The Partial Purification of Staphylocoagulase and the Effect of Certain Presumptive Inhibitors upon Its Plasma-coagulating Action

Purification of Staphylococcal Coagulase

“…A decrease in activity was also noticed upon storage of the lyophilized electrophoretically purified enzyme for 3 months in a desiccator at 4 C. It is conceivable that impurities formed a protective coat around the enzyme molecules. Walker et al (1948) reported that a preparation of crude coagulase resisted autoclaving at 120 C. Tager (1948) found that the thermosensitivity of the clotting enzyme increased considerably with its degree of purity.…”

Staphylococcal Hyaluronidase

“…The double maximum may be real and may offer confirmation of Rogers' (1948) concept of more than one hyaluronidase. An alternative explanation is that it is only apparent, and caused by the coprecipitation of other materials, particularly procoagulase (Walker et al, 1948), in the pH range 4.5 to 3.5.…”

“…Tryptic digest (pH 7.6) was selected as the culture medium for the following reasons: (1) It has been established (Rogers, 1945) that optimal formation of hyaluronidase occurs only in well-buffered media. (2) Previous studies of staphylococcal coagulase production, made in this laboratory (Walker et al, 1947(Walker et al, , 1948, have shown that this medium is highly satisfactory for the growth of the staphylococcus.…”