The ParD^‐ mutant of Escherichia coli also carries A_am mutation. The complete sequence of gyrA

Abstract

The phenotype of a recently-described mutant (OV6), conditionally defective in chromosome partitioning and septal positioning, was originally thought to be due to a new gene (parD) mapping at 88.4 min. We have now shown that, in addition to the parD mutation, OV6 carries a gyrAam mutation and that this mutation is probably responsible for the gross phenotype of the mutant. We have cloned the gyrA gene, identified the GyrA protein, sequenced the gyrA gene and flanking genes, cloned and sequenced the gyrAam mutation, and identified its truncated product. In addition, we have identified the transcriptional start point of the gyrA gene. The E. coli GyrA protein has extensive homologies with Gyrase proteins of other organisms and weak sequence homologies with some eukaryotic cytoskeletal proteins.

Isolation and characterization of Escherichia coli mutants affected in aerobic respiration: the cloning and nucleotide sequence of ubiG: Identification of an S-adenosylmethionine-binding motif in protein, RNA, and small-molecule methyltransferases

“…Our sequence (Fig. 3) agrees with that of Hussain et al (1987). Several potential long ORFs, shown in Fig.…”

“…Some of the discrepancies among these reported sequences are discussed in the text. Note that after nucleotide number 1679 in the present sequence, it is no longer similar with that reported by Hussain et al (1987). The clones used for complementation experiments are also shown.…”

“…The ClaI site is not part of the reported gyrA sequence and may be a cloning artefact. The sequences reported by Hussain et al (1987) and Swanberg & Wang (1987) also disagree with each other at a number of sites; for example, at nucleotide number 1029 and 1045, both Gs are reported as A by Swanberg & Wang (1987), and the A at nucleotide 1087 is missing in their sequence. Our sequence (Fig.…”

“…2(b) shows the extent to which the complete nucleotide sequence of the ClaI-EcoRV fragment (Fig. 3) includes regions studied by Hussain et al (1987), Swanberg & Wang (1987 and Yoshida et al (1988). These workers did not identify the ORF that we assign to ubiG (see below).…”

Deletion analysis of cspA of Escherichia coli: requirement of the AT‐rich UP element for cspA transcription and the downstream box in the coding region for its cold shock induction

“…The AUG codons in cspA, cspB and cspG are at the fifth position in the coding sequence. The number of potential basepairs between DB and ADB is given for each mRNA: cspA (Goldstein et al, 1990), cspB (Lee et al, 1994), cspG (Nakashima et al, 1996), csdA (¼deaD, Toone et al, 1991), rbfA (Dammel and Noller, 1995), hns (Pon et al, 1988), pnp (Regnier et al, 1987), nusA (Ishii et al, 1984), infB (Sacerdot et al, 1984), gyrA (Hussain et al, 1987), cspC (Lee et al, 1994;Yamanaka et al, 1994), cspD (Gottesman et al, 1990) and cspE (Yamanaka et al, 1994). a.…”