The path of carbon in photosynthesis. V. Paper chromatography and radioautography of the products

Benson, A. A.; Bassham, James A.; Calvin, Melvin; Goodale, T.; Haas, Victoria; Stepka, W.

doi:10.2172/4430548

Cited by 98 publications

(124 citation statements)

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“…With AOA, an initial larger percentage of "C incorporation went into these C4 acids before labeling of glycolate, although AOA did not alter the per cent distribution of 14C into these acids as much as into glycolate and alanine. The per cent 14C incorporated into aspartate was significantly increased by AOA from 6.5 to 22.3% after 2 in the cells suspension decreased, presumably from dark respiration (Fig. 4).…”

Section: Resultsmentioning

confidence: 90%

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Aminooxyacetate Stimulation of Glycolate Formation and Excretion by Chlamydomonas

Tolbert

Harrison²,

Selph³

1983

Plant Physiol.

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Section: Resultsmentioning

confidence: 90%

“…Aliquots of 1 to 3 ml of the algal suspension were placed in a photosynthetic vessel consisting of either flattened test tubes or 2 cm diameter glass vials with a flat bottom so that the light path through the solution was about 2 …”

Section: Methodsmentioning

confidence: 99%

Aminooxyacetate Stimulation of Glycolate Formation and Excretion by Chlamydomonas

Tolbert

Harrison²,

Selph³

1983

Plant Physiol.

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“…Coincidence of added carrier citrate and radioactivity was also established by paper electrophoresis at pH 3.6. The chromatography system of Benson et al (1950) was used. In the second procedure, citrate formed as a result of incubation under the conditions of the previous procedure, or alternatively using acetylcoenzyme A (prepared by the method of Stadtman, 1957) in place of the acetic thiokinase system, was measured directly by the colorimetric procedure of Taylor Table 8.…”

Section: Enzyme Activities In Cell-free Extracts Of Anacystis Nidulansmentioning

confidence: 99%

Photoassimilation of organic compounds by autotrophic blue-green algae

Hoare¹,

Moore²

1965

Biochimica et Biophysica Acta (BBA) - Biophysics including Phot

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“…For the manometric determinations, the flasks of spherical plants were emptied over a wire screen or 1 Received December 18,1956. 2 This work was supported, in part, by Research Grant G-1291 from the 'National Science Foundation to the second author.…”

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confidence: 99%

“…The combined extract was concentrated first in a vacuum still and then by blowing nitrogen gas over it. The extracts were examined by the chromatographic and radioautographic methods (1) The effect of the pH of the suspending medium was assessed using phosphate and tartrate buffers.…”

mentioning

confidence: 99%

Respiration of the Mycelia and Mitochondria of the Filamentous Watermold, Allomyces Macrogynus

Bonner

Machlis

1957

Plant Physiol.

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The immediate purpose of this investigation of respiration in the watermold Allomyces macrogynus was to provide a basis for the ultimate explanation of the effect of various nutritional conditions on the utilization of mannose and fructose by this mold (28,29,37). Its primary significance, however, is the isolation from one filamentous fungus of mitochondria which oxidize the various intermediates of the Krebs cycle. Only mitochondria from y-east, among the fungi, have been shown to contain an organized system associated with mitochondria for carrying out these reactions (24, 32). The reader is referred to Bonner (4) for a comprehensive review of pathways of carbohydrate metabolism in the fungi and the related actinomycetes.MATERIALS AN-D METHODS GROWTH AND PREPARATION OF THE FUNGUS: The organism used was the Burma 1 Da strain of Allomyces macrogynus (10). It was grown in liquid, agitated culture in 50 ml of medium in 125-ml Erlenmeyer flasks as described by AMachlis (27). The complete medium, as used in this study, is the same as the minimal medium of Machlis (27) except for the addition of 10i4 M L-glutamic acid. Low-sugar medium differs only in that the concentration of glucose is 0.05 % instead of 0.5 %. Each flask of medium was inoculated with 0.5 or 1.0 ml of mitospores and then incubated in the dark (except when room lights were turned on for observations) on a rotary shaker at 250 C for 48 to 96 hours. The mitospore inoculum was prepared as previously described (26).For the manometric determinations, the flasks of spherical plants were emptied over a wire screen or nylon cloth, the occasional large plaints removed, and the remaining retained plants rinsed with three or four washes of 0.04 M potassium phosphate buffer at pH 7.0. The rinsed plants from the requisite number of flasks were then composited, susl)ended in the solution to be used in the vessels and transferred to the Warburg vessels with a calibrated pipette having an opening approximately 5 mm in diameter.PREPARATION OF 'MITOCHONIDRIA: Initial trials to obtain mitochondria were unsuccessful using homogenizers of the Ten-Broeck and Potter-Elvehjem type, carborundum of several mesh sizes, and washed sand.At the suggestion of Dr. V. WV. Cochrane, ground Pyrex glass was tried and mesh sizes 20 through 50 were found to work well. The screened glass was washed with acid followed by distilled water until all acid was removed. It was then oven-dried. For a typical experiment 40 flasks of plants grown in complete medium for 80 to 96 hours were used. They were removed from the growth medium and washed on a nylon cloth over a Biuchner funnel with approximately 500 ml of cold 0.04 MI potassium phosphate buffer (pH 7.0 or 7.4). Excess buffer was pressed from the plants which were then ground in a cold room with 50 to 60 gm of ground glass and a final total of 150 to 180 ml of a solution containing 0.5 M sucrose, 0.1 M potassium phosphate, and 0.001 M potassium ethylenediamine tetraacetate at a pH o 7.0 or 7.4. Both pH 7.0 and 7.4 gave identical results.The...

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The path of carbon in photosynthesis. V. Paper chromatography and radioautography of the products

Cited by 98 publications

References 0 publications

Aminooxyacetate Stimulation of Glycolate Formation and Excretion by Chlamydomonas

Aminooxyacetate Stimulation of Glycolate Formation and Excretion by Chlamydomonas

Photoassimilation of organic compounds by autotrophic blue-green algae

Respiration of the Mycelia and Mitochondria of the Filamentous Watermold, Allomyces Macrogynus

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