

Chinese Journal of Natural Medicines

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Ge Gen Decoction (GGD), a Traditional Chinese Medicine prescription, is mainly used to treat infectious respiratory diseases and can relieve the symptoms of influenza A virus (IAV) infection. However, the underlying mechanism of GGD against IAV infection remains unclear. In this study, we found that GGD had moderate anti-IAV activity in vitro. GGD was more effective when given before the viral infection and targeted the viral attachment and replication stages rather than the internalization stage. In vivo, GGD treatment reduced the virus titers of lung tissue significantly and improved the survival rate, lung index, and pulmonary histopathological changes in H1N1-infected mice. We observed the changes in several key immuno-related indexes in GGD administrated H1N1-infected mice with anti-IAV drug oseltamivir phosphate as the control. GGD treatment decreased the expression of TNF-α and improved Th1/Th2 immune balance to reduce the excessive immune response in H1N1-infected mice. Besides, the expression of the toll-like receptor 7 signaling pathway in H1N1-infected mice decreased after GGD treatment. Our results showed that GGD has anti-IAV activity and can modulate the immune system to relieve lung inflammation.

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The Pathogenicity and Host Immune Response Associated with H5N1 Highly Pathogenic Avian Influenza Virus in Quail

Cited by 12 publications

References 24 publications

Transmission and immunopathology of the avian influenza virus A/Anhui/1/2013 (H7N9) human isolate in three commonly commercialized avian species

Transmission and immunopathology of the avian influenza virus A/Anhui/1/2013 (H7N9) human isolate in three commonly commercialized avian species

Mesenchymal stromal cell treatment prevents H9N2 avian influenza virus-induced acute lung injury in mice

Exploration of the mechanisms of Ge Gen Decoction against influenza A virus infection

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