The particle in the spider's web: transport through biological hydrogels

Witten, Jacob; Ribbeck, Katharina

doi:10.1039/c6nr09736g

Cited by 137 publications

(185 citation statements)

References 199 publications

Supporting

Mentioning

172

Contrasting

Order By: Relevance

“…Our model did not include goblet cells, mucus secretory cells, or submucosal glands to produce mucosal lining fluid (ie, mucus) . Mucin is the main structural component of mucus; these components interact with each other and with other components of mucus to form a meshlike structure . In addition to this meshlike structure, mucus captures foreign particles via an adhesion effect.…”

Section: Methodsmentioning

confidence: 99%

“…35 Mucin is the main structural component of mucus; these components interact with each other and with other components of mucus to form a meshlike structure. 12,36,37 In addition to this meshlike structure, mucus captures foreign particles via an adhesion effect. However, the density, viscosity, and elasticity of human mucus are not evident in the literature, not to mention the mucin-based meshlike structure within it.…”

Section: Mucosal Lining Fluidmentioning

confidence: 99%

See 1 more Smart Citation

Measuring the administered dose of particles on the facial mucosa of a realistic human model

Duan

Liu

et al. 2019

Indoor Air

View full text Add to dashboard Cite

Exposure to particulate contaminants can cause serious adverse health effects. Deposition on the facial mucosa is an important path of exposure, but it is difficult to conduct direct dose measurement on real human subjects. In this study, we propose an in vitro method to assess the administered doses of micron‐sized particles on the eyes and lips in which computed tomographic scanning and three‐dimensional printing were used to create a model that includes a face, oropharynx, trachea, the first five generations of bronchi, and lung volume. This realistic model of a face and airway was exposed to monodispersed fluorescent particles released from an incoming jet. The administered dose of particles deposited upon the eyes and lips, as quantified by fluorescence intensity, was determined via a standard wiping protocol. The results show that, in this scenario, the administered doses normalized by source were 2.15%, 1.02%, 0.88%, 2.13%, and 1.55% for 0.6‐, 1.0‐, 2.0‐, 3.0‐, and 5.0‐µm particles, respectively. The administered dose of large particles on the mucosa within a given exposure time has great significance. Moreover, the lips suffer a much greater risk of exposure than the eyes and account for more than 80% of total facial mucosa deposition. Our study provides a fast and economical method to assess the administered dose on the facial mucosa on an individual basis.

show abstract

Section: Methodsmentioning

confidence: 99%

Section: Mucosal Lining Fluidmentioning

confidence: 99%

Measuring the administered dose of particles on the facial mucosa of a realistic human model

Duan

Liu

et al. 2019

Indoor Air

View full text Add to dashboard Cite

show abstract

“…A similar model for transient immobilization events for a diffusive particle appears in Chen et al (2014), describing the behavior of viruses whose diffusivities in mucus are decreased by the presence of certain antibodies. (See also a review by (Witten and Ribbeck, 2017)). In both settings, the authors use a two-state model: in one state the particle of interest diffuses with the expected Stokes-Einstein drag; in the other state, the particle is immobilized.…”

Section: Skating Diffusion: Modeling and Inferencementioning

confidence: 99%

Assessing the Impact of Electrostatic Drag on Processive Molecular Motor Transport

Smith

McKinley

2018

Bull Math Biol

View full text Add to dashboard Cite

The bidirectional movement of intracellular cargo is usually described as a tug-of-war among opposite-directed families of molecular motors. While tug-of-war models have enjoyed some success, recent evidence suggests underlying motor interactions are more complex than previously understood. For example, these tug-of-war models fail to predict the counterintuitive phenomenon that inhibiting one family of motors can decrease the functionality of opposite-directed transport. In this paper, we use a stochastic differential equations modeling framework to explore one proposed physical mechanism, called microtubule tethering, that could play a role in this "co-dependence" among antagonistic motors. This hypothesis includes the possibility of a trade-off: weakly bound trailing molecular motors can serve as tethers for cargoes and processing motors, thereby enhancing motor-cargo run lengths along microtubules; however, this introduces a cost of processing at a lower mean velocity. By computing the small- and large-time mean-squared displacement of our theoretical model and comparing our results to experimental observations of dynein and its "helper protein" dynactin, we find some supporting evidence for microtubule tethering interactions. We extrapolate these findings to predict how dynein-dynactin might interact with the opposite-directed kinesin motors and introduce a criterion for when the trade-off is beneficial in simple systems.

show abstract

“…Mucins can physically and chemically (i.e. via intermolecular interactions) interact with each other and with other components of mucus to form a mesh-like structure (average pore size 10–500 nm)[3–5]. In addition to this mesh-like structure, mucus clearance and binding interactions can regulate microbe penetration, as well as drug and particle diffusion to the underlying epithelium[6, 7].…”

Section: Introductionmentioning

confidence: 99%

Mucus models to evaluate the diffusion of drugs and particles

Lock

Carlson

2018

Advanced Drug Delivery Reviews

163

119

View full text Add to dashboard Cite

Mucus is a complex hydrogel that acts as a natural barrier to drug delivery at different mucosal surfaces including the respiratory, gastrointestinal, and vaginal tracts. To elucidate the role mucus plays in drug delivery, different in vitro, in vivo, and ex vivo mucus models and techniques have been utilized. Drug and drug carrier diffusion can be studied using various techniques in either isolated mucus gels or mucus present on cell cultures and tissues. The species, age, and potential disease state of the animal from which mucus is derived can all impact mucus composition and structure, and therefore impact drug and drug carrier diffusion. This review provides an overview of the techniques used to characterize drug and drug carrier diffusion, and discusses the advantages and disadvantages of the different models available to highlight the information they can afford.

show abstract

The particle in the spider's web: transport through biological hydrogels

Cited by 137 publications

References 199 publications

Measuring the administered dose of particles on the facial mucosa of a realistic human model

Measuring the administered dose of particles on the facial mucosa of a realistic human model

Assessing the Impact of Electrostatic Drag on Processive Molecular Motor Transport

Mucus models to evaluate the diffusion of drugs and particles

Contact Info

Product

Resources

About