The partition of solutes between buffer solutions and solutions containing hyaluronic acid

Ogston, A. G.; Phelps, C. F.

doi:10.1042/bj0780827

Cited by 269 publications

(104 citation statements)

References 13 publications

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“…Most of the influx of protein in inflammatory fluids is probably due to an increase in capillary permeability. It is likely, however, that in normal and noninflammatory fluids at least some portion of serum protein is excluded from the articular cavity because of inability to penetrate the domain of the high molecular weight hyaluronic acid (30).…”

Section: Discussionmentioning

confidence: 99%

Immunoelectrophoretic assay for synovial fluid C3 with correction for synovial fluid globulin

Hasselbacher

1979

Arthritis & Rheumatism

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Synovial fluid C3 was measured by electroimmunoassay. When C3 was expressed as mg/ml, the amounts found in Reiter's disease, psoriatic arthritis, gout, and systemic lupus erythematosus were significantly different from degenerative arthritis. When C3 was corrected for total protein, the levels for rheumatoid arthritis, Reiter's disease, psoriatic arthritis, and systemic lupus were significantly different from degenerative arthritis. When C3 was corrected for synovial fluid globulin, only rheumatoid arthritis and systemic lupus were significantly different from degenerative arthritis. Correction of C3 for globulin increases the difference between rheumatoid arthritis and degenerative arthritis. A proportion of gouty fluids with a relative decrease in C3 is demonstrated. It is argued that correction of C3 for globulin is more meaningful than correction for total

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Section: Discussionmentioning

confidence: 99%

Immunoelectrophoretic assay for synovial fluid C3 with correction for synovial fluid globulin

Hasselbacher

1979

Arthritis & Rheumatism

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“…In the present experiments the mannitol space has been measured. Mannitol is likely to give a better estimate than inulin, which is probably hindered by steric barriers in the extracellular space (Ogston & Phelps, 1960;Goodford & Leach, 1966). Table 3 shows the mannitol space in muscles soaked for 1 hr in various media and expressed as ml./g.…”

Section: Sodium In Diaphragm 263mentioning

confidence: 99%

Sodium fluxes in diaphragm muscle and the effects of insulin and serum proteins

Creese¹,

Jenden²

1968

The Journal of Physiology

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SUMMARY1. Thin diaphragm muscles in dialysed serum maintained their total sodium at values similar to those found in vivo. The fibre sodium exchanged with a half-time of 5 min, and the flux was 1O p-mole.cm-2. sec-'. The internal sodium was less than 10 ,umoles/g fibre water and the ratio of exterr-al to internal sodium was at least 15. The calculated energy expenditure for sodium extrusion was less than 2 % of the resting metabolism.2. In physiological saline the half-time for exchange of fibre sodium was similar to that in serum, but in saline there was an increase in sodium permeability and a raised total and fibre sodium. 3. Muscles treated with insulin (0-02 u./ml.) showed a more rapid exchange of sodium compared with muscles in saline, with no change in the permeability to sodium. Insulin also affected sodium movements in the absence of external glucose.4. In saline the fraction of sodium which exchanged rapidly occupied 0-33 by volume of the muscle, after corrections for diffusion, and this value was similar to the mannitol space.5. Fibre diameters were measured in frozen sections. Muscles prepared by fixation and embedding showed marked shrinkage.

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“…The exclusion properties of mucopolysaccharide gels were investigated by Ogston & Phelps (1961) and Laurent (1964). In view of their individual molecular radii, it is not therefore surprising to find that inulin equilibrates within a smaller space than does raffinose which in turn equilibrates within a smaller space than does sucrose (I.S.…”

Section: Saccharide Spaces In the Gastrocnemius 553mentioning

confidence: 99%

The size of the sucrose, raffinose, and inulin spaces in the gastrocnemius muscle in the rat

Law¹,

Phelps²

1966

The Journal of Physiology

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SUMMARY1. The sucrose, raffinose and inulin spaces within the gastrocnemius of the female rat have been estimated at 12-0+ 2-2 %, 10-7 + 06 %, and 10-0 + 0-3 0/ respectively, expressed as a percentage of the total muscle volume.2. The mucopolysaccharide and hexosamine contents of the gastrocnemius have been found to be inversely dependent upon the weight of the muscle.3. The possibility that a relation exists between the saccharide spaces and the muscle musopolysaccharide content has been discussed in terms of saccharide molecular radii.

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The partition of solutes between buffer solutions and solutions containing hyaluronic acid

Cited by 269 publications

References 13 publications

Immunoelectrophoretic assay for synovial fluid C3 with correction for synovial fluid globulin

Immunoelectrophoretic assay for synovial fluid C3 with correction for synovial fluid globulin

Sodium fluxes in diaphragm muscle and the effects of insulin and serum proteins

The size of the sucrose, raffinose, and inulin spaces in the gastrocnemius muscle in the rat

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