The partial mitochondrial sequence of the Old World stingless bee, Tetragonula pagdeni

Thummajitsakul, Sirikul; Silprasit, Kun; Klinbunga, Sirawut; Sittipraneed, Siriporn

doi:10.1007/s12041-013-0243-1

Cited by 7 publications

(16 citation statements)

References 18 publications

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“…As the typical mitogenome, it included 37 genes and one D-loop region, with these units distributed on both strands of the mitogenome (in detail see Table 1). It is worth mentioning that the order of these genes in our mitogenome is identical to the order in the partial mitogenome reported by Thummajitsakul et al (2013). As seen in other insects (Liu et al 2021), the mitochondrial genome of T. pagdeni has a substantially greater AT richness (76.77 %) than the GC value.…”

Section: General Mitogenome Featuressupporting

confidence: 82%

“…pagdeni mitogenome reported by Thummajitsakul et al . (2013) tthe lengths of them were 762 bp and 1,351 bp, respectively. The length difference of ribosomal RNA genes between the two T .…”

Section: Resultsmentioning

confidence: 99%

“…It is worth mentioning that the order of these genes in our mitogenome is identical to the order in the partial mitogenome reported by Thummajitsakul et al . (2013). As seen in other insects (Liu et al .…”

Section: Resultsmentioning

confidence: 99%

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Unusual mitochondrial tRNA rearrangements in stingless bee Tetragonula pagdeni and phylogenetic analysis

Wang

Yang

Zhao

et al. 2022

Entomological Science

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Tetragonula pagdeni (Hymenoptera: Meliponini) is a widely distributed and economically important stingless bee. The taxonomic status and evolution of this species have not been fully understood. We sequenced the mitogenome of T. pagdeni and analyzed its characteristics including gene rearrangements in present work, which will provide help for better protection and utilization of this germplasm resource. The mitochondrial genome sequence was obtained by establishing library and high-throughput sequencing. Using all protein coding sequences joined, the phylogenetic analysis demonstrated that T. pagdeni has the closest kinship with Lepidotrigona species, and then has a second closest kinship with the Melipona stingless bees. All the stingless bee species (Meliponini) analyzed formed a single clade, and among them the T. pagdeni mitogenome exhibited the highest gene rearrangement score, suggesting this species has an overactive evolutionary history. Especially, an unusual tRNA-Gly translocation was observed in T. pagdeni mitogenome, which broke the super-conserved atp8-atp6-cox3-G block in Hymenoptera mitogenomes. The translocated tRNA-Gly moved to a position next to the D-loop control region, and whether this translocation has a functional effect to the widely distributed T. pagdeni need further exploration. The variations of gene positions in mitogenome could also provide useful information to clarify the phylogenetic relations in Meliponini.

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Section: General Mitogenome Featuressupporting

confidence: 82%

“…pagdeni mitogenome reported by Thummajitsakul et al . (2013) tthe lengths of them were 762 bp and 1,351 bp, respectively. The length difference of ribosomal RNA genes between the two T .…”

Section: Resultsmentioning

confidence: 99%

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Unusual mitochondrial tRNA rearrangements in stingless bee Tetragonula pagdeni and phylogenetic analysis

Wang

Yang

Zhao

et al. 2022

Entomological Science

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“…The materials used in this study were a specimens of stingless bees species (five individuals/location), ethanol 96%, chloroform, isopropanol, TE buffer, CTAB 3%, DNA loading dye. The 16S rRNA DNA mitochondrial primer pairs employed in the study is LR13107-F as a forward primer: 5'-TGG CTG CAG TAT AAC TGA CTG TAC AAA GG-3' and LR12647-R as reverse primer: 5'-GAA ACC AAT CTG ACT TAC GTC GAT TTG A-3' (Thummajitsakul et al, 2013), PCR Kit (Go Taq Green©), Nuclease free water, DNA Ladder 1 kb and 100 bp, Promega Wizard sv gel© and PCR clean/up system kit©, 1% electrophoretic gel, TAE buffer, EtBr.…”

Section: Tools and Materialsmentioning

confidence: 99%

“…The amplification of Polymerase Chain Reaction (PCR) and DNA Sequencing was conducted using the mitochondrial 16S rRNA gene primers as described previously (Thummajitsakul et al, 2013). The PCR were completed in 35 cycles of 30 µl reaction volume, based on Na-Nokorn et al (2006) and Mahendran et al (2006).…”

Section: Dna Amplification and Sequencingmentioning

confidence: 99%

Molecular Phylogeny of Stingless Bees in the Special Region of Yogyakarta Revealed Using Partial 16S rRNA Mitochondrial Gene

Trianto

Purwanto

2020

BuletinPeternak

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Systematics of bees has developed rapidly. Several studies have attempted to infer the kinship between each group of bee. One way is the use of phylogenetic analysis using molecular data. This study explains the phylogenetic relationship of stingless bees in the Special Region of Yogyakarta, Indonesia based on the 16S rRNA gene. The research has been carried out in five districts and cities in the region from June to September 2019. In the study, the stages implemented include; place determination of stingless bees sampling, sampling, and molecular identification (DNA extraction, DNA amplification, and sequencing), followed by the data analysis using NCBI database and MEGA X software. The result of this study indicated that among seven morphospecies from Yogyakarta, there are six species of stingless bees that have a closest genetic relationship with the same species data from Genbank, namely Tetragonula laeviceps, T. iridipennis, T. sapiens, T. sarawakensis, Lepidotrigona terminata, and Heterotrigona itama. Since the genetic distances of T. laeviceps, T. iridipennis, T. sapiens, are wider than 3,5%, the data indicate that there is a possibility that the three morphospecies are actually belong to a different species with a similar morphology. Meanwhile, for the morphospecies T. biroi, the closest hit is on T. pagdeni 16S rRNA DNA because the T. biroi 16S rRNA DNA data is not available on the database. This study is expected to contribute to the preservation and utilization of one of Indonesia's important biodiversity resources.

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Molecular detection of the pathogen of Apis mellifera (Hymenoptera: Apidae) in honey in Indonesia

Aditya

Purwanto

2023

Biodiversitas

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Abstract. Aditya IGMRA, Purwanto H. 2023. Molecular detection of the pathogen of Apis mellifera (Hymenoptera: Apidae) in honey in Indonesia. Biodiversitas 24: 2612-2622. Apis honey bees play a major role in global crop pollination. The honey bee population faces various threats, one being entomopathogens, responsible for 13% of honey bees' declining population cases. Some major pathogens are Nosema sp., Melissococcus plutonius, and Paenibacillus larvae. Data on the prevalence of these pathogens is not available in Indonesia. Therefore, this study aimed to detect entomopathogen in Java, Bali, and Sumatra honey. We extracted 30 honey samples from Java, Bali, and Sumatra in this study. DNA from honey amplified by conventional Polymerase Chain Reaction (PCR) to detect Nosema apis, Nosema ceranae, M. plutonius, and P. larvae. Amplicons were sequenced to confirm the species of pathogens detected. Results showed that N. ceranae has a 20% prevalence, found in Java and Bali; M. plutonius was, for the first time, found in Indonesia and only in Java, with a 3.33% prevalence. In comparison, this study didn't find N. apis and P. larvae. Environmental factors were studied for their correlation with the incidence of pathogens. From several factors, only precipitation significantly correlates with the occurrence of N. ceranae and M. plutonius, while other factors do not. This research concludes that honey can be used as source of DNA for pathogens detection and monitoring in a certain area globally.

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The partial mitochondrial sequence of the Old World stingless bee, Tetragonula pagdeni

Cited by 7 publications

References 18 publications

Unusual mitochondrial tRNA rearrangements in stingless bee Tetragonula pagdeni and phylogenetic analysis

Unusual mitochondrial tRNA rearrangements in stingless bee Tetragonula pagdeni and phylogenetic analysis

Molecular Phylogeny of Stingless Bees in the Special Region of Yogyakarta Revealed Using Partial 16S rRNA Mitochondrial Gene

Molecular detection of the pathogen of Apis mellifera (Hymenoptera: Apidae) in honey in Indonesia

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