The Passage of Oxygen through Isolated Sheets of Human Stratum Corneum

Penneys, Raymond; Felder, W.; Christophers, Enno

doi:10.3181/00379727-127-32860

Cited by 5 publications

(6 citation statements)

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“…Penney et al (1968) took sheets of isolated human stratum corneum and measured the diffusion of oxygen from a chamber of water equilibrated with air to a chamber that contained water deoxygenated by nitrogen [13]. It was shown that oxygen diffused through the stratum corneum, raising the oxygen partial pressure of the de-oxygenated water.…”

Section: Measurements Of Topical Oxygenationmentioning

confidence: 99%

Topical Dissolved Oxygen Penetrates Skin: Model and Method

Roe¹,

Gibbins²,

Ladizinsky³

2010

Journal of Surgical Research

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Section: Measurements Of Topical Oxygenationmentioning

confidence: 99%

Topical Dissolved Oxygen Penetrates Skin: Model and Method

Roe¹,

Gibbins²,

Ladizinsky³

2010

Journal of Surgical Research

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“…It is now thought, and supported by several studies, that skin can use oxygen from the atmosphere. [35][36][37] In vitro studies show that oxygen passes through the intact stratum corneum down to a depth of approximately 0.30 mm in the superficial dermis. 36,37 Similarly, studies have shown that the necessary transcutaneous oxygen level (TcpO 2 ) for normal skin is 30 mm Hg or greater with levels less than 30 mm Hg often leading to poor wound healing outcomes.…”

Section: Discussionmentioning

confidence: 99%

“…[35][36][37] In vitro studies show that oxygen passes through the intact stratum corneum down to a depth of approximately 0.30 mm in the superficial dermis. 36,37 Similarly, studies have shown that the necessary transcutaneous oxygen level (TcpO 2 ) for normal skin is 30 mm Hg or greater with levels less than 30 mm Hg often leading to poor wound healing outcomes. 38 Micro/nanobubbles are miniature gaseous bodies in fluid that can deliver oxygen to wounds.…”

Section: Discussionmentioning

confidence: 99%

A Quantitative Assessment of Wound Healing With Oxygenated Micro/Nanobubbles in a Preclinical Burn Model

et al. 2021

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Background Burns are devastating injuries, carry significant morbidity, and require long-term treatment or multiple reconstructive procedures. Wound healing and secondary insults caused by burn wound conversion are amendable to therapeutic intervention, where ischemia has been cited as one of the major factors (Dermatol Surg. 2008;34:1159–1169). Halting injury progression in the zone of stasis is crucial as conversion creates increased burn surface area and depth, leading to local and systemic consequences (J Burns Wounds. 2006;5:e2). Oxygen-carrying micro/nanobubbles, MNB(O2), offer a novel technology that can be used to effectively deliver oxygen to burn wounds and potentially counteract burn wound ischemia. Methods Topical irrigation with MNB(O2) of full-thickness burn wounds on a rodent model (n = 3) was compared against saline-treated controls (n = 3). Tissue structure (reduced scattering coefficient, μs'), oxyhemoglobin concentration (cHbO2), and tissue perfusion were quantified over the course of 28 days through spatial frequency domain imaging and laser speckle imaging. Histological samples taken at the end of the experiment were examined for evidence of wound healing. Results Findings in this preliminary study showed hastened healing with significant differences in spatial frequency domain imaging–measured μs' during wound healing (days 11–28) in MNB(O2) group. The healing “tipping point” seemed to occur at days 9 to 11 with increased collagen organization and increased cHbO2 occurring around that period confirming the gross healing improvements observed. In addition, histological evidence indicated that only the MNB(O2) burns had reached the remodeling phase by the end of 28-day study period. Conclusions These preliminary findings propose the potential of MNB(O2) as a topical method for improving burn wound healing.

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“…Penney et al in 1968 showed that sheets of isolated human stratum corneum allow diffusion of oxygen from a chamber of water equilibrated with air to a chamber that contained water deoxygenated by nitrogen. 24 It was shown that oxygen diffused through the stratum corneum, raising the oxygen partial pressure of the de-oxygenated water. The thickness of the stratum corneum was estimated to average 12 µm.…”

Section: Topical Oxygen and Skin Transmissionmentioning

confidence: 99%

<p>Assessment of Acceptability and Ease of Use of Haemoglobin Spray (Granulox<sup>®</sup>) in the Management of Chronic Wounds</p>

Kröger¹,

Gäbel²,

Juntermanns³

2020

CWCMR

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Wound healing is a complex and orchestrated process. Oxygen is one of the molecules that is required throughout the wound healing continuum for various underlying processes from innate immune response at the inflammatory phase to building of collagen type III at the remodelling phase. Therefore, it is not surprising that, due to primary diseases like venous insufficiency and diabetes mellitus, hypoxia caused by sustained lack of oxygen is common in chronic wounds. Adjunct to systemic therapies of the primary disease aimed at revascularizing the tissue, recently developed technologies are now available that provide topical oxygen-enhanced wound healing. Several devices are designed to provide pure oxygen directly to the wound either with or without pressure. Another approach to the provision of additional topical oxygen is to use haemoglobin to facilitate oxygen diffusion. This phenomenon was first shown more than 50 years ago, but it has only recently been discovered as a means of improving wound healing. In vitro and in vivo studies have shown that haemoglobin is capable of transporting oxygen outside of erythrocytes. Furthermore, several clinical studies have shown a beneficial effect on wound healing in different wound types. In this review, we summarise publicly available in vitro and in vivo clinical data and discuss the acceptability and ease of use of haemoglobin spray (Granulox ® ) in the management of chronic wounds. In conclusion, currently available data are promising, demonstrating that purified haemoglobin in an aqueous solution is able to serve as an oxygen shuttle by facilitating diffusion. No severe side effects have been reported in the literature, implying that it is a safe product. In clinical studies, it has been observed to improve the speed of healing of different types of chronic wounds. As a simple spray, it can be used as adjunct treatment in many standard-of-care settings with only a few limitations.

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The Passage of Oxygen through Isolated Sheets of Human Stratum Corneum

Cited by 5 publications

References 0 publications

Topical Dissolved Oxygen Penetrates Skin: Model and Method

Topical Dissolved Oxygen Penetrates Skin: Model and Method

A Quantitative Assessment of Wound Healing With Oxygenated Micro/Nanobubbles in a Preclinical Burn Model

<p>Assessment of Acceptability and Ease of Use of Haemoglobin Spray (Granulox<sup>®</sup>) in the Management of Chronic Wounds</p>

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